1998 Vol. 13, No. 1
Display Method:
1998, 13(1): 1-8.
Abstract:
The fate and ultrastructural changes of Anabaena azollae entrapped into the developing sporocarps of Azolla pinnata were followed and characterized by laser confocal scanning image system (LCSIS) and transmission electron microscopy The entrapped vegetative Anabaena hormogonia tended to run parallelly to the long axis of the young sporocarps and underwent an active division,forming eventually an Anabaena colony,which occupied the entire indusial chamber and all the space available around the megasporangium or microsporangia Some of the vegetative cells initiated differentiation to akinetes while the opening of the chamber was closed At an intermediate stage of the sporocarp development,all the vegetative cells on the hormogonia gradually transformed into the akinete like chains Ultrastructurally the preakinetes underwent the cytoplasm reorganization and the membrane systems rearrangement The inclusions,such as carboxysomes,cyanophycin granules,ribosomes and thylakoids,varied in number,size,and their distribution in the cell along with the differentiation course of the akinete The similarities and differences in differentiation patterns and ultrastructural feature between the endophytic and free living cyanobacteria as well as their possible significance were discussed
The fate and ultrastructural changes of Anabaena azollae entrapped into the developing sporocarps of Azolla pinnata were followed and characterized by laser confocal scanning image system (LCSIS) and transmission electron microscopy The entrapped vegetative Anabaena hormogonia tended to run parallelly to the long axis of the young sporocarps and underwent an active division,forming eventually an Anabaena colony,which occupied the entire indusial chamber and all the space available around the megasporangium or microsporangia Some of the vegetative cells initiated differentiation to akinetes while the opening of the chamber was closed At an intermediate stage of the sporocarp development,all the vegetative cells on the hormogonia gradually transformed into the akinete like chains Ultrastructurally the preakinetes underwent the cytoplasm reorganization and the membrane systems rearrangement The inclusions,such as carboxysomes,cyanophycin granules,ribosomes and thylakoids,varied in number,size,and their distribution in the cell along with the differentiation course of the akinete The similarities and differences in differentiation patterns and ultrastructural feature between the endophytic and free living cyanobacteria as well as their possible significance were discussed
1998, 13(1): 9-14.
Abstract:
The maize glutamine synthetase c DNA in Escherichia coli FDB213 was firstly subcloned into pBI121 vector by using sticky end ligation Seven of seventy two colonies tested (9.7%) were identified to be inserted with MGS c DNA The orientation of inserted c DNA fragments was measured by the double digestion of the subcloned plasmid DNAs with Hind III and Bstx I endonucleases Strains P9、P15、P16 and P17 have the insert from 5’ to 3’ (sense) and P2 from 3’ to 5’ (antisense) The subcloned plasmid DNA of P16 was further introduced into Agrobacterium tumefaciens 15955 by using direct gene transformation,freeze thaw method The transformed colonies were preliminarily selected by antibiotic screening method,50 u/ml kanamycin was supplied in LB medium,then the transformation was confirmed by Southern blot analysis A11 was identified to carry MGS c DNA fragment
The maize glutamine synthetase c DNA in Escherichia coli FDB213 was firstly subcloned into pBI121 vector by using sticky end ligation Seven of seventy two colonies tested (9.7%) were identified to be inserted with MGS c DNA The orientation of inserted c DNA fragments was measured by the double digestion of the subcloned plasmid DNAs with Hind III and Bstx I endonucleases Strains P9、P15、P16 and P17 have the insert from 5’ to 3’ (sense) and P2 from 3’ to 5’ (antisense) The subcloned plasmid DNA of P16 was further introduced into Agrobacterium tumefaciens 15955 by using direct gene transformation,freeze thaw method The transformed colonies were preliminarily selected by antibiotic screening method,50 u/ml kanamycin was supplied in LB medium,then the transformation was confirmed by Southern blot analysis A11 was identified to carry MGS c DNA fragment
1998, 13(1): 15-20.
Abstract:
The keypoint to two line indica hybrid breeding is to successfully transfer PGMS genes directly or indirectly from Nongkeng 58s to indica varieties.An indica PGMS line,Mingguang 87s was newly obtained by crossing M 901s,a PGMS line derived from Nongkeng 58s which is highly stable sterility but lower outcrossing ability,with Jian 29s,a TGM line possesses high outcrossing performance.It became a completely male sterile under long day condition and restored to be fertile under short day condition.The critical temperature for fertility transformation is lower than 23 5℃.Under natural long day condition in summer,Mingguang 87s has a long (56 d ),complete (100%) and stable male sterility performance.In late autumn or winter (short day),its seed setting may reach as high as 74%.Type classification revealed that it belongs to indica subspecies.In addition,it possesses a favorable flowering performance and high combining ability.
The keypoint to two line indica hybrid breeding is to successfully transfer PGMS genes directly or indirectly from Nongkeng 58s to indica varieties.An indica PGMS line,Mingguang 87s was newly obtained by crossing M 901s,a PGMS line derived from Nongkeng 58s which is highly stable sterility but lower outcrossing ability,with Jian 29s,a TGM line possesses high outcrossing performance.It became a completely male sterile under long day condition and restored to be fertile under short day condition.The critical temperature for fertility transformation is lower than 23 5℃.Under natural long day condition in summer,Mingguang 87s has a long (56 d ),complete (100%) and stable male sterility performance.In late autumn or winter (short day),its seed setting may reach as high as 74%.Type classification revealed that it belongs to indica subspecies.In addition,it possesses a favorable flowering performance and high combining ability.
1998, 13(1): 21-26.
Abstract:
Mancang 515”,a newly bred long duration(136 d) indicajaponica cross,possesses the traits of large panicles and heavy grain weight.It shows highly resistant to blast and is nonsensitive to photo temperature.It was registered and released in 1996 in Fujian province.In yield trials in 1994-1995 in northern Fujian,it averaged 8 17% more than Jinruo 6 and 8 26% more than Shanyougui 32.The research indicated that the breeding theoty about “ideal plant morphology combining with dominancy utilization” presented by Prof.Yang Shouren has been proved in the screening indica cline rice of indica japonica hybridization.
Mancang 515”,a newly bred long duration(136 d) indicajaponica cross,possesses the traits of large panicles and heavy grain weight.It shows highly resistant to blast and is nonsensitive to photo temperature.It was registered and released in 1996 in Fujian province.In yield trials in 1994-1995 in northern Fujian,it averaged 8 17% more than Jinruo 6 and 8 26% more than Shanyougui 32.The research indicated that the breeding theoty about “ideal plant morphology combining with dominancy utilization” presented by Prof.Yang Shouren has been proved in the screening indica cline rice of indica japonica hybridization.
1998, 13(1): 27-35.
Abstract:
Diagnosis and detection are important aspect of citrus Huanglongbing research In the past,diagnosis of citrus Huanglongbing was mainly based on its sympomatology,namely,yellow shoot and mottle leaf During 1977-1978,the causative bacterium like organism (BLO) was found in sieve cells of diseased leaf under electron microscope After then,the electron microscope has been used as a major tool for diagnosis of citrus Huanglongbing disease An improvement came about when it was found that the BLO can be transmitted from citrus to periwinkle by dodder and partially purified by differential centrifugation,which made it possible to prepare antisera,both monoclonal and polyclonal antibodies,for detection and diagnosis of Huanglongbing BLO Recently,polymerase chain reaction (PCR) has been developed for detection and quanfitication of Huanglongbing BLO The PCR-based methods are highly sensitive and effective in detecting the presence of the BLO in diseased plant and insect vectors carrying the BLO Furthermore the BLO in the samples can be quantitated by quantitative PCR making it possible to compare the amounts of BLO in different samples.
Diagnosis and detection are important aspect of citrus Huanglongbing research In the past,diagnosis of citrus Huanglongbing was mainly based on its sympomatology,namely,yellow shoot and mottle leaf During 1977-1978,the causative bacterium like organism (BLO) was found in sieve cells of diseased leaf under electron microscope After then,the electron microscope has been used as a major tool for diagnosis of citrus Huanglongbing disease An improvement came about when it was found that the BLO can be transmitted from citrus to periwinkle by dodder and partially purified by differential centrifugation,which made it possible to prepare antisera,both monoclonal and polyclonal antibodies,for detection and diagnosis of Huanglongbing BLO Recently,polymerase chain reaction (PCR) has been developed for detection and quanfitication of Huanglongbing BLO The PCR-based methods are highly sensitive and effective in detecting the presence of the BLO in diseased plant and insect vectors carrying the BLO Furthermore the BLO in the samples can be quantitated by quantitative PCR making it possible to compare the amounts of BLO in different samples.
1998, 13(1): 36-40.
Abstract:
The strains with pathogenicity of Ralstonia solanacearum isolated from sweet potatoes were grown in PYG medium at 28-30℃ for 48-72 hours After sterile centrifugation (2000 r/min ),the culture fluids were filtered through 0 45 μm Millipore filters The crude toxins (metabolite) were obtained The crude toxins of Ralstonia solanacearum in sweet potatoes is sensitive to thermal treatment and it’s biological activity decreased separately 0%、28 7%、100% at 50℃、 70℃ and 100℃ waterbath for one hour The crude toxins is not sensitive to ultraviolet ray and the biological activity is unchanged under ultraviolet light for 30min、60min and 120min The crude toxins is sensitive to Pronase and lysozyme,the biological activity was destroyed and the toxicity was lost while adding papayain (4-16 u/ml) or trypsin (4-16 u/ml) to the crude toxins It’s biological activity decreased 91 6%-92 0% while adding lysozyme(15000-60000 u/ml) to the crude toxins.
The strains with pathogenicity of Ralstonia solanacearum isolated from sweet potatoes were grown in PYG medium at 28-30℃ for 48-72 hours After sterile centrifugation (2000 r/min ),the culture fluids were filtered through 0 45 μm Millipore filters The crude toxins (metabolite) were obtained The crude toxins of Ralstonia solanacearum in sweet potatoes is sensitive to thermal treatment and it’s biological activity decreased separately 0%、28 7%、100% at 50℃、 70℃ and 100℃ waterbath for one hour The crude toxins is not sensitive to ultraviolet ray and the biological activity is unchanged under ultraviolet light for 30min、60min and 120min The crude toxins is sensitive to Pronase and lysozyme,the biological activity was destroyed and the toxicity was lost while adding papayain (4-16 u/ml) or trypsin (4-16 u/ml) to the crude toxins It’s biological activity decreased 91 6%-92 0% while adding lysozyme(15000-60000 u/ml) to the crude toxins.
1998, 13(1): 41-44.
Abstract:
This paper dealt with the results in the study and practical demonstration of vertical migration of rice leaf roller in the mountain area over the past 10 years. The phenomenon of vertical migration, or flying upward along the mountain in July and flying downward in August, was further verified by five aspects. The principle of forecast in the vertical migration or at the different height with the middle and small measure, as well as the effect of the combined control were also presented.
This paper dealt with the results in the study and practical demonstration of vertical migration of rice leaf roller in the mountain area over the past 10 years. The phenomenon of vertical migration, or flying upward along the mountain in July and flying downward in August, was further verified by five aspects. The principle of forecast in the vertical migration or at the different height with the middle and small measure, as well as the effect of the combined control were also presented.
1998, 13(1): 45-48.
Abstract:
It was found that Spodoptera mauritia Baisduval is one of important pests harming Bermuda grass turf in Fujian.It occurs six to seven generations a year in Fuzhou area.The mature larva of S.mauritia Baisduval in the late sixth or early seventh generation overwinter under the sod or soil. They start occuring and harming from the second ten days or the last ten days of April.The pest harms turf more seriously in July,August and September.Ih this paper,the bionomics such as life history,development speed and life habits and control effects in Sunmit golf course turf of Fuzhou was reported.
It was found that Spodoptera mauritia Baisduval is one of important pests harming Bermuda grass turf in Fujian.It occurs six to seven generations a year in Fuzhou area.The mature larva of S.mauritia Baisduval in the late sixth or early seventh generation overwinter under the sod or soil. They start occuring and harming from the second ten days or the last ten days of April.The pest harms turf more seriously in July,August and September.Ih this paper,the bionomics such as life history,development speed and life habits and control effects in Sunmit golf course turf of Fuzhou was reported.
1998, 13(1): 49-51.
Abstract:
The paper focuses on the effect of a bacterial product 868 as the feed additive on fish meat quality The results showed that adding 868 in the culture of Tilapia mossarmbea and Anguilla Japonica increased the production without affecting the quality and fish figure Moreover, some quality of fish such as the content of fat, amino acid and water have been improved.
The paper focuses on the effect of a bacterial product 868 as the feed additive on fish meat quality The results showed that adding 868 in the culture of Tilapia mossarmbea and Anguilla Japonica increased the production without affecting the quality and fish figure Moreover, some quality of fish such as the content of fat, amino acid and water have been improved.
1998, 13(1): 52-56.
Abstract:
A limited feeding experiment was carried out with 130 kg fermented first milk for calves weaned at 45 days old.The results showed that the growth rate of treatment group in the first 6 months was slower than that of CK(350 kg milk and 90 days weaning age)and their difference was significant(P0 01).Their body weight were 99 74 kg and 134 75 kg respectively,after 6 months of age,the growth rate,first born pregnant age in month and first born milk yield between two groups were similar(P0 05).
A limited feeding experiment was carried out with 130 kg fermented first milk for calves weaned at 45 days old.The results showed that the growth rate of treatment group in the first 6 months was slower than that of CK(350 kg milk and 90 days weaning age)and their difference was significant(P0 01).Their body weight were 99 74 kg and 134 75 kg respectively,after 6 months of age,the growth rate,first born pregnant age in month and first born milk yield between two groups were similar(P0 05).
Model Design of Sustainable Agriculture on Hilly Land and the Analysis on Its Utilization Efficiency
1998, 13(1): 57-63.
Abstract:
The model of Sustainable agriculture was designed after analysing the characteristics of hilly land in the north of Fujian The research development and Utilization efficience were reported The function of eco-economic system on hilly land was estimated.
The model of Sustainable agriculture was designed after analysing the characteristics of hilly land in the north of Fujian The research development and Utilization efficience were reported The function of eco-economic system on hilly land was estimated.
