2012 Vol. 27, No. 5
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2012, 27(5): 457-460.
Abstract:
An attenuated vaccine strain of Duck Paramyxovirus(DPMVa) was selected and obtained by passage serial within SPF chicken embryo fibroblast(CEF), and some biological property of it were detemined. The DPMVa was non-pathogenic to Muscovy ducks without maternal antibody; and was non-viulence through five serial passages in one-day-old Muscovy ducks; TCID50 to CEF stable between 10-6.0~10-6.5·(0.1 mL)-1. The protection rate of the attenuated strain was above 90% when challenged with a virulent strain of DPMV 7 days after vaccination. The results indicated that attenuated vaccine strain had excellent safety, effective immunogenicity and genetic stability, which could be used as a vaccine against DPMV.
An attenuated vaccine strain of Duck Paramyxovirus(DPMVa) was selected and obtained by passage serial within SPF chicken embryo fibroblast(CEF), and some biological property of it were detemined. The DPMVa was non-pathogenic to Muscovy ducks without maternal antibody; and was non-viulence through five serial passages in one-day-old Muscovy ducks; TCID50 to CEF stable between 10-6.0~10-6.5·(0.1 mL)-1. The protection rate of the attenuated strain was above 90% when challenged with a virulent strain of DPMV 7 days after vaccination. The results indicated that attenuated vaccine strain had excellent safety, effective immunogenicity and genetic stability, which could be used as a vaccine against DPMV.
2012, 27(5): 461-464.
Abstract:
Novel Duck Reovirus(NDRV) was the pathogen of duck Hemorrhagic-necrotic hepatitis, which was an epizootic disease of ducklings, has been prevailed in China since 2005. In order to control the disease, one oil-emulsion inactivated vaccine had been developed by NDRV JM85 and the potential protective efficacy was assessed. The study indicated that this vaccine could induce strong immune responses after secondary immunization of sheldrake and the titers of neutralizing antibodies reach the peak at 28d post-immunization.84d post-immunization, the neutralizing antibodies still maintain at a high level. Baby Muscovy ducks born from mothers vaccinated with the oil-emulsion vaccine were protected successfully against NDRV infection, during the first week of life when the risk of this disease was highest. These results showed that the vaccine had a good prospect for against novel duck reovirus disease.
Novel Duck Reovirus(NDRV) was the pathogen of duck Hemorrhagic-necrotic hepatitis, which was an epizootic disease of ducklings, has been prevailed in China since 2005. In order to control the disease, one oil-emulsion inactivated vaccine had been developed by NDRV JM85 and the potential protective efficacy was assessed. The study indicated that this vaccine could induce strong immune responses after secondary immunization of sheldrake and the titers of neutralizing antibodies reach the peak at 28d post-immunization.84d post-immunization, the neutralizing antibodies still maintain at a high level. Baby Muscovy ducks born from mothers vaccinated with the oil-emulsion vaccine were protected successfully against NDRV infection, during the first week of life when the risk of this disease was highest. These results showed that the vaccine had a good prospect for against novel duck reovirus disease.
2012, 27(5): 465-469.
Abstract:
A RT-LAMP virus detection method was established for rapid,sensitive detection and early clinical diagnosis of grass carp reovirus(GCRV). Based on the VP6 gene sequences of GCRV strain HZ08 from GenBank, a set of four specific primers was designed, and the total RNA of GCRV attenuated vaccine strain was extracted as template for RT-LAMP reaction. The amplification products were confirmed by PvuⅡ restriction enzyme digestion. Then the LAMP reaction system, including Mg2+, dNTPs, betaine, and the concentration ratio of inner to outer primers was optimized. The results of the sensitivity test using recombinant plasmid contained VP6 gene as template showed that the minimum detection limit is 10 copies·L-1, a tenth the limit of RT-PCR. No cross-reactivity was observed among white spot syndrome virus(WSSV), Aeromonas hydrophila and viral nervous necrosis virus(VNNV) in RT-LAMP. Sixteen suspected grass carp of hemorrhagic disease were detected by the established assay, and consistent with the RT-PCR result, six samples got positive amplification.
A RT-LAMP virus detection method was established for rapid,sensitive detection and early clinical diagnosis of grass carp reovirus(GCRV). Based on the VP6 gene sequences of GCRV strain HZ08 from GenBank, a set of four specific primers was designed, and the total RNA of GCRV attenuated vaccine strain was extracted as template for RT-LAMP reaction. The amplification products were confirmed by PvuⅡ restriction enzyme digestion. Then the LAMP reaction system, including Mg2+, dNTPs, betaine, and the concentration ratio of inner to outer primers was optimized. The results of the sensitivity test using recombinant plasmid contained VP6 gene as template showed that the minimum detection limit is 10 copies·L-1, a tenth the limit of RT-PCR. No cross-reactivity was observed among white spot syndrome virus(WSSV), Aeromonas hydrophila and viral nervous necrosis virus(VNNV) in RT-LAMP. Sixteen suspected grass carp of hemorrhagic disease were detected by the established assay, and consistent with the RT-PCR result, six samples got positive amplification.
2012, 27(5): 470-474.
Abstract:
Resistant varieties Dular, which was known contains a resistance QTL qBlsr-11-1, and H359R, which was known contains three resistance QTL(quantitative trait loci) qBlsr3d, qBlsr5a and qBlsr5b, were used as resistance to bacterial leaf blight donor parents. On the basis of screening SSR markers closely linked to resistant QTL, we pyramided resistant QTL by bulk method combined with MAS. The results indicated that distances between the SSR markers and the target QTL estimated at 0.4-4.9cM, which will meet the basic requirements for MAS. MAS result indicated that pyramid 2-4 resistant QTL is an effective way to breed a new rice variety in Broad-spectrum resistant level.
Resistant varieties Dular, which was known contains a resistance QTL qBlsr-11-1, and H359R, which was known contains three resistance QTL(quantitative trait loci) qBlsr3d, qBlsr5a and qBlsr5b, were used as resistance to bacterial leaf blight donor parents. On the basis of screening SSR markers closely linked to resistant QTL, we pyramided resistant QTL by bulk method combined with MAS. The results indicated that distances between the SSR markers and the target QTL estimated at 0.4-4.9cM, which will meet the basic requirements for MAS. MAS result indicated that pyramid 2-4 resistant QTL is an effective way to breed a new rice variety in Broad-spectrum resistant level.
2012, 27(5): 475-480.
Abstract:
The major factors were analyzed and compared affecting the transformation frequency of indica rice mediated by Agrobacterium including medium, culture condition, subculture frequency, bacterial concentration and infection time and so on in the paper. At last a high transformation frequency of indica rice was set up. The results indicated that NMB medium for callus inducing and NB medium for callus subculturing were the best medium combination for tissue culture of indica rice. In the meanwhile, bacterial concentration, infection time and concentration ratio of KT and NAA in differential medium at the best level were modulated. The transformed calli were pre-differentiation. So the differentiation rate was improved significantly. The albino seedlings were reduced. Finally the transformation rate of indica rice was improved to 8%.
The major factors were analyzed and compared affecting the transformation frequency of indica rice mediated by Agrobacterium including medium, culture condition, subculture frequency, bacterial concentration and infection time and so on in the paper. At last a high transformation frequency of indica rice was set up. The results indicated that NMB medium for callus inducing and NB medium for callus subculturing were the best medium combination for tissue culture of indica rice. In the meanwhile, bacterial concentration, infection time and concentration ratio of KT and NAA in differential medium at the best level were modulated. The transformed calli were pre-differentiation. So the differentiation rate was improved significantly. The albino seedlings were reduced. Finally the transformation rate of indica rice was improved to 8%.
2012, 27(5): 481-484.
Abstract:
A clustered spikelets mutant(Cl-dz) controlled by single recessive gene was found in the progeny of a rice breeding material, its phenotype characteristic was that 2 or 3 spikelets cluster on the second stem in the shape of "W". Genetic analysis indicated that the trait was a recessive trait genetically controlled by single gene. The F2 mapping population was constructed by hybridization between mutant Cl-dz and G46. And the gene Cl-dz was mapped between SSR markers, RM6036 and RM1340 in chromosome 6 of rice. The genetic distance was 3.6 cM and 7.0 cM, respectively.
A clustered spikelets mutant(Cl-dz) controlled by single recessive gene was found in the progeny of a rice breeding material, its phenotype characteristic was that 2 or 3 spikelets cluster on the second stem in the shape of "W". Genetic analysis indicated that the trait was a recessive trait genetically controlled by single gene. The F2 mapping population was constructed by hybridization between mutant Cl-dz and G46. And the gene Cl-dz was mapped between SSR markers, RM6036 and RM1340 in chromosome 6 of rice. The genetic distance was 3.6 cM and 7.0 cM, respectively.
2012, 27(5): 485-490.
Abstract:
To explore high-yielding cultural techniques of ratoon rice with machine harvest and observe on growth characteristics of ratoon tillers, the combination tests of different nitrogen application time were set. The results showed that the main tillers located at the second and third node order in ratoon season with few total leaf number, no independent root, early breeding and early maturity after the main rice was harvested by hand in high cutting. While the main tillers located at the fourth and fifth node order in ratoon season with more total leaf number and independent root after the main rice was harvested by machine in low cutting. After machine harvest 7~10d, nodal root and tillers germinated at prophyll node. After machine harvest 9~12d, the first panicle branch differentiated. The heading and ripening stage delayed about 15d than that of the field which the main rice was harvested by hand in high cutting. After the main rice was harvested by machine, early and heavy application of N-fertilizer effectively promoted root, bud germination and branch, spikelet differentiation of ratoon tillers, which can increase yield significantly.
To explore high-yielding cultural techniques of ratoon rice with machine harvest and observe on growth characteristics of ratoon tillers, the combination tests of different nitrogen application time were set. The results showed that the main tillers located at the second and third node order in ratoon season with few total leaf number, no independent root, early breeding and early maturity after the main rice was harvested by hand in high cutting. While the main tillers located at the fourth and fifth node order in ratoon season with more total leaf number and independent root after the main rice was harvested by machine in low cutting. After machine harvest 7~10d, nodal root and tillers germinated at prophyll node. After machine harvest 9~12d, the first panicle branch differentiated. The heading and ripening stage delayed about 15d than that of the field which the main rice was harvested by hand in high cutting. After the main rice was harvested by machine, early and heavy application of N-fertilizer effectively promoted root, bud germination and branch, spikelet differentiation of ratoon tillers, which can increase yield significantly.
2012, 27(5): 491-497.
Abstract:
Sixty one genotypes of cauliflower germplasm were analyzed for genetic diversity based on the morphological markers in this study. The results showed that there are obvious differences among 15 morphological characters in different germplasms, the average coefficient of variation was 68.10%, and it declared that they had a great potential variation. The first five principal components of the contribution rate was 73.21%, it indicated that the first five principal components contained all morphological markers to 73.21% of the content, so the first five principal components of cauliflower morphological traits were selected, 61 varieties could be divided into four groups according to threshold of 0.77. The results showed that clustering was able to separate the materials of coming from different areas and different maturing in a certain extent.
Sixty one genotypes of cauliflower germplasm were analyzed for genetic diversity based on the morphological markers in this study. The results showed that there are obvious differences among 15 morphological characters in different germplasms, the average coefficient of variation was 68.10%, and it declared that they had a great potential variation. The first five principal components of the contribution rate was 73.21%, it indicated that the first five principal components contained all morphological markers to 73.21% of the content, so the first five principal components of cauliflower morphological traits were selected, 61 varieties could be divided into four groups according to threshold of 0.77. The results showed that clustering was able to separate the materials of coming from different areas and different maturing in a certain extent.
2012, 27(5): 498-506.
Abstract:
The aromatic components in Dangui Oolong Tea were extracted with Simultaneous Distillation Extraction(SDE) and analyzed with gas chromatography-mass spectrometry(GC-MS). Results showed that the aromatic components in Dangui Oolong Tea were different obviously from which in green tea, write tea and black tea, although they were made from the same variety of tea leaves. The aromatic components in Dangui Oolong Tea were mainly alcohols(44.27%), esters(22.15%) and hydrocarbon compounds(12.29%); others were aldehydes(8.19%), ketones(3.98%) and nitrogenous compounds(3.28%), respectively. The composition of aromatic components between green, black and white tea were different, which were alcohols(54.62%), esters(18.92%)and hydrocarbon compounds(18.11%)in green tea; alcohols(47.78%), aldehydes(26.08%) and esters(11.07%)in black tea; alcohols(49.64%), aldehydes(17.25%%)and esters(11.12%)in white tea, respectively. The variety of aromatic compounds detected from Dangui Oolong tea were nerolidol(27.1%), Geraniol(4.04%), β-linalool, trans-linaloloxide(10.94%), 3-hexenyl ester(Z)-Hexanoic(1.25%), phenylacetaldehyde(6.01%), Benzaldehyde(0.81%), beta.-Ionone(2.78%), cis-Jasmone(0.57%), Butanoic acid, 3-methyl-, Indole(1.64%), 2-phenylethyl ester(2.47%),(Z)-3-Hexen-1-ol; 3-hexenyl ester(1.56%), Tetradecane(2.02%), Farnesen(15.63%)and so on. The data showed that the aromatic composition of Dangui Oolong tea was remarkably different from other Dangui teas.
The aromatic components in Dangui Oolong Tea were extracted with Simultaneous Distillation Extraction(SDE) and analyzed with gas chromatography-mass spectrometry(GC-MS). Results showed that the aromatic components in Dangui Oolong Tea were different obviously from which in green tea, write tea and black tea, although they were made from the same variety of tea leaves. The aromatic components in Dangui Oolong Tea were mainly alcohols(44.27%), esters(22.15%) and hydrocarbon compounds(12.29%); others were aldehydes(8.19%), ketones(3.98%) and nitrogenous compounds(3.28%), respectively. The composition of aromatic components between green, black and white tea were different, which were alcohols(54.62%), esters(18.92%)and hydrocarbon compounds(18.11%)in green tea; alcohols(47.78%), aldehydes(26.08%) and esters(11.07%)in black tea; alcohols(49.64%), aldehydes(17.25%%)and esters(11.12%)in white tea, respectively. The variety of aromatic compounds detected from Dangui Oolong tea were nerolidol(27.1%), Geraniol(4.04%), β-linalool, trans-linaloloxide(10.94%), 3-hexenyl ester(Z)-Hexanoic(1.25%), phenylacetaldehyde(6.01%), Benzaldehyde(0.81%), beta.-Ionone(2.78%), cis-Jasmone(0.57%), Butanoic acid, 3-methyl-, Indole(1.64%), 2-phenylethyl ester(2.47%),(Z)-3-Hexen-1-ol; 3-hexenyl ester(1.56%), Tetradecane(2.02%), Farnesen(15.63%)and so on. The data showed that the aromatic composition of Dangui Oolong tea was remarkably different from other Dangui teas.
2012, 27(5): 507-512.
Abstract:
The correlation between organic acid components and their titratable acidity of loquat fruits were studied with path analysis. Results showed that the content of malic acid among fruit organic acids was the greatest contributor to the titratable acidity with path coefficient of PX1→Y= 0.613 9, followed by isocitrate with indirectly effecting in titratable acidity through malic acid(PX4→X1→Y＝0.446 0), and then its directly influencing in fruit acidity(PX4→Y＝0.324 8). The correlation between quinate content and fruit acidity was not obvious. The negative path coefficient from fumarate acid concentration(PX6 →Y＝-0.062 0) indicated its small but negative contribution to fruit acidity.
The correlation between organic acid components and their titratable acidity of loquat fruits were studied with path analysis. Results showed that the content of malic acid among fruit organic acids was the greatest contributor to the titratable acidity with path coefficient of PX1→Y= 0.613 9, followed by isocitrate with indirectly effecting in titratable acidity through malic acid(PX4→X1→Y＝0.446 0), and then its directly influencing in fruit acidity(PX4→Y＝0.324 8). The correlation between quinate content and fruit acidity was not obvious. The negative path coefficient from fumarate acid concentration(PX6 →Y＝-0.062 0) indicated its small but negative contribution to fruit acidity.
2012, 27(5): 518-522.
Abstract:
Using RAPD and ISSR molecular marker technology,genetic diversity of 80 samples of 8 natural populations of Bemisia tabaci collected from 7 regions of Fujian province, China was studied in this paper. The 2240 and 1776 bands were amplified respectively with 4 RAPD primers and 4 ISSR primers from the DNA of 80 samples, and among them, 2000 and 1728 bands were polymorphic respectively. The results showed that genetic diversity of B. tabaci in natural populations were quite abundant in Fujian. According to the UPGMA clustering based on the genetic distance between the populations, the 8 populations were classified into 2 groups. One group was only Longhai population(host:Codiaeum variegatum), which could be illustrated that it kept at a distance with other populations on genetic relationship. We inferred that it was possible a different biotype with others. In addition, a certain correlation was observed between the geographic distance and the genetic distance of the populations.
Using RAPD and ISSR molecular marker technology,genetic diversity of 80 samples of 8 natural populations of Bemisia tabaci collected from 7 regions of Fujian province, China was studied in this paper. The 2240 and 1776 bands were amplified respectively with 4 RAPD primers and 4 ISSR primers from the DNA of 80 samples, and among them, 2000 and 1728 bands were polymorphic respectively. The results showed that genetic diversity of B. tabaci in natural populations were quite abundant in Fujian. According to the UPGMA clustering based on the genetic distance between the populations, the 8 populations were classified into 2 groups. One group was only Longhai population(host:Codiaeum variegatum), which could be illustrated that it kept at a distance with other populations on genetic relationship. We inferred that it was possible a different biotype with others. In addition, a certain correlation was observed between the geographic distance and the genetic distance of the populations.
2012, 27(5): 524-532.
Abstract:
A total of 18 isolates with general characteristics of Rhizobium were isolated from root nodules of Chinese milk vetch from different areas of Fujian. 16S rDNA sequence analysis was conducted on these bacteria with the purpose of determine the phylogenetic analyses. The result showed that YX、Z3、GZ and ZK1 were Mesorhizobium; B3、XZ、M6 were Mesorhizobium loti or varieties, ZLH2 was Xanthomonas, M3、M10、B4、B7、ZK7 and ZQH were Agrobacterium, B6、B5、B1 and Z1 were Rhizobium. For the purpose of investigate the nodulation performance of the identified bacterial, water culture and agar tube culture were applied between the part of identified rhizobial striains and milk vetch Minzi 1、Minzi 5、Minzi 6、Minzi 7 and 80410411. This study not only further elucidate the diversity of Chinese milk vetch rhizobium, but also provides a simple and feasible method to explore the differences in the ability of different rhizobium nodulation by contrast the different of nodulation with the agar tube culture and water culture.
A total of 18 isolates with general characteristics of Rhizobium were isolated from root nodules of Chinese milk vetch from different areas of Fujian. 16S rDNA sequence analysis was conducted on these bacteria with the purpose of determine the phylogenetic analyses. The result showed that YX、Z3、GZ and ZK1 were Mesorhizobium; B3、XZ、M6 were Mesorhizobium loti or varieties, ZLH2 was Xanthomonas, M3、M10、B4、B7、ZK7 and ZQH were Agrobacterium, B6、B5、B1 and Z1 were Rhizobium. For the purpose of investigate the nodulation performance of the identified bacterial, water culture and agar tube culture were applied between the part of identified rhizobial striains and milk vetch Minzi 1、Minzi 5、Minzi 6、Minzi 7 and 80410411. This study not only further elucidate the diversity of Chinese milk vetch rhizobium, but also provides a simple and feasible method to explore the differences in the ability of different rhizobium nodulation by contrast the different of nodulation with the agar tube culture and water culture.
2012, 27(5): 533-538.
Abstract:
Under the moderate-intensity rainfall conditions, soil column leaching simulation was used to study the effects of the combined application of pig manure and shell powder or rice hull ash(RHA) calcium on characteristics of the calcium leaching, migration and transformation in the vertical aeolian sandy soil of coastal farming. The result showed that the calcium leaching was stronger, and the main forms of calcium leaching were the water-soluble calcium and adsorption calcium under 60mm of rainfall conditions. Sand soil mixed pig manure can reduce calcium fertilizer leaching, but the leaching rate was still up 79.8% to 83.1% and the leaching rate of the combined application of shell powder calcium was higher than that of RHA calcium. In addition, a moderate-intensity rainfall can make the surface shell powder calcium concentrate in the 10-15cm and migrated depth to 15 cm or 20 cm of soil, while make RHA calcium concentrate in the 15~20cm and migrated depth of 20 cm below of soil. 12.31% to 14.6% of the remaining calcium was transformed into the adsorption of calcium, and 4.7% to 5.8% of the remaining calcium exited in the water-soluble calcium form, while only a very small part of less than 0.6% was converted to non-acid-soluble calcium and non-exchange calcium.
Under the moderate-intensity rainfall conditions, soil column leaching simulation was used to study the effects of the combined application of pig manure and shell powder or rice hull ash(RHA) calcium on characteristics of the calcium leaching, migration and transformation in the vertical aeolian sandy soil of coastal farming. The result showed that the calcium leaching was stronger, and the main forms of calcium leaching were the water-soluble calcium and adsorption calcium under 60mm of rainfall conditions. Sand soil mixed pig manure can reduce calcium fertilizer leaching, but the leaching rate was still up 79.8% to 83.1% and the leaching rate of the combined application of shell powder calcium was higher than that of RHA calcium. In addition, a moderate-intensity rainfall can make the surface shell powder calcium concentrate in the 10-15cm and migrated depth to 15 cm or 20 cm of soil, while make RHA calcium concentrate in the 15~20cm and migrated depth of 20 cm below of soil. 12.31% to 14.6% of the remaining calcium was transformed into the adsorption of calcium, and 4.7% to 5.8% of the remaining calcium exited in the water-soluble calcium form, while only a very small part of less than 0.6% was converted to non-acid-soluble calcium and non-exchange calcium.
2012, 27(5): 539-543.
Abstract:
Seasonal changes and distributions of the soil microbial biomass nitrogen(SMBN) in different layers of soil in the main areas of evergreen broad-leave forest in Mt. Wuyi were studied during 2010 and 2011. The correlations among SMBN, total nitrogen(TN), organic carbon(SOC) and microbial biomass carbon(SMBC) in the soil were examined. The environmental factors affecting the results were identified. It was found that(a) SMBNs were higher in summer and autumn than in spring and winter;(b) the seasonal changes of TN was not significant;(c) SMBN of G1(0-10 cm) soil were higher than G2(10-20 cm) and G3(20-30cm);(d) SMBN was not significantly different between G2 and G3;(e) SMBN decreased from top to lower layers;and(f) SMBN, STN, SOC and SMBC in 0-30 cm soil showed a significant correlation(P<0.01). The results suggested that SMBN was significantly affected by the organic matters presented in the soil.
Seasonal changes and distributions of the soil microbial biomass nitrogen(SMBN) in different layers of soil in the main areas of evergreen broad-leave forest in Mt. Wuyi were studied during 2010 and 2011. The correlations among SMBN, total nitrogen(TN), organic carbon(SOC) and microbial biomass carbon(SMBC) in the soil were examined. The environmental factors affecting the results were identified. It was found that(a) SMBNs were higher in summer and autumn than in spring and winter;(b) the seasonal changes of TN was not significant;(c) SMBN of G1(0-10 cm) soil were higher than G2(10-20 cm) and G3(20-30cm);(d) SMBN was not significantly different between G2 and G3;(e) SMBN decreased from top to lower layers;and(f) SMBN, STN, SOC and SMBC in 0-30 cm soil showed a significant correlation(P<0.01). The results suggested that SMBN was significantly affected by the organic matters presented in the soil.
2012, 27(5): 544-549.
Abstract:
Two Acetobacter strains FJAT-13764 and FJAT-13780 were isolated from kefir grains. The observations with scanning electron microscope showed that both bacteria strains were rod-shaded with round terminals, presented in single or pairs,produced no spores. Their orerall biochemical and physiological characters indicated that these strains should be placed in the Acetobacter spp. The 16S rDNA sequence analysis indicated that FJAT-13764 and FJAT-13780 were Acetobacter orientalis and Acetobacter syzygii, respectively. This study is the first report in Acetobacter orientalis isolated from kefir grains,which provides the basic information for further researches on the micro-flora diversity in kefir grain.
Two Acetobacter strains FJAT-13764 and FJAT-13780 were isolated from kefir grains. The observations with scanning electron microscope showed that both bacteria strains were rod-shaded with round terminals, presented in single or pairs,produced no spores. Their orerall biochemical and physiological characters indicated that these strains should be placed in the Acetobacter spp. The 16S rDNA sequence analysis indicated that FJAT-13764 and FJAT-13780 were Acetobacter orientalis and Acetobacter syzygii, respectively. This study is the first report in Acetobacter orientalis isolated from kefir grains,which provides the basic information for further researches on the micro-flora diversity in kefir grain.
2012, 27(5): 550-554.
Abstract:
In order to improve the crispness leading to the taste issues in the course of boiled bamboo tablets processing and storage, crisp-keeping technology was studied in this paper. The results showed it can improve the crispness of boiled bamboo shoots by adding the amount of Sodium Tripolyphosphate and Calcium chloride. The optimum Crisp-keeping formula based on orthogonal test are following:the ratio of the composite crisp-keeping additive was sodium tripolyphosphate:calcium chloride=1:2, and Crisp-keeping concentration was 1.5 g·L-1, 45℃ of the liquid temperature, 25 min of the soaking time.
In order to improve the crispness leading to the taste issues in the course of boiled bamboo tablets processing and storage, crisp-keeping technology was studied in this paper. The results showed it can improve the crispness of boiled bamboo shoots by adding the amount of Sodium Tripolyphosphate and Calcium chloride. The optimum Crisp-keeping formula based on orthogonal test are following:the ratio of the composite crisp-keeping additive was sodium tripolyphosphate:calcium chloride=1:2, and Crisp-keeping concentration was 1.5 g·L-1, 45℃ of the liquid temperature, 25 min of the soaking time.
2012, 27(5): 555-558.
Abstract:
Perfecting the socialized service system is an important mean to promote the development of new countryside. The socialized service system will be built by taking rural sci-tech service complex as a breakthrough, through the construction of agricultural logistics complex, agricultural comprehensive experiment station and intelligent agriculture exposition park, as well as by improving the agricultural product marketing channels such as farmer-supermarket direct supplying, network marketing and a comprehensive network. The socialized service system will advance the construction of rural socialized service system and it is an effective way to solve the current rural socialized service problems and form a long-term mechanism of science and technology serving agriculture, countryside and farmers.
Perfecting the socialized service system is an important mean to promote the development of new countryside. The socialized service system will be built by taking rural sci-tech service complex as a breakthrough, through the construction of agricultural logistics complex, agricultural comprehensive experiment station and intelligent agriculture exposition park, as well as by improving the agricultural product marketing channels such as farmer-supermarket direct supplying, network marketing and a comprehensive network. The socialized service system will advance the construction of rural socialized service system and it is an effective way to solve the current rural socialized service problems and form a long-term mechanism of science and technology serving agriculture, countryside and farmers.
2012, 27(5): 559-562.
Abstract:
The protein components of seven embryogenic cultures were separated by SDS-PAGE during the early litchi somatic embryogenesis. The results showed that the protein components varied obviously during various stages of early somatic embryogenesis of litchi. Three low molecular weight proteins(6.13 kD、5.08 kD and 4.02 kD) emerged at the early developmental stages of somatic embryogenesis, which maybe played an important role in the process of the early somatic embryogenesis.
The protein components of seven embryogenic cultures were separated by SDS-PAGE during the early litchi somatic embryogenesis. The results showed that the protein components varied obviously during various stages of early somatic embryogenesis of litchi. Three low molecular weight proteins(6.13 kD、5.08 kD and 4.02 kD) emerged at the early developmental stages of somatic embryogenesis, which maybe played an important role in the process of the early somatic embryogenesis.
2012, 27(5): 513-517.
Abstract:
A new hybrid cucumber variety Mingyan 5 was bred by Sanming Institute of Agricultural Sciences with Shaxian Local Cucumber'stable inbred line as female parent and Taiwan King of Heat Tolerant Cucumber'stable inbred line as male parent. This variety s plant grew stronger and had the characteristics of moderate branch, straight and long stick-shape fruit, dark green and uniform fruit colour, black dense spines, and green sweet crispy fruit flesh. High yield, high commercial value, and cultivability in spring or autumn were its advantages. Plot yield appraisal experiments, variety comparative tests and production tests from 2007 to 2010 showed that its whole growth period in spring was about 130d. Its yield was 64500-82500 kghm-2, and increased by 2.9%-32.6% compared to Zhongnong 8. While its whole growth period in autumn was 80-85 d. Its yield was 57000-67500 kghm-2, and increased by 1.8%-21.9% compared to Zhongnong 8.
A new hybrid cucumber variety Mingyan 5 was bred by Sanming Institute of Agricultural Sciences with Shaxian Local Cucumber'stable inbred line as female parent and Taiwan King of Heat Tolerant Cucumber'stable inbred line as male parent. This variety s plant grew stronger and had the characteristics of moderate branch, straight and long stick-shape fruit, dark green and uniform fruit colour, black dense spines, and green sweet crispy fruit flesh. High yield, high commercial value, and cultivability in spring or autumn were its advantages. Plot yield appraisal experiments, variety comparative tests and production tests from 2007 to 2010 showed that its whole growth period in spring was about 130d. Its yield was 64500-82500 kghm-2, and increased by 2.9%-32.6% compared to Zhongnong 8. While its whole growth period in autumn was 80-85 d. Its yield was 57000-67500 kghm-2, and increased by 1.8%-21.9% compared to Zhongnong 8.
