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Volume 28 Issue 7
Jul.  2013
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PAN Yi-zhe, WAN Chun-he, HUANG Yu, CHENG Long-fei, FU Guang-hua, SHI Shao-hua, CHEN Hong-mei. Prokaryotic Expression of the NS1 Gene for Avian Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2013, 28(7): 644-647. DOI: 10.19303/j.issn.1008-0384.2013.07.005
Citation: PAN Yi-zhe, WAN Chun-he, HUANG Yu, CHENG Long-fei, FU Guang-hua, SHI Shao-hua, CHEN Hong-mei. Prokaryotic Expression of the NS1 Gene for Avian Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2013, 28(7): 644-647. DOI: 10.19303/j.issn.1008-0384.2013.07.005
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Prokaryotic Expression of the NS1 Gene for Avian Tembusu Virus

  • 1. Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Science;

  • 2. College of Animal Science and Technology,Jiangxi Agricultural University

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  • Received Date: February 02, 2013
    Graphical Abstract
    • Abstract
  • A pair of primers were designed and synthesized according to the nanostructure protein (NS1) gene of avian Tenbusu virus WR strain available in GenBank.The NS1gene was amplified by RT-PCR and cloned into the prokaryotic expression vector pGEX-4T-1, then induced into E.coli with IPTG.The SDS-PAGE analysis of expressed protein demonstrated that the recombinant NS1protein of 62kD had got a high expression, which laid a good foundation for developing in molecular diagnosis and the function of the NS1gene for avian Tenbusu virus.
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    • References (20)
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