Isolation, Identification, and Culture characteristics of a Genotype Ⅱ Goose Astrovirus

LAN Qin; LIU Rongchang; ZHANG Jiaxue; CHEN Wei; JIAO Wenlong; LIANG Qizhang; JIANG Nansong; WAN Chunhe; CHENG Longfei; CHEN Hongmei; FU Qiuling; FU Guanghua; HUANG Yu

doi:10.19303/j.issn.1008-0384.2024.04.002

Volume 39 Issue 4

Apr. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(4): 378-386

LAN Q, LIU R C, ZHANG J X, et al. Isolation, Identification, and Culture characteristics of a Genotype Ⅱ Goose Astrovirus [J]. Fujian Journal of Agricultural Sciences，2024，39（4）：378−386 doi: 10.19303/j.issn.1008-0384.2024.04.002

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LAN Q, LIU R C, ZHANG J X, et al. Isolation, Identification, and Culture characteristics of a Genotype Ⅱ Goose Astrovirus [J]. Fujian Journal of Agricultural Sciences，2024，39（4）：378−386 doi: 10.19303/j.issn.1008-0384.2024.04.002

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Isolation, Identification, and Culture characteristics of a Genotype Ⅱ Goose Astrovirus

doi: 10.19303/j.issn.1008-0384.2024.04.002

LAN Qin^{1, 2
,},
LIU Rongchang^{2, 3
,},
ZHANG Jiaxue^{1, 2},
CHEN Wei^{1, 2},
JIAO Wenlong^{1, 2},
LIANG Qizhang^{2, 3},
JIANG Nansong^{2, 3},
WAN Chunhe^{2, 3},
CHENG Longfei^{2, 3},
CHEN Hongmei^{2, 3},
FU Qiuling^{2, 3},
FU Guanghua^{2, 3},
HUANG Yu^{1, 2, 3
,
,}

1.
College of Animal Sciences (College of Bee science), Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
3.
Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian　350013, China

Received Date: 2024-02-20
Rev Recd Date: 2024-03-10

Available Online: 2024-06-26

Publish Date: 2024-04-28

Abstract

Abstract

Objective To clarify the pathogenicity and culture characteristics of Goose astrovirus(GAstV). Methods Disease diagnosis and pathogen isolation, identification, gene sequencing, culture characteristics , and pathogenicity on a goose at a farm in Guangdong showing visceral and gout symptoms were conducted. Results The Goose astrovirus (GAstV) isolated from the tissue sample was named GD2208. It showed the highest homology of 97.4%–99.1% with the reference strain GAstV-Ⅱ based on the ORF2 gene nucleotide sequence. They belonged to the same evolutionary branch on the evolutionary tree. In an animal regression test, the chicks inoculated with GD2208 displayed similar clinical symptoms as well as the pathological and histopathological changes observed on the naturally infected 3-day-old goose and had a 20% mortality rate. The isolate could proliferate in goose embryos, but unable to adapt to SPF chicken or Muscovy duck embryos after 4 generations of blind transmission. It could also grow in GEK and LMH cells with the apparent cytopathic effects or in DF-1 cells without the effects after 8 generations of blind transmission. Conclusion A GAstV-Ⅱ strain isolated from a diseased goose when inoculated in healthy chicks produced similar symptoms on the birds, and it could grow in goose embryos, GEK, LMH, and DF-1 cells.
- Goose astrovirus,
- isolation,
- identification,
- cultural characteristics

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References(33)

References

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