Detection of Sugarcane yellow leaf virus with RT-PCR in Chewingcane

According to the SCYLV coat protein (CP) gene sequences from GenBank, the specific primers were designed to amplify SCYLV CP gene from Fujian chewingcane (Badila) by RT-PCR..The PCR product contained 608 nucleotides. The results of nucleotide and amino acid sequence analysis showed that the PCR product contained the full length of SCYLV coat protein, which coded 196 amino acids. The CP gene sequences in this test have more than 96% homologous with CP genes of other SCYLV isolates from China and other countries. Phylogenetic tree suggested that the eighteen isolates were classified into three types according to the nucleotides sequence of SCYLV CP. The SCYLV-FJBadila belong to the BRA-PER genotypes.We sampled and amplified the CP genes from the chewingcane fields in LongWen, ChangTai, TongAn, etc of Fujian province, the result showed that the chewingcanes in Fujian province were universal infected with SCYLV.