Mitochondrial Genome Characterization and Phylogenomic analyses of Yellowfin Puffer（<i>Takifugu xanthopterus</i>）

ZHU Chunyue; HU Zongyun; ZHANG Jian; ZHANG Boxu; LIU Zhonghang; YANG Peimin

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ZHU C Y, HU Z Y, ZHANG J, et al. Mitochondrial Genome Characterization and Phylogenomic analyses of Yellowfin Puffer（Takifugu xanthopterus） [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−9

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ZHU C Y, HU Z Y, ZHANG J, et al. Mitochondrial Genome Characterization and Phylogenomic analyses of Yellowfin Puffer（Takifugu xanthopterus） [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−9

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Mitochondrial Genome Characterization and Phylogenomic analyses of Yellowfin Puffer（Takifugu xanthopterus）

Liaoning Institute of Freshwater Fisheries /Liaoning Key Laboratory of Aquatic Animal Diseases Control, Liaoyang, Liaoning　111000

Received Date: 2023-10-30
Rev Recd Date: 2024-02-01

Available Online: 2024-06-26

Abstract

Abstract

Objective Molecular genetics of Yellowfin Puffer （Takifugu xanthopterus） in Dayang River of China was studied, and provide basic data for genetic diversity and phylogenetic analysis of germplasm resources of T. xanthopterus. Method The complete mitochondrial genome sequence of T. xanthopterus was obtained by high-throughput sequencing technology, and the structure of mitochondrial genome was analyzed. Result The length of the complete mitochondrial genome was determined to be 16444bp （GenBank accession no. MT560586） consisting of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, 1 control region and O_L. Thirteen protein-coding genes started with ATG as a start codon except for the gene cox1 started with a special GTG codon, whereas Cox2 ended with codon T, the preference codon （RSCU＞2） including the codon Arg （CGA） and Ser （UCC）. All 22 tRNA genes present the typical clover leaf secondary structure except trnS1. Taking Monotreta leiurus as outgroup, the complete mitochondrial genome sequences of 16 species of Fugu were analyzed for evolutionary relationship. The phylogenetic tree constructed by Bayes software showed that T.xanthopterus had closely relationship with Takifugu chrysops and Takifugu pardalis. Conclusion The mitochondrial genome structure and gene order of T.xanthopterus conform to the typical structure and arrangement characteristics of fish genome.
- Takifugu xanthopterus,
- Mitochondrial genome,
- phylogenetic analysis

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References(41)

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