Effects of applied microbial cultures on the soil bacteria community structure of <i>Phyllostachys edulis</i>

YUAN Zongsheng

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YUAN Z S. Effects of applied microbial cultures on the soil bacteria community structure of Phyllostachys edulis [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−11

Citation:

YUAN Z S. Effects of applied microbial cultures on the soil bacteria community structure of Phyllostachys edulis [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−11

YUAN Z S. Effects of applied microbial cultures on the soil bacteria community structure of Phyllostachys edulis [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−11

Citation:

YUAN Z S. Effects of applied microbial cultures on the soil bacteria community structure of Phyllostachys edulis [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−11

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Effects of applied microbial cultures on the soil bacteria community structure of Phyllostachys edulis

YUAN Zongsheng

College of Geography and Oceanography, Minjiang University, Fuzhou, Fujian　350108

Received Date: 2023-08-17
Rev Recd Date: 2023-11-13

Available Online: 2024-06-26

Abstract

Abstract

: Objective To explore the effects of microbial cultures（Enterobacter aerogenes CT-B09-2、Bacillus amyloliquefaciens JL-B06 and Acinetobacter calcoaceticus WYS-A01-1）on the structure and diversity of Phyllostachys edulis soil bacterial communities. Method Taking P. edulis seedlings as the research object, compound microbial cultures were applied by root irrigation. 30 days after the application, rhizosphere and non-rhizosphere soil samples of P. edulis seedlings were collected to measure soil physical and chemical properties. Total soil DNA was extracted and 16S rRNA high-throughput sequencing was performed to analyze the impact of compound microbial cultures on soil bacterial community structure and diversity. Results The compound microbial cultures can effectively increase the content of available phosphorus in soil, regulate soil pH, and improved the functional activities related to material metabolism and decomposition of carbon compounds in rhizosphere soil. 26 phyla, 65 classes, 158 orders, 253 families, 448 genera, and 674 species were detected in P. edulis rhizosphere and non-rhizosphere soil. The main dominant bacterial phyla are Proteobacteria, Firmicutes, Actinobacteriota, Acidobacteriota, and Chloroflexi. After the application of compound microbial cultures, the number of species in the rhizosphere soil microbial community of P. edulis increased significantly, but there was no significant change in non-rhizosphere soil samples. Conclusion The compound microbial cultures can regulate soil mineral elements, improve soil pH, and regulate the composition of bacterial microbial community.
- microbial cultures,
- Phyllostachys edulis,
- soil bacterial community,
- diversity

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References(30)

References

[1]	ZHU J, LI M, WHELAN M. Phosphorus activators contribute to legacy phosphorus availability in agricultural soils: A review [J]. The Science of the Total Environment, 2018, 612: 522−537. doi: 10.1016/j.scitotenv.2017.08.095
[2]	李玉敏, 冯鹏飞. 基于第九次全国森林资源清查的中国竹资源分析 [J]. 世界竹藤通讯, 2019, 17(6):45−48. LI Y M, FENG P F. Bamboo resources in China based on the ninth national forest inventory data [J]. World Bamboo and Rattan, 2019, 17(6): 45−48. (in Chinese)
[3]	张雨佳, 刘丽, 邹龙海, 等. 毛竹PheFT12a基因过表达对拟南芥开花及芽发育的影响 [J]. 核农学报, 2023, 37(11):2142−2150. doi: 10.11869/j.issn.1000-8551.2023.11.2142 ZHANG Y J, LIU L, ZOU L H, et al. Effects of overexpression of the moso bamboo PheFT12a on flowering and bud development of Arabidopsis [J]. Journal of Nuclear Agricultural Sciences, 2023, 37(11): 2142−2150. (in Chinese) doi: 10.11869/j.issn.1000-8551.2023.11.2142
[4]	刘雅娜, 李袁凯, 王金莲, 等. 不同微生物菌剂对马铃薯的促生作用研究 [J]. 干旱区资源与环境, 2023, 37(9):136−143. LIU Y N, LI Y K, WANG J L, et al. Effects of different microbial agents on the growth of potato [J]. Journal of Arid Land Resources and Environment, 2023, 37(9): 136−143. (in Chinese)
[5]	徐冰石, 陈伏生, 张林平, 等. 土壤产气肠杆菌的解磷特性及其对毛竹苗的促生作用 [J]. 林业科学研究, 2022, 35(3):38−46. XU B S, CHEN F S, ZHANG L P, et al. Phosphate solubilizing characteristics of Enterobacter aerogenes and its growth-promoting effect on Phyllostachys edulis seedlings [J]. Forest Research, 2022, 35(3): 38−46. (in Chinese)
[6]	安福涛, 刘欣宇, 孙富余. 3种芽孢杆菌在番茄生产过程中的应用效果研究 [J]. 山西农业大学学报(自然科学版), 2023, 43(1):82−88. AN F T, LIU X Y, SUN F Y. Study on the effectiveness of three Bacillus strains in tomato production [J]. Journal of Shanxi Agricultural University (Natural Science Edition), 2023, 43(1): 82−88. (in Chinese)
[7]	蓝江林, 朱育菁, 刘波, 等. 茄子3株内生细菌分别与青枯雷尔氏菌混合培养特性研究 [J]. 福建农业学报, 2012, 27(10):1087−1092. doi: 10.3969/j.issn.1008-0384.2012.10.011 LAN J L, ZHU Y J, LIU B, et al. Populations of co-cultivated endophytic bacteria from eggplants and Ralstonia solanacearum [J]. Fujian Journal of Agricultural Sciences, 2012, 27(10): 1087−1092. (in Chinese) doi: 10.3969/j.issn.1008-0384.2012.10.011
[8]	CHANDRAKALA C, VOLETI S R, BANDEPPA S, et al. Silicate solubilization and plant growth promoting potential of Rhizobium sp. isolated from rice rhizosphere [J]. Silicon, 2019, 11(6): 2895−2906. doi: 10.1007/s12633-019-0079-2
[9]	武杞蔓, 刘朋宇, 张颖, 等. 微生物菌肥对番茄生长、品质及糖代谢相关酶的影响 [J]. 江苏农业科学, 2022, 50(24):125−130. WU Q M, LIU P Y, ZHANG Y, et al. Influences of microbial agents on tomato growth, quality and enzymes related to sugar metabolism [J]. Jiangsu Agricultural Sciences, 2022, 50(24): 125−130. (in Chinese)
[10]	万小琪, 窦维卉, 杨雪, 等. 不同农艺型措施对温室黄瓜连作土壤的改良效果 [J]. 江苏农业科学, 2022, 50(23):228−234. WAN X Q, DOU W H, YANG X, et al. Effects of different agronomic measures on continuous cropping soil improvement of cucumber in greenhouse [J]. Jiangsu Agricultural Sciences, 2022, 50(23): 228−234. (in Chinese)
[11]	张建鹏. 化肥减量配施微生物菌肥及土壤调理剂对重茬马铃薯生长发育和土壤质量的影响 [J]. 江苏农业科学, 2023, 51(7):205−212. ZHANG J P. Influences of chemical fertilizer reduction combined with microbial fertilizer and soil conditioner on growth and soil quality of continuous potatoes [J]. Jiangsu Agricultural Sciences, 2023, 51(7): 205−212. (in Chinese)
[12]	袁宗胜. 毛竹根区土壤与内生细菌多样性及促生细菌筛选[D]. 福州: 福建农林大学, 2015. YUAN Z S. The Diversity of root zone soil bacteria and endophytic bacteria of moso bamboo and screening of growth-promoting endophytic bacteria[D]. Fuzhou: Fujian Agriculture and Forestry University, 2015. (in Chinese)
[13]	国家林业局发布. 中华人民共和国林业行业标准-森林土壤分析方法[M]. 北京: 中国标准出版社, 2000.
[14]	SCHLOSS P D, WESTCOTT S L, RYABIN T, et al. Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities [J]. Applied and Environmental Microbiology, 2009, 75(23): 7537−7541. doi: 10.1128/AEM.01541-09
[15]	张旋, 杜薇, 徐颖, 等. 包头市半干旱型森林公园土壤细菌多样性与功能 [J]. 生物多样性, 2022, 30(7):193−204. doi: 10.17520/biods.2022245 ZHANG X, DU W, XU Y, et al. Soil bacterial diversity and function in semi-arid forest parks in Baotou City [J]. Biodiversity Science, 2022, 30(7): 193−204. (in Chinese) doi: 10.17520/biods.2022245
[16]	LOUCA S, PARFREY L W, DOEBELI M. Decoupling function and taxonomy in the global ocean microbiome [J]. Science, 2016, 353(6305): 1272−1277. doi: 10.1126/science.aaf4507
[17]	涂保华, 符菁, 赵远, 等. 基于光合菌剂的复合微生物菌肥对土壤速效养分含量及微生物群落结构多样性的影响 [J]. 西南农业学报, 2019, 32(12):2878−2884. TU B H, FU J, ZHAO Y, et al. Effects of photosynthetic fungicide based compound microbial fertilizer on soil available nutrient content and microbial community structure diversity [J]. Southwest China Journal of Agricultural Sciences, 2019, 32(12): 2878−2884. (in Chinese)
[18]	张明, 陈泓志, 李明. 一种复合微生物菌肥对穿心莲生长、品质及土壤性质的影响 [J]. 中国实验方剂学杂志, 2023, 29(4):153−160. ZHANG M, CHEN H Z, LI M. Effect of a compound microbial fertilizer on growth, quality, and soil properties of Andrographis paniculata [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2023, 29(4): 153−160. (in Chinese)
[19]	袁雅文. 有益微生物作用机理及微生物菌肥的应用前景 [J]. 杂交水稻, 2022, 37(4):7−14. YUAN Y W. Action mechanism of beneficial microorganisms and application prospect of microbial fertilizers [J]. Hybrid Rice, 2022, 37(4): 7−14. (in Chinese)
[20]	决超. 微生物菌肥与土壤改良基质对连作马铃薯土壤性质及微生物群落的影响 [J]. 江苏农业科学, 2023, 51(1):218−224. JUE C. Effects of microbial fertilizer and soil improvement substrate on soil properties and microbial community of continuous cropping potato [J]. Jiangsu Agricultural Sciences, 2023, 51(1): 218−224. (in Chinese)
[21]	高志伟, 刘凡惠, 贾美清, 等. 基于Illumina高通量测序的天津北大港湿地沉积物细菌群落特征和多样性分析 [J]. 天津师范大学学报(自然科学版), 2021, 41(4):45−52. GAO Z W, LIU F H, JIA M Q, et al. Illumina-based high-throughput sequencing analysis of bacterial community structure and diversity in Beidagang wetland sediment, Tianjin [J]. Journal of Tianjin Normal University (Natural Science Edition), 2021, 41(4): 45−52. (in Chinese)
[22]	PIOUCEAU J, BOIS G, PANFILI F, et al. Effects of high nutrient supply on the growth of seven bamboo species [J]. International Journal of Phytoremediation, 2014, 16(7/8/9/10/11/12): 1042−1057.
[23]	LUO X Z, KEENAN T F, CHEN J M, et al. Global variation in the fraction of leaf nitrogen allocated to photosynthesis [J]. Nature Communications, 2021, 12(1): 4866. doi: 10.1038/s41467-021-25163-9
[24]	冼康华, 苏江, 付传明, 等. 不同菌肥对华重楼根际土壤微生物多样性及理化性质的影响 [J]. 广西科学, 2021, 28(6):616−625. XIAN K H, SU J, FU C M, et al. Effects of different bacterial fertilizers on microbial diversity, physical and chemical properties of rhizosphere soil of Paris polyphylla var. chinensis [J]. Guangxi Sciences, 2021, 28(6): 616−625. (in Chinese)
[25]	胡基华, 李晶, 张淑梅, 等. 解淀粉芽孢杆菌TF28对设施连作黄瓜根际土壤酶活性和微生物的调节 [J]. 江苏农业科学, 2020, 48(7):152−156. HU J H, LI J, ZHANG S M, et al. Regulation of Bacillus amyloliquefaciens TF28 on soil enzyme activities and microorganisms in rhizosphere of cucumber under continuous cropping in greenhouse [J]. Jiangsu Agricultural Sciences, 2020, 48(7): 152−156. (in Chinese)
[26]	张昕, 张立钦, 林海萍, 等. 引入黄瓜根围的2株生防菌株的生态效应 [J]. 浙江林学院学报, 2007, 24(6):649−653. ZHANG X, ZHANG L Q, LIN H P, et al. Biocontrol agents introduced into a Cucumis melo rhizosphere [J]. Journal of Zhejiang Forestry College, 2007, 24(6): 649−653. (in Chinese)
[27]	VAN DEYNZE A, ZAMORA P, DELAUX P M, et al. Nitrogen fixation in a Landrace of maize is supported by a mucilage-associated diazotrophic microbiota [J]. PLoS Biology, 2018, 16(8): e2006352. doi: 10.1371/journal.pbio.2006352
[28]	IGIEHON N O, BABALOLA O O. Rhizosphere microbiome modulators: Contributions of nitrogen fixing bacteria towards sustainable agriculture [J]. International Journal of Environmental Research and Public Health, 2018, 15(4): 574. doi: 10.3390/ijerph15040574
[29]	SÁNCHEZ-NAVARRO V, ZORNOZA R, FAZ Á, et al. Inoculation with different nitrogen-fixing bacteria and arbuscular mycorrhiza affects grain protein content and nodule bacterial communities of a fava bean crop [J]. Agronomy, 2020, 10(6): 768. doi: 10.3390/agronomy10060768
[30]	刘耀辉, 盛可银, 罗建荣, 等. 溶磷菌混施对土壤微生物群落及毛竹生长的影响 [J]. 江西农业大学学报, 2023, 45(2):298−310. LIU Y H, SHENG K Y, LUO J R, et al. Effects of phosphorus solubilizing bacterial compound suspensions on growth of moso bamboo(Phyllostachys edulis) and soil microbial community structures [J]. Acta Agriculturae Universitatis Jiangxiensis, 2023, 45(2): 298−310. (in Chinese)