Transcriptome Analysis on Anthocyanin Anabolism Genes in <i>Gynura bicolor</i> and <i>Gynura divaricata</i>

ZHANG Shao-ping; QIU Shan-lian; ZHANG Shuai; HONG Jia-min; LIN Bao-mei; ZHENG Kai-bin

doi:10.19303/j.issn.1008-0384.2019.05.003

Volume 34 Issue 5

Aug. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(5): 516-524

ZHANG Shao-ping, QIU Shan-lian, ZHANG Shuai, HONG Jia-min, LIN Bao-mei, ZHENG Kai-bin. Transcriptome Analysis on Anthocyanin Anabolism Genes in Gynura bicolor and Gynura divaricata[J]. Fujian Journal of Agricultural Sciences, 2019, 34(5): 516-524. doi: 10.19303/j.issn.1008-0384.2019.05.003

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ZHANG Shao-ping, QIU Shan-lian, ZHANG Shuai, HONG Jia-min, LIN Bao-mei, ZHENG Kai-bin. Transcriptome Analysis on Anthocyanin Anabolism Genes in Gynura bicolor and Gynura divaricata[J]. Fujian Journal of Agricultural Sciences, 2019, 34(5): 516-524. doi: 10.19303/j.issn.1008-0384.2019.05.003

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Transcriptome Analysis on Anthocyanin Anabolism Genes in Gynura bicolor and Gynura divaricata

doi: 10.19303/j.issn.1008-0384.2019.05.003

Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciencesa, Zhangzhou, Fujian 363005, China

Received Date: 2019-03-29
Rev Recd Date: 2019-05-05
Publish Date: 2019-05-28

Abstract

Abstract

Objective The core enzymes in anthocyanin anabolism were investigated, to provid a reference for further studies on the anthocyanin metabolism of G. bicolor and similar plants. Method Transcriptome of Gynura bicolor and G. divaricata were sequenced using the Illumina HiSeq 2 500 platform for the study. Result The Denovo mapping on 8 databases showed that 43 369 out of 80 798 unigenes annotated differences between the two wild vegetables. Among them, 104 unigenes were identified to relate to the anthocyanin anabolism based on two databases on the non-redundant and Swiss-Prot proteins. They included 10 chalcone synthases, 5 chalcone isomerases, 36 flavanone 3-hydroxylases, 19 dihydroflavonols, 9 anthocyanidin synthases, and 25 flavonoid 3-O-glucosyl transferases. According to the average FPKMs, 7 of the 15 differentially expressed genes (i.e., 1, 1, 3, 4, 2, and 4 genes from each of the 6 enzyme categories) between the 2 wild vegetables were found to be up-regulated, and 8 down-regulated. Conclusion There were 15 enzymes in the 104 unigenes appeared to closely associate with the anthocyanin synthesis in G. bicolor and G. divaricata.
- Gynura bicolor,
- Gynura divaricate,
- transcriptome sequencing,
- anthocyanin,
- metabolic enzyme

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References(33)

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