Cloning and Analysis of NBS-LRR Type Resistance Gene Analogues in Vigna radiata

Degenerate primers based on conserved sequences of the nucleotide binding site and 1eucine rich repeats(NBS-LRR)region from the cloned plant disease resistance genes were used to isolate resistance gene analogues(RGAs)from genomic DNA of Vigna radiata.The desired band(515bp)was cloned and sequenced.The band was named FGV-1and had been submitted to Genbank(accession number KF021265).Blastx analys showed highly homology with the reported resistance gene analogues Glycine max,Vigna unguiculata and Phaseolus vulgaris. The analysis of RGAs amino acid sequence structures suggested that FGV-1 was the core region of NBS-LRR resistance genes in Vigna radiata,which contained four conserved domains including GMGGVGKTT,LILDDVD, GSRVIVTTRD and GLPLAL.The RGAs isolated fromVigna radiata used in this study would provide the base for the further cloning of disease-resistance genes in Vigna radiata,and provide reference for the origin and evolution of Vigna radiata.