Optimization of Seed Sterilization and Rooting Medium for Regeneration of <i>Brassica napus</i>

FU Shu; LIU Zhao-xia; CHEN Jin-zhi; SUN Geng-xiao; SUN Cui-ying; CHEN Jin; YANG Guang

doi:10.19303/j.issn.1008-0384.2019.12.003

Volume 34 Issue 12

Dec. 2019

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Fujian Journal of Agricultural Sciences > 2019 > 34(12): 1371-1378. > DOI: 10.19303/j.issn.1008-0384.2019.12.003

FU S, LIU Z X, CHEN J Z, et al. Optimization of Seed Sterilization and Rooting Medium for Regeneration of Brassica napus [J]. Fujian Journal of Agricultural Sciences，2019，34（12）：1371−1378.. DOI: 10.19303/j.issn.1008-0384.2019.12.003

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Optimization of Seed Sterilization and Rooting Medium for Regeneration of Brassica napus

FU Shu^{1, 2, 3,},
LIU Zhao-xia^{1, 2, 3},
CHEN Jin-zhi^{1, 2, 3},
SUN Geng-xiao^{1, 2, 3},
SUN Cui-ying^{1, 2, 3},
CHEN Jin^{1, 2, 3},
YANG Guang^{1, 2, 3, ,}

1.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/ Institute of Applied Ecology, Fuzhou, Fujian　350002, China
2.
Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, Fujian　350002, China
3.
Key Laboratory of Green Control of Insect Pests (Fujian Province University), Fuzhou, Fujian　350002, China

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Received Date: November 17, 2019
Revised Date: December 08, 2019

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Abstract

Abstract

Objective Seed sterilization and rooting culture are important steps in plant tissue culture. At present, there is considerable variation in the concentration of sodium hypochlorite (NaClO) and seed sterilization time of Brassica napus, as well as the type and concentration of hormone in the rooting media. The purpose of this study was to optimize the method of seed sterilization with NaClO and the formula of rooting medium for B. napus regeneration.
Method The optimum concentration of NaClO for seed sterilization was screened by comparing the growth status (such as germination rate, healthy shoot rate and rate of colonies) of seeds treated with different concentrations (0.1%–30.0%) of NaClO, and the appropriate sterilization time was optimized based on the growth status of seeds treated with the optimal concentration of NaClO for 10—30 min. Moreover, the optimum formula of rooting media was screened by comparing the rooting regeneration of seedlings in the media containing 0.1–2.0 mg·L⁻¹ naphthylacetic acid (NAA) / indolebutyric acid (IBA).
Result The optimum concentration of NaClO for seed sterilization was 2.0% or 3.0%, and the best time for seed sterilization with 3.0% NaClO was 15 min, causing the 97.4% of germination rate and 85.9% of healthy shoot rate with least rate of colonies. Moreover, this method of seed sterilization is applicable to other 9 plants, especially for B. napus, B. pekinensis and Arabidopsis thaliana. The root regenerated best in the rooting medium containing 0.1 mg·L⁻¹ NAA, in which 92.5% of root regenerated from the cutting point on the 7^th day with the most lateral roots.
Conclusion This study optimized the method of seed sterilization and the formula of rooting medium, which laid a foundation for efficient plant tissue culture of B. napus.
- Brassica napus,
- seed sterilization,
- sodium hypochlorite,
- rooting,
- NAA,
- IBA

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Optimization of Seed Sterilization and Rooting Medium for Regeneration of Brassica napus

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