Collection, Identification and Phylogenetic Diversity of <em>Bacillus</em> species in Soil Samples from Xinjiang

WANG Zi-xuan; LIU Bo; LIN Ying-zhi; LIU Guo-hong

Volume 27 Issue 2

Mar. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2012 > 27(2): 187-195

WANG Zi-xuan, LIU Bo, LIN Ying-zhi, LIU Guo-hong. Collection, Identification and Phylogenetic Diversity of Bacillus species in Soil Samples from Xinjiang[J]. Fujian Journal of Agricultural Sciences, 2012, 27(2): 187-195.

Citation:

WANG Zi-xuan, LIU Bo, LIN Ying-zhi, LIU Guo-hong. Collection, Identification and Phylogenetic Diversity of Bacillus species in Soil Samples from Xinjiang[J]. Fujian Journal of Agricultural Sciences, 2012, 27(2): 187-195.

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Collection, Identification and Phylogenetic Diversity of Bacillus species in Soil Samples from Xinjiang

1.
Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2.
Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

Received Date: 2011-11-24
Rev Recd Date: 2011-12-10
Publish Date: 2012-02-29

Abstract

Abstract

The present study dealt with the collection, identification and phylogenetic diversity of Bacillus species in soil samples from Xinjiang. Fourteen soil samples were used for the microbial identification based on their fatty acid compositions and 16S rDNA sequences. As a result, 53 strains were isolated and identified as 13 species including B. megaterium,B. cereus,B. subtilis,B. alcalophilus,B. viscosus,B. licheniformis,B. atrophaeus, etc. The phylogenetic tree analysis clustered their 16S rDNA sequences for genetic relationship among them. Based on the diversity indices, the Bacillus counts in soils from different locations were found to vary significantly. For instance, the number in Alaer was greater than those in Xinjiang Kashen, Akesu and Takelamagan, in that order. Even the same species in the soil from a same locality could differ significantly. For example, Bacillus cereus was 5.230105 cfug-1 in Akesu, but only 0.7103 cfug-1 in Takelamagan. Bacillus existed abundantly in Xinjiang, and the varieties and quantities in the soils also varied greatly.
- Bacillus,
- Xinjiang,
- phylogenetic diversity,
- 16S rRNA gene

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References

[1]	SALAMANCAEF,RAUBUCHM,JOERGENSENRG.Micmbia reaction of secondary tropical forest soils to the addition of leaf litter[J]. Appl Soil Ecol, 2006, 31: 53-61.
[2]	TORRESAPA, ABRILAAB, BUCHERBEH. Microbial succe-ssion in litter decomposition in the semi. Arid Chaco woodland [J]. Soil Biol Bioehem, 2005, 37: 49-54.
[3]
[4]	张英, 郭良栋, 刘润进. 都江堰地点丛枝菌根真菌多样性与生态研究[J]. 植物生态学报, 2003, 27(4): 537-544.