Multiplex-PCR analyses and seasonal changes of virulence genes of E.coli isolated from Min river watersheds in Fuzhou

Toxic genes of the potential pathogenic E.coli collected in different seasons from Min River watersheds in Fuzhou were analyzed using Multiplex-PCR.The results indicated that 840 out of 2015 E.coli isolates were potential pathogens classified as EHEC,EAEC,EPEC and ETEC groups.Ten virulence genes were detected with dominant genes,aidA-1,astA and sepA.The numbers of bacteria,E.coli and E.coli carrying virulence genes,increased in the spring and summer,peaked in the fall,and decreased in the winter.