Screening and preliminary analysis of an endosperm-specific expression gene in rice(<i>Oryza sativa</i> L.)

ZHANG Hui; HU Chang-quan; LIU Hua-qing; LI Su-yi; LIU Ci-tao; PAN Da-ren; WANG Feng

Volume 24 Issue 1

Nov. 2011

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Article Navigation > Fujian Journal of Agricultural Sciences > 2009 > 24(1): 6-10

ZHANG Hui, HU Chang-quan, LIU Hua-qing, LI Su-yi, LIU Ci-tao, PAN Da-ren, WANG Feng. Screening and preliminary analysis of an endosperm-specific expression gene in rice(Oryza sativa L.)[J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 6-10.

Citation:

ZHANG Hui, HU Chang-quan, LIU Hua-qing, LI Su-yi, LIU Ci-tao, PAN Da-ren, WANG Feng. Screening and preliminary analysis of an endosperm-specific expression gene in rice(Oryza sativa L.)[J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 6-10.

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Screening and preliminary analysis of an endosperm-specific expression gene in rice(Oryza sativa L.)

1.
College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350007, China;
2.
Fujian Key Laboratory of Genetic Engineering for Agriculture, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
3.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350013, China

Received Date: 2008-12-03
Rev Recd Date: 2009-01-05
Publish Date: 2009-02-15

Abstract

Abstract

A promoter trapping line(accession no.w9101),in which the β-glucuronidase(GUS)reporter gene was endosperm-specific expression in both T0 and T1 generations,was identified from a promoter trapping population in rice(Oryza sativa L.ssp.indica).Genetic analysis and the Southern blot hybridization of the marker gene indicated that w9101 was a trapping line with a copy of T-DNA insertion.The flanking sequence of T-DNA insertion site was isolated by TAIL-PCR in w9101.The BLAST result of the sequence showed that the T-DNA insertion site was located in the promoter sequence of a putative peptide transporter gene in chromosome 6 of rice.It was tentatively named as PTR9101.RT-PCR analysis of the PTR9101 gene revealed that it was specifically expressed in rice endosperm.Further analysis by bioinformatics method indicated that the PTR9101 protein was hydrophobic with 12 α-helix membrane-spanning domain and similar to that of CHL1(The Nitrate Transporter AtNRT1.1)in Arabidopsis.It was thus concluded that PTR9101 was a hydrophobic peptide transporter gene.
- Rice(Oryza sativa L.),
- endosperm,
- promoter trapping line,
- PTR9101

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References(6)

References

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