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Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Diseases Control Techology Development Center, Fuzhou, Fujian 350013, China
Hps antibody of 378 serum sample from 23 pig farm in 8 city of Fujian Province was detected by the indirect ELISA reagent kit; And Hps was separated and identified by use of TSA plate with removal of fine horse blood from the collected disease materials of swine suspected infecting Hps; And the serotype of separated strains was classified by the Agar diffusion test; And a pair of specific primers was synthesized by referring to ompP5 gene of HS0165 strain signed in GenBank, and the ompP5 gene was amplified using Hps DNA extracted as a template by PCR method, cloned, sequenced and blasted. The results showed that the positive rate in 23 field from Fujian Province was to 100%, and the positive rate of the herd was to 36.78%; 52 isolates from the swine of suspected infecting Hps had been separated and identified, and were regarded as serotype 2, 4, 5, 13 and non-serotype respectively, in addition, the serotype 13 was the most; Compared the GenBank, the homology of ompP5 of 3 isolates are respectively up to 93%.
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