Abstract: The zebrafish genome sequencing has been completed, and a number of specialized online databases have been established.As a model organism, zebrafish has been widely used in genetics, developmental biology and other fields.The advantages of zebrafish in fish etiology and immunology researches, such as transparent ontogenesis, mature immune system which contained both innate and adaptive immune system, and the supportive capacity for infection and replication of many fish-derived bacteria and viruses in the adults and the embryos;have made it the most popular model organism for studying infectious pathogenesis and immune response of the fish.The established zebrafish infection models and their application on vaccinology of a variety of fish pathogenic bacteria and virus have been overviewed briefly in this report.