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Volume 27 Issue 9
Nov.  2012
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GE Jun-qing, YANG Jin-xian, LI You-juan, CHEN Qiang, LIN Tian-long. Polymerase Chain Reaction for the Detection of Herpesvirus Anguillae in Eel Viral Disease Samples[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 961-964.
Citation: GE Jun-qing, YANG Jin-xian, LI You-juan, CHEN Qiang, LIN Tian-long. Polymerase Chain Reaction for the Detection of Herpesvirus Anguillae in Eel Viral Disease Samples[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 961-964.
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Polymerase Chain Reaction for the Detection of Herpesvirus Anguillae in Eel Viral Disease Samples

  • Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

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  • Received Date: July 12, 2012
  • Revised Date: August 05, 2012
    Graphical Abstract
    • Abstract
  • Diseased eels collected between 2006-2012 were classified by pidemiological, clinical symptoms and pathological characteristics, and 36 samples suspected to be infected by virus were identified. Total DNA was extraced from liver, spleen or kidney, and used for herpesvirus anguillae (HVA) detection. The results showed that herpesvirus anguillae were detected from 6 samples, and the disease of "Skin Mucous Debonding", "Septicemia", "Red Head", "Gill Rotten", which causing huge losses to the eel aquaculture industry, might caused by HVA; the amplified DNA sequence were exactely the same with the sequence from the starin of Taiwan, Japan and Europe, which means they might be the identical strain.
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    • References (5)
  • VAN G V, BALLIEUX B, WILLEMZE R, et al. Hematology patterns of migrating European eels and the role of EVEX virus[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2005, 140(1): 97-102.
    JAKOB E, NEUHAUS H, STEINHAGEN D, et al. Monitoring of Herpesvirus anguillae (HVA) infections in European eel,Anguilla anguilla (L.), in northern Germany[J]. J Fish Dis, 2009, 32(6): 557-561.
    HAENEN O L M, IJKSTRA S G D, VAN TULDEN P W , et al.Herpesvirus anguillae (HVA) isolations from disease outbreaks in cultured European eel, Anguilla anguilla in The Netherlands since 1996[J]. Bull Eur Ass Fish Pathol, 2002, 22(4): 247-257.
    UENO Y, KITAO T, CHEN S N, et al. Characterization of a herpes-like virus isolated from cultured Japanease eels in Taiwan[J]. Fish Pathology, 1992, 27: 7-17.
    王存国, 黎向阳. 鳗鱼病毒性烂鳃病的防治[J]. 中国水产, 2002, (11): 82.
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