Optimized Proteolysis of Chicken Protein for Food Flavoring

YU Ya-hui; CHEN Qin-wen; LI Xiao-ting; FANG Ting

doi:10.19303/j.issn.1008-0384.2020.01.012

Volume 35 Issue 1

Jan. 2020

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Fujian Journal of Agricultural Sciences > 2020 > 35(1): 80-89. > DOI: 10.19303/j.issn.1008-0384.2020.01.012

YU Y H, CHEN Q W, LI X T, et al. Optimized Proteolysis of Chicken Protein for Food Flavoring [J]. Fujian Journal of Agricultural Sciences，2020，35（1）：80−89. DOI: 10.19303/j.issn.1008-0384.2020.01.012

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Optimized Proteolysis of Chicken Protein for Food Flavoring

College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

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Received Date: September 21, 2019
Revised Date: October 24, 2019

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Abstract

Abstract

Objective The stepwise enzymatic hydrolysis was applied to hydrolyze chicken protein for the preparation of a food flavoring ingredient with improved quality.
Method An animal and a compound proteolytic enzyme were used to digest the chicken protein. Free amino acid and peptide contents in the hydrolyzed material were employed as the criteria to initially identify the critical process evaluation parameters based on a single factor Plackett-Burman design. A steepest ascent test was conducted to approximate the maximum response area and arrive at the center point in a response surface experiment. Then, according to the selected indicators for the Box-Behnken experiment, optimized process conditions were determined.
Result The selected indicators for the Box-Behnken optimization included the free amino acid and polypeptide contents as well as the flavor scores on the thermal reaction products of the hydrolyzed compounds. The optimal processing conditions were found to include the addition of 0.82% enzymes on the chicken protein substrate, a solid-liquid ratio of 1 ∶ 4.40, and a reaction duration of 7.26 h to achieve an actual composite evaluation score of 88.97 in comparison to the predicted 89.41. The method was deemed reliable.
Conclusion The multi-indicator response surface method effectively optimized the enzymatic proteolysis process of chicken protein materials in producing a high quality flavoring ingredient for food applications.
- enzymatic proteolysis,
- stepwise hydrolysis,
- Plackett-Burman design,
- response surface optimization

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[1]	孙颖, 张莉莉, 张玉玉, 等. 3种酶解液中游离氨基酸的分析 [J]. 精细化工, 2018, 35(6):1004−1008, 1014. SUN Y, ZHANG L L, ZHANG Y Y, et al. Analysis of free amino acids in three kinds of enzymatic hydrolysates [J]. Fine Chemicals, 2018, 35(6): 1004−1008, 1014.(in Chinese)
[2]	何蓉蓉, 李怡芳, 李维熙, 等. 鸡汤健康功能的研究现状 [J]. 食品与生物技术学报, 2012, 31(1):17−27. DOI: 10.3969/j.issn.1673-1689.2012.01.003 HE R R, LI Y F, LI W X, et al. Review on health functions of chicken broths [J]. Journal of Food Science and Biotechnology, 2012, 31(1): 17−27.(in Chinese) DOI: 10.3969/j.issn.1673-1689.2012.01.003
[3]	ZHUANG M Z, LIN L Z, ZHAO M M, et al. Sequence, taste and umami-enhancing effect of the peptides separated from soy sauce [J]. Food Chemistry, 2016, 206: 174−181. DOI: 10.1016/j.foodchem.2016.03.058
[4]	SONG H L, XIA L J. Aroma extract dilution analysis of a beef flavouring prepared from flavour precursors and enzymatically hydrolysed beef [J]. Flavour and Fragrance Journal, 2008, 23(3): 185−193. DOI: 10.1002/ffj.1873
[5]	陈海涛, 徐晓兰, 张宁, 等. 鸡肉酶解工艺对热反应鸡肉香精香气的影响 [J]. 食品科学, 2013, 34(9):150−154. DOI: 10.7506/spkx1002-6630-201309031 CHEN H T, XU X L, ZHANG N, et al. Optimal chicken enzymatic hydrolysate for the preparation of chicken flavoring [J]. Food Science, 2013, 34(9): 150−154.(in Chinese) DOI: 10.7506/spkx1002-6630-201309031
[6]	张永生, 耿伟涛, 江方, 等. 水解度对热反应鸡肉香精呈味的影响 [J]. 食品科技, 2017, 42(12):270−275. ZHANG Y S, GENG W T, JIANG F, et al. Effect of degree of hydrolysis on the taste characteristic of chicken flavor [J]. Food Science and Technology, 2017, 42(12): 270−275.(in Chinese)
[7]	肖作兵, 吴旻玲, 牛云蔚. 双指标响应面优化鸡肉酶解工艺 [J]. 中国食品学报, 2016, 16(11):121−128. XIAO Z B, WU M L, NIU Y W. Optimization of enzymatic hydrolysis process by dual indicators response surface analysis [J]. Journal of Chinese Institute of Food Science and Technology, 2016, 16(11): 121−128.(in Chinese)
[8]	邱燕翔, 陆志鸿, 许守庆, 等. 鸡肉蛋白酶解工艺及水解产物成分研究 [J]. 食品科技, 2017, 42(3):284−287. QIU Y X, LU Z H, XU S Q, et al. Optimum technical conditions of chicken hydrolysis and composition of hydrolysate [J]. Food Science and Technology, 2017, 42(3): 284−287.(in Chinese)
[9]	侯佰慧, 夏杨毅, 周涛, 等. 鸡肉酶解液制备鸡汤热反应过程中呈味物质的变化 [J]. 食品科学, 2017, 38(14):175−180. DOI: 10.7506/spkx1002-6630-201714027 HOU B H, XIA Y Y, ZHOU T, et al. Change of taste components in thermal reaction model system containing chicken enzymatic hydrolysate with reaction time [J]. Food Science, 2017, 38(14): 175−180.(in Chinese) DOI: 10.7506/spkx1002-6630-201714027
[10]	吴晖, 任尧, 李晓凤. 单酶和双酶分步酶解鸭蛋清蛋白的工艺 [J]. 食品与发酵工业, 2010, 36(11):93−97. WU H, REN Y, LI X F. Preparation of bioactive peptides via hydrolysis of duck egg-white protein by single/binary enzymes [J]. Food and Fermentation Industries, 2010, 36(11): 93−97.(in Chinese)
[11]	CAI M H, ZHOU X S, SUN X Q, et al. Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus [J]. Journal of Industrial Microbiology & Biotechnology, 2009, 36(3): 381−389.
[12]	侯晓慧, 张金泽, 林静, 等. 植物源α-低聚半乳糖制备工艺条件优化 [J]. 食品与发酵工业, 2014, 40(1):119−123. HOU X H, ZHANG J Z, LIN J, et al. Response surface methodology for preparation α-galactooligosaccharide from plants by fermentation [J]. Food and Fermentation Industries, 2014, 40(1): 119−123.(in Chinese)
[13]	刘雪飞, 郭端煦, 吕淑霞, 等. 莫海威芽孢杆菌J7产抑菌物质培养基发酵优化 [J]. 中国食品学报, 2018, 18(10):109−119. LIU X F, GUO D X, LÜ S X, et al. Fermentation optimization of culture medium for antibacterial material production of Bacillus mojavensis J7 [J]. Journal of Chinese Institute of Food Science and Technology, 2018, 18(10): 109−119.(in Chinese)
[14]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. GB/T 8314-2013, 茶游离氨基酸总量的测定[S]. 2013.
[15]	鲁伟, 任国谱, 宋俊梅. 蛋白水解液中多肽含量的测定方法 [J]. 食品科学, 2005, 26(7):169−171. DOI: 10.3321/j.issn:1002-6630.2005.07.039 LU W, REN G P, SONG J M. Determination of content of peptides in protein hydrolysates [J]. Food Science, 2005, 26(7): 169−171.(in Chinese) DOI: 10.3321/j.issn:1002-6630.2005.07.039
[16]	徐向宏, 何明珠. 试验设计与Design-Expert、SPSS应用[M]. 北京: 科学出版社, 2010.
[17]	朱建平, 胡朝霞, 王艺明. 高级计量经济学导论[M]. 北京: 北京大学出版社, 2009: 73-78.
[18]	周涛. 热反应鸡汤呈味物质变化研究[D]. 重庆: 西南大学, 2016. ZHOU T. Study on the change of taste components in thermal-reaction chicken soup[D]. Chongqing: Southwest University, 2016. (in Chinese)
[19]	LIU Y P, ZHENG P, SUN Z H, et al. Economical succinic acid production from cane molasses by Actinobacillus succinogenes [J]. Bioresource Technology, 2008, 99(6): 1736−1742. DOI: 10.1016/j.biortech.2007.03.044
[20]	梁昌聪, 刘磊, 张建华, 等. 响应面法优化绿色木霉H06产孢培养条件 [J]. 广东农业科学, 2014, 41(19):80−85. DOI: 10.3969/j.issn.1004-874X.2014.19.019 LIANG C C, LIU L, ZHANG J H, et al. Optimization of fermentation conditions for sporulation quantity by Trichoderma viride strain H06 using response surface methodology [J]. Guangdong Agricultural Sciences, 2014, 41(19): 80−85.(in Chinese) DOI: 10.3969/j.issn.1004-874X.2014.19.019
[21]	安攀宇, 赵珊, 张淼, 等. 响应面法优化鸡胸肉蛋白酶解工艺 [J]. 肉类研究, 2018, 32(11):22−29. AN P Y, ZHAO S, ZHANG M, et al. Optimization of enzymatic hydrolysis of chicken breast proteins by response surface methodology [J]. Meat Research, 2018, 32(11): 22−29.(in Chinese)

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