Transient Expression of Gus Genes Controlled by Differential Regulator Sequences in Citrus Protoplasts

Wang Feng; Zhu Zhen; Li Xianghui; Zhang Wencai

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Wang Feng, Zhu Zhen, Li Xianghui, Zhang Wencai. Transient Expression of Gus Genes Controlled by Differential Regulator Sequences in Citrus Protoplasts[J]. Fujian Journal of Agricultural Sciences, 1998, 13(2): 1-5.

Citation:

Wang Feng, Zhu Zhen, Li Xianghui, Zhang Wencai. Transient Expression of Gus Genes Controlled by Differential Regulator Sequences in Citrus Protoplasts[J]. Fujian Journal of Agricultural Sciences, 1998, 13(2): 1-5.

Citation:

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Transient Expression of Gus Genes Controlled by Differential Regulator Sequences in Citrus Protoplasts

1.
Key Laboratory of Genetic Engineering of Fujian Province, Fujian Academy of Agricultural Sciences, Fuzhou 350013;
2.
Institute of Genetics, Academia Sinica;
3.
Citrus Research Institute Huazhong Agricultural University

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Received Date: 1996-05-09
Rev Recd Date: 1998-03-03
Publish Date: 1998-06-15

Abstract

Abstract

Gus genes controlled by several kinds of regulator sequences were introduced into citrus protoplasts respectively by PEG and lipofectin mediated transformation.The transient expression of gus gene in citrus protoplasts had been investigated.The expression level of the citrus protoplasts transformated with plasimid pBI121 and p35SGSINT (both gus genes were controlled by CaMV35S promoter) was higher than that transformated with plasmid pAT13 (gus gene controlled by Adhl promoter) and pRGN73 (gus gene under control of rice rbcs promoter).It was found that intron increase the expression level in citrus protoplasts.
- Transient expression,
- Gus gene,
- Regulator sequenece,
- Citrus,
- Protoplasts

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References(6)

References

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[7] Fromm M F, Taylor L P, et al.Expression of genest ransfered intomonocot and dicot plant cells by electroporation Proc.N et al.Acad.Sci.USA, 1985, 82:5842-5848

[9] Jefferson R A, K avanag h T A, et al.Gusfusions:β-glucu ronidaseas a senstive and versatile genefusion marker in higher plants.EMBO J., 1987, 6:3901-3907

[14] Cocking E C, Peberdy J F,(eds.).The use of protoplasts from fungi and higher plants as genetic systems.Dept.Bot any, Univ.Notting ham.1974

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