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Volume 27 Issue 2
Mar.  2012
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ZHOU Zhi-lin, TANG Jun, CAO Qing-he, ZHAO Dong-lan, SHI Xin-min, YANG Feng. Tissue Culture and Micro-tuber Induction of Dioscorea opposita, Jiu Jin Huang[J]. Fujian Journal of Agricultural Sciences, 2012, 27(2): 144-148.
Citation: ZHOU Zhi-lin, TANG Jun, CAO Qing-he, ZHAO Dong-lan, SHI Xin-min, YANG Feng. Tissue Culture and Micro-tuber Induction of Dioscorea opposita, Jiu Jin Huang[J]. Fujian Journal of Agricultural Sciences, 2012, 27(2): 144-148.
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Tissue Culture and Micro-tuber Induction of Dioscorea opposita, Jiu Jin Huang

  • Xuzhou Institute of Agricultural and Sciences of the Xuhuai District, Xuzhou, Jiangsu 221121, China

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  • Received Date: August 02, 2011
  • Revised Date: September 14, 2011
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    • Abstract
  • Using yam bulbils for tuber propagation not only benefits the rejuvenation of Dioscorea opposita but also reduces the planting cost. Thus, conditions of the micro-tuber induction for the yams were studied. The shoot tips were cultured in MS medium with additions of varying concentrations of NAA/6-BA. Different treatments using NAA/KT, active carbon and sucrose were applied for the experimentation. The results indicated the optimal medium composition for the shoot tip induction to be: MS + 0.1 mgL-1 NAA+ 2.0 mgL-1 6-BA+ 0.2 gL-1 AC+ 30.0 gL-1 sucrose+ 8.0 gL-1 agar.The resultant plant regeneration rate was 91.7%. The optimal medium formula for the micro-tuber induction was: MS +0.1 mgL-1 NAA+ 1.0 mgL-1 KT+ 2.0 gL-1 AC+ 50.0 gL-1 sucrose+ 8.0 gL-1agar.The regenerated plants from the micro-tubers not only maintained the characteristics of the parent species but also improved its commercial appealing.
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    蔡建荣,曾军,张志勇,等.怀山药茎段组织培养及增殖的研究[J].福建农业科技,2002,(2):14-15.
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