Identification of CIPK Family in Rice and qRT-PCR Analysis on OsCIPK5 Induced by Magnaporthe oryzae

YU Si-si; LUO Xi; LIAN Ling; XU Hui-bin; CHEN Li-ping; WEI Yi-dong; CAI Qiu-hua; XIE Hua-an; ZHANG Jian-fu

doi:10.19303/j.issn.1008-0384.2019.11.001

Volume 34 Issue 11

Nov. 2019

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Fujian Journal of Agricultural Sciences > 2019 > 34(11): 1237-1245. > DOI: 10.19303/j.issn.1008-0384.2019.11.001

YU S S, LUO X, LIAN L, et al. Identification of CIPK Family in Rice and qRT-PCR Analysis on OsCIPK5 Induced by Magnaporthe oryzae [J]. Fujian Journal of Agricultural Sciences，2019，34（11）：1237−1245.. DOI: 10.19303/j.issn.1008-0384.2019.11.001

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Identification of CIPK Family in Rice and qRT-PCR Analysis on OsCIPK5 Induced by Magnaporthe oryzae

YU Si-si^{1, 2, 3,},
LUO Xi^{2, 3},
LIAN Ling^{2, 3},
XU Hui-bin^{2, 3},
CHEN Li-ping^{2, 3},
WEI Yi-dong^{2, 3},
CAI Qiu-hua^{2, 3},
XIE Hua-an^{1, 2, 3, ,},
ZHANG Jian-fu^{1, 2, 3, ,}

1.
College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350019, China
3.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/Fuzhou Branch, National Rice Improvement Center/Incubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology/Base of South-China, National Key Laboratory of Hybrid Rice/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/ National Engineering Laboratory of Rice, Fuzhou, Fujian　350003, China

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Received Date: October 04, 2019
Revised Date: November 01, 2019

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Abstract

Abstract

Objective The family of calcineurin B-like protein-interacting protein kinase genes (CIPKs) in rice was studied using the latest sequencing data to further understand the signal transduction involving a variety of kinases in plants in respond to environmental changes.
Methods The structures of CIPKs in Arabidopsis thaliana and rice were analyzed. Combining the bioinformatics and qRT-PCR technology, expressions of the CIPK familie and that of the genes induced by M. grisea in Japonica rice cv. 'Yunyin' were compared.
Results A total of 31 OsCIPK genes were identified in the rice genome databank. The phylogenetic tree analysis showed that these 31 OsCIPKs could be divided into 5 subfamilies, which had different structural characteristics of exon-intron and UTR. The expression of OsCIPK5, which was selected from a trend cluster of the gene expressiong profile of 'Yunyin' induced by M. grisea, could be induced by M. grisea.
Conclusions It appeared that the intron deletion and fragment duplication played an important role in the expansion of OsCIPK family in rice, and that OsCIPK5 expression was induced in 'Yunyin' by M. grisea.
- Rice,
- CIPK,
- Gene families,
- OsCIPK5,
- M. grisea,
- qRT-PCR

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References

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Identification of CIPK Family in Rice and qRT-PCR Analysis on OsCIPK5 Induced by Magnaporthe oryzae

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