Identification of the pathogens causing root rot on <i>Polygonatum cyrtonema</i> Hua in Fujian

GAO Wenli; SU Hailan; LIN Fengfang; NIU Yuqing; ZHU Yujing; LIU Bo; CHENG Xi; XIAO Rongfeng

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GAO W L, SU H L, LIN F F, et al. Identification of the pathogens causing root rot on Polygonatum cyrtonema Hua in Fujian [J]. Fujian Journal of Agricultural Sciences，2024，39（5）：1−9

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GAO W L, SU H L, LIN F F, et al. Identification of the pathogens causing root rot on Polygonatum cyrtonema Hua in Fujian [J]. Fujian Journal of Agricultural Sciences，2024，39（5）：1−9

GAO W L, SU H L, LIN F F, et al. Identification of the pathogens causing root rot on Polygonatum cyrtonema Hua in Fujian [J]. Fujian Journal of Agricultural Sciences，2024，39（5）：1−9

Citation:

GAO W L, SU H L, LIN F F, et al. Identification of the pathogens causing root rot on Polygonatum cyrtonema Hua in Fujian [J]. Fujian Journal of Agricultural Sciences，2024，39（5）：1−9

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Identification of the pathogens causing root rot on Polygonatum cyrtonema Hua in Fujian

GAO Wenli^{1, 2
,},
SU Hailan³,
LIN Fengfang³,
NIU Yuqing³,
ZHU Yujing³,
LIU Bo²,
CHENG Xi^{1
,
,},
XIAO Rongfeng^2
,

1.
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University,　350002, Fuzhou, Fujian　350002, China
2.
Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
3.
Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China

Received Date: 2023-11-14
Rev Recd Date: 2024-01-05

Available Online: 2024-06-26

Abstract

Abstract

: Objective The purpose of this paper is to clarify the root rot incidence rate of Polygonatum cyrtonema Hua in Fujian area and identify the causing pathogens, which will provide a theoretical basis for the control of root rot disease. Methods The incidence of root rot were investigate in the three planting areas of P. cyrtonema in Fujian province. The roots and rhizomes with typical root rot symptoms were collected from the fields. The pathogens were isolated and identified with morphology, molecular biology and pathogenicity test. Results The average incidence of root rot was about 10.50% in the seedlings phase and 17.65% in the rhizomes during harvesting phase. A total of 98 isolates were obtained from the diseased samples. The pathogens were finally identified as Fusarium oxysporum, F. solani and F. fujikuroi based on morphological characteristics, FOF1/FOR1 and F8/R8 PCR amplification of specific primer, and sequence analysis of translation elongation factor-1α (tef-1α) gene. The separation frequencies of the tree pathogens were 75.51%, 20.41% and 4.08%, respectively. Inoculating the pathogens on the healthy rhizomes reproduced the same symptoms previously observed in the field. Pathogenicities of the tree pathogens were confirmed by fulfilling the Koch’s postulates. Conclusion It was confirmed that F. oxysporum, F. solani and F. fujikuroi were the pathogens of root rot of P. cyrtonema in Fujian province. This is the first report of F. fujikuroi causing root rot of P. cyrtonema in China.
- Polygonatum cyrtonema Hua,
- Root rot,
- Fusarium oxysporum,
- Fusarium solani,
- Fusarium fujikuroi

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References(25)

References

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