Rapid Detection of Pseudomonas Solanacearum in Sympton-latent Seedings of Sweet Potato

Six hybridoma cell lines secreting monoclonal antibodies (McAbs) against the pathogen of Pseudomonas solanacearum from sweet potato have been produced.The McAbs showed positive reactions to 7 sweet-potato-infected strains,and negative to other bacteria of Pseudomonas and genus else,and various bacteria infected seedings of sweet potato detected in the test.The ELISA liters of the culture supernatanls and the ascites antibodies were respectively 102-103 and 105-106.The sensitivity of antigen detected was 11.5μg/ml (protein concentration).The original characters of the hybridoma cell and culture supernatants antibodies could be kept over three years.Six antibodies showed positive to all of 3 pathogenicity types of Pseudomonas solanacearum from sweet potato in Fujian province.The inoculated samples could be detected out by the indirect ELISA at the rate of 100%.In 119 portions of symptonlatent seedlings collected from field cases,51 and 68 were respectively positive and negative.To compare with the antibody and inoculation detection,the result showed the sensitivity and specificity of antibody detection were more height than that of inoculation detection,and more rapid and handy.