Effect of Culture Medium with Dietary Fiber in Selenium Bioconversion by Pleurotus geesteranus

To study the bioconversion efficiency of Se by submerged culture of Pleurotus geesteranus,liquid medium with wheat bran and kudzu(Radix Puerariae)fiber were compared respectively with their filtration liquid in medium.The observation showed that the bioavailability of insoluble fibrous particles in medium was important to the primary colonization,elongation and development of mycelium pellets.With 3% wheat bran and 5% kudzu fiber in medium(W/V),the maximum mycelium biomass obtained was 22.4 mgL-1 and 27.5 mgL-1 and the bioconversion efficiency of Se was 71.05% and 96.16%,respectively.Comparing with wheat bran filtration in medium as control,mycelium biomass in fibrous medium was increased by 1-2 times and se conversion rate increased 37%-45%.