Detection and Field Monitoring of Water Spinach Canker

CHEN Siyan; XUE Yang; HAN Haiya; LIN Minyi; LI Xuyu; YIN Deming; TAO Jie; LI Shimao; PENG Liya; XI Zhuojun; ZHOU Jianuan

doi:10.19303/j.issn.1008-0384.2023.03.010

Volume 38 Issue 3

Mar. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(3): 339-345

CHEN S Y, XUE Y, HAN H Y, et al. Detection and Field Monitoring of Water Spinach Canker [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：339−345 doi: 10.19303/j.issn.1008-0384.2023.03.010

Citation:

CHEN S Y, XUE Y, HAN H Y, et al. Detection and Field Monitoring of Water Spinach Canker [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：339−345 doi: 10.19303/j.issn.1008-0384.2023.03.010

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Detection and Field Monitoring of Water Spinach Canker

doi: 10.19303/j.issn.1008-0384.2023.03.010

CHEN Siyan^1
,,
XUE Yang^1
,,
HAN Haiya²,
LIN Minyi³,
LI Xuyu⁴,
YIN Deming⁵,
TAO Jie⁶,
LI Shimao⁷,
PENG Liya⁸,
XI Zhuojun²,
ZHOU Jianuan^{1
,
,}

1.
National Key Laboratory of Green Pesticide/Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Integrative Microbiology Research Center, South China Agricultural University, Guangzhou, Guangdong　510642, China
2.
Dongguan Agricultural Technology Extension Administration office, Dongguan, Guangdong　523010, China
3.
Agriculture and Forestry Irrigation Bureau of Xiegang Town, Dongguan, Guangdong　523590, China
4.
Agricultural Technology Service Center of Dalingshan Town, Dongguan, Guangdong　523820, China
5.
Agricultural Technology Service Center of Gaobu Town, Dongguan, Guangdong　523270, China
6.
Agricultural Technology Service Center of Mayong Town, Dongguan, Guangdong　523133, China
7.
Agricultural Technology Service Center of Daojiao Town, Dongguan,Guangdong　523170, China
8.
Shenzhen Agricultural Science and Technology Promotion Center, Shenzhen, Guangdong　518101, China

Received Date: 2022-11-19
Rev Recd Date: 2022-12-26

Available Online: 2023-03-28

Publish Date: 2023-03-28

Abstract

Abstract

Objective An efficient and rapid detection method was developed to monitor the spread of a newly discovered bacterial canker disease infected by Xanthomonas perforans that seriously impacted the production of water spinach (Ipomoea aquatica), a recently introduced and increasingly important cash crop in southern China. Method Two pairs of primers were designed to detect X. perforans based on the specific sequences in the genomes including TC2-1_002562 and TC2-1_002580, which encode a phage terminator large subunit family protein and a phage family protein, respectively. The applicability of the proposed methodology with a single PCR reaction was verified. Result The newly developed PCR method exhibited a high specificity for detecting X. perforans. In the first sampling batch of water spinach plants, planting soils, and water collected from regions in Guangdong Province, the pathogen was only detected in 5 specimens of water spinach leaves from Dongguan. However, during the subsequent second sampling 10 days later, the pathogen was found in numerous specimens from all sources. Conclusion The established PCR method could rapidly detect X. perforans for early disease diagnosis on water spinach. As the field monitoring indicated, the endemic was prevalent in Dongguan and Shenzhen, possibly some other localities, but spreading fast. The origin of the infection might come from the stored water spinach seedlings and/or seeds. The area water and soil could also link to the spread of the disease. Urgent study to timely develop effective prevention and control measures is in order.
- Water spinach,
- canker,
- Xanthomonas perforans,
- PCR detection

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References(14)

References

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