Cloning and Expression Analysis for Color Related Genes in Flower of Oncidium Sharry Baby.

Three flower color related genes(CHS、ANS、DFR)were cloned from the red flower sepals of Oncidium Sharry Baby with homology sequence cloning. The length of their conservative sequences were 611 bp、288 bp and 554 bp. Sequence analysis showed that these genes revealed high homology with the color related genes from other plants, ranging from 77%~89%、72%~99% and 57%~96% for CHS、ANS and DFR, respectively. Results of RT-PCR analysis showed that the transcripts of three genes were detected from red sepals but not in yellow labellum and leaves. The expression level of three genes was high in the bud and early flowering stages, and then decreased in blooming.