罗汉果组培苗生产体系的建立

Establishment of Tissue Culture System for Siraitia grosvenorii

摘要: 以罗汉果带节茎段为材料,对其诱导、继代、生根和移栽等条件进行研究,为罗汉果组培苗生产体系的建立提供条件。结果表明:MS+6-BA 1.0 mg·L-1+NAA 0.10 mg·L-1+蔗糖30g·L-1+琼脂6.0g·L-1为诱导培养基,诱导率可达40.0%;继代培养采用单节茎段扦插和促进腋芽生枝方法,适合的培养基为2/3改良MS+IBA 0.5mg·L-1+GA30.3mg·L-1+蔗糖40g·L-1+琼脂6.0g·L-1,增殖系数为4.0;生根培养基为1/2改良MS+IBA 0.5mg·L-1+蔗糖2.0%+活性炭0.1%+琼脂6.0g·L-1,生根率达到94%;栽培基质以泥炭土∶珍珠岩=10∶2为宜,移植成活率可达97.8%。

Abstract: The stem segment of Siraitia grosvenorii was used as the material for establishing of tissue culture system with micropropogation.The results showed that the medium of MS + 6-BA 1.0mg·L-1+ NAA 0.10mg·L-1+sucrose 30g·L-1+agar 6.0g·L-1 was suitable for inducing axillary bud with induction rate of 40.0%.Single nodes cutting and accelerating for axillary buds growth were applied in subculture and optimal medium was 2/3in modified MS + IBA 0.5 mg·L-1+ GA3 0.3 mg·L-1+ sucrose 40g·L-1+ agar 6.0 g·L-1,with proliferation coefficient 4.0.The optimal rooting medium was 1/2in modified MS +IBA 0.5mg·L-1+ sucrose2.0% + AC 0.1% + agar 6.0g·L-1,with rooting rate of 94%.The suitable transplanting medium was peat soil+ pearlite in the ratio of 10∶2,with survival rate for 97.8%.