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Volume 27 Issue 3
Apr.  2012
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LIN Feng-qiang, ZHU Xiao-li, CHEN Shao-ying, WANG Shao, CHEN Shi-long, CHENG Xiao-xia, JIANG Bin, LI Zhao-long. Molecular Epidemiology of Muscovy Duck Reovirus[J]. Fujian Journal of Agricultural Sciences, 2012, 27(3): 217-221.
Citation: LIN Feng-qiang, ZHU Xiao-li, CHEN Shao-ying, WANG Shao, CHEN Shi-long, CHENG Xiao-xia, JIANG Bin, LI Zhao-long. Molecular Epidemiology of Muscovy Duck Reovirus[J]. Fujian Journal of Agricultural Sciences, 2012, 27(3): 217-221.
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Molecular Epidemiology of Muscovy Duck Reovirus

  • 1.

    Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian 350013, China;

  • 2.

    Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

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  • Received Date: January 30, 2012
  • Revised Date: February 14, 2012
    Graphical Abstract
    • Abstract
  • The primers were designed to amplify the S1 and S4 gene according to the muscovy duck reovirus(MDRV) sequencein Genbank. S1 and S4 gene of MDRV strains isolated in China were cloned and then sequenced and analyzed. The results of showed S4 and S1 gene was 992 bp and 819 bp in length, respectively; the nucleotide sequence of S1 segment shared 92.2%-96.7% identity in China strains, but 92.2%-96.7% identity with France strain, 89026; and 76%-78% identity with ARV strain, S1133. The nucleotide suquence of S4 segment shared 95.2%-99.3% idnentity in China stains, but 92.3%-94.5% identity with France strain, 89026, and 21.2%-21.5% identity with ARV strain,S1133. Phylogenetic analysis demonstrated that MDRV was quite different from ARV. The Ten MDRV strains isolated in China has no distinct difference, but they were different from 89026 (isolated in France). The study provide the basic research on the prevention with muscovy duck reovirusis.
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    • References (3)
  • 陈少莺,胡奇林,程晓霞,等.番鸭肝白点病病理学研究[J].福建农业学报,2002, 17(4): 220-222.
    JOSE M C, CLUAUDIA G L, VIKRAM N, et al. Possible involvement of the double-stranded RNA-binding core protein σA in the resistance of avian reovirus to interferon[J]. Am Soci Micr, 2000,67:1-9.
    郭东春,耿宏伟,刘明,等. 番鸭呼肠孤病毒σC编码基因在sf-9昆虫细胞中的表达[J]. 中国兽医科学,2007,37(5):374-377.
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