Identification and Properties of Ketoacyl-coenzyme A Synthetase Gene in <i>Primulina spinulosa</i>

LIANG Yan-ni; QIN Xin-mei; CHEN Chao-jie

doi:10.19303/j.issn.1008-0384.2019.05.006

Volume 34 Issue 5

Aug. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(5): 544-550

LIANG Yan-ni, QIN Xin-mei, CHEN Chao-jie. Identification and Properties of Ketoacyl-coenzyme A Synthetase Gene in Primulina spinulosa[J]. Fujian Journal of Agricultural Sciences, 2019, 34(5): 544-550. doi: 10.19303/j.issn.1008-0384.2019.05.006

Citation:

LIANG Yan-ni, QIN Xin-mei, CHEN Chao-jie. Identification and Properties of Ketoacyl-coenzyme A Synthetase Gene in Primulina spinulosa[J]. Fujian Journal of Agricultural Sciences, 2019, 34(5): 544-550. doi: 10.19303/j.issn.1008-0384.2019.05.006

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Identification and Properties of Ketoacyl-coenzyme A Synthetase Gene in Primulina spinulosa

doi: 10.19303/j.issn.1008-0384.2019.05.006

1.
Wuzhou University, Wuzhou, Guangxi 543000, China
2.
Guangxi Institute of Botany, Guangxi Academy of Sciences, Guilin, Guangxi 541006, China

Received Date: 2019-03-27
Rev Recd Date: 2019-04-29
Publish Date: 2019-05-28

Abstract

Abstract

Objective The ketoacyl-coenzyme A synthase (KCAS) gene relating to responses of Primulina spinulosa to drought and other stresses was studied based on the transcriptome sequencing. Method Total RNA of the gene was extracted by the improved Trizol method for sequencing with the established database. Physicochemical properties of the open reading frame (ORF), coding amino acids, and proteins of the gene were analyzed by DNAman, Finder, TMPRED, SWISS-MODEL, NetPhos 2.0, and SignaIP4.1. The phylogenetic tree was constructed accordingly. Results The full length of the KCAS gene was determined to be 1 935 bp (GenBank login number MH543314), ORF 1 599 bp, encoding 532 amino acids, molecular weight 59.82 kDa, and theoretical pI 9.16. It was a hydrophilic protein with 7 transmembrane regions, 30 possible phosphorylation sites, and one conservative domain similar to that of the chalcone synthase family. The homology between it and Dorcoceras hygrometricum (KZV788) was as high as 92%, and they were in a same phylogenetic tree. Conclusion The KCAS gene was postulated to relate to the leaf wax synthesis of P. spinosa and played an important role in drought resistance and biological response to stresses of the plant.
- Primula spinosa,
- ketoacyl-coenzyme A synthase gene,
- gene sequence analysis

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References(22)

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