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福建省大豆胶孢炭疽病菌对吡唑醚菌酯的抗性风险分析

阮宏椿 黄艳琴 陈巧红 陈文乐 兰成忠

阮宏椿,黄艳琴,陈巧红,等. 福建省大豆胶孢炭疽病菌对吡唑醚菌酯的抗性风险分析 [J]. 福建农业学报,2024,39(9):1−8
引用本文: 阮宏椿,黄艳琴,陈巧红,等. 福建省大豆胶孢炭疽病菌对吡唑醚菌酯的抗性风险分析 [J]. 福建农业学报,2024,39(9):1−8
RUAN H C, HUANG Y Q, CHEN Q H, et al. Risk Assessment on Resistance to Pyraclostrobin of Colletotrichum gloeosporioides [J]. Fujian Journal of Agricultural Sciences,2024,39(9):1−8
Citation: RUAN H C, HUANG Y Q, CHEN Q H, et al. Risk Assessment on Resistance to Pyraclostrobin of Colletotrichum gloeosporioides [J]. Fujian Journal of Agricultural Sciences,2024,39(9):1−8

福建省大豆胶孢炭疽病菌对吡唑醚菌酯的抗性风险分析

基金项目: 福建省科技计划公益类专项(2021R1024002);福建省农业科学院对外合作项目(DWHZ-2023-04);福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021011、XTCXGC2021017)
详细信息
    作者简介:

    阮宏椿(1980 — ),女,硕士,副研究员,主要从事植物真菌病害防治研究,E-mail:hcruan@126.com

    通讯作者:

    兰成忠(1979 — ),男,博士,副研究员,主要从事植物病害防治技术研究,E-mail:lczhong7911@126.com

  • 中图分类号: S435.29; S482.2

Risk Assessment on Resistance to Pyraclostrobin of Colletotrichum gloeosporioides

  • 摘要:   目的  探明福建省大豆胶孢炭疽菌群体对吡唑醚菌酯的敏感程度及其与其他杀菌剂的交互抗性。  方法  采用菌丝生长速率法对2023年分离自福建省5个地区的112株大豆胶孢炭疽菌进行敏感性分析,以药剂驯化法获得抗性突变体,测定抗性突变体的遗传稳定性、适合度及对4种不同杀菌剂之间的交互抗性。  结果  吡唑醚菌酯对112个菌株的EC50值介于0.06821.0309 µg·mL−1,变异系数为15.12,敏感性频率分布为连续性单峰曲线,符合偏正态分布,EC50平均值0.2036±0.1215 µg·mL−1,可作为大豆胶孢炭疽菌对吡唑醚菌酯的敏感性基线;从2株大豆胶孢炭疽菌野生菌株中诱导获得4株中抗吡唑醚菌酯的突变体,1株低抗吡唑醚菌酯的突变体,突变频率为8.3×10−4,其抗药性性状能稳定遗传,抗性突变体与其亲本菌株相比,对温度的敏感性存在差异,抗性突变体菌丝的生长速率、产孢能力及致病力,通常略低于其亲本菌株的生存适应度,抗性突变体在田间可能存在一定适合度代价,但抗性突变体具有一定的自然竞争优势;吡唑醚菌酯与不同类杀菌剂多菌灵、苯醚甲环唑、咪鲜胺之间不存在交互抗性,与同属一类的杀菌剂啶氧菌酯存在明显的交互抗性。  结论  福建省大豆胶孢炭疽菌对吡唑醚菌酯具有较高的敏感性,但存在中等抗性风险,生产中可将吡唑醚菌酯与不同类杀菌剂多菌灵、苯醚甲环唑、咪鲜胺混合或交替使用,以延缓大豆胶孢炭疽菌抗药性的产生。
  • 图  1  福建省大豆胶孢炭疽菌对吡唑醚菌酯的敏感性频率分布

    Figure  1.  Frequency distribution of EC50 of pyraclostrobin for C. gloeosporioides from Fujian

    图  2  吡唑醚菌酯与多菌灵、苯醚甲环唑、咪鲜胺和啶氧菌酯的交互抗性

    Figure  2.  Cross-resistance between pyraclostrobin and carbendazim, phenylimidazole, prochloraz, and propiconazole

    表  1  福建不同地区大豆胶孢炭疽菌对吡唑醚菌酯的敏感性

    Table  1.   Sensitivity to pyraclostrobin of C. gloeosporioides isolated from regions in Fujian

    采集地区
    Sampling region
    菌株数
    Number of isolates
    吡唑醚菌酯EC50
    EC50 of Pyraclostrobin/(µg·mL−1)
    菌株间变异系数
    Variation factor
    范围 Range 平均值 Mean
    闽东地区 Mindong region 24 0.06820.3777 0.1662±0.0633 a 5.54
    闽南地区 Minnan region 31 0.07630.3506 0.1845±0.0751 a 4.60
    闽西地区 Minxi region 27 0.08010.6428 0.2451±0.1359 a 8.02
    闽北地区 Minbei region 15 0.12841.0309 0.2387±0.2269 a 8.03
    闽中地区 Minzhong region 15 0.07250.3007 0.1933±0.0670 a 4.15
    总计 Total 112 0.06821.0309 0.2036±0.1215 a 15.12
    表中数据为平均数±标准差。同列数据后不同小写字母分别表示经Duncan氏新复极差法检验差异显著(P<0.05)。下同。
    Data are mean±SD; those with different lowercase letters on same column indicate significant difference at P<0.05 in Duncan's new multiple range test.
    下载: 导出CSV

    表  2  吡唑醚菌酯抗性突变体连续转代培养后EC50和抗性倍数的变化

    Table  2.   Changes on EC50 and resistance multiple of pyraclostrobin-resistant C. gloeosporioides mutants after continuous generational transference

    菌株
    Strain
    第一代
    1st generation
    第三代
    3rd generation
    第五代
    5th generation
    第七代
    7th generation
    EC50 /(µg·mL−1) RM EC50/(µg·mL−1) RM EC50/(µg·mL−1) RM EC50/(µg·mL−1) RM
    CG23017 0.1349 / 0.1396 / 0.1369 / 0.1344 /
    CG23017-1 1.7673 13.10 1.7823 12.77 1.7121 12.5 1.6661 12.39
    CG23017-2 0.6674 4.95 0.5275 3.78 0.4891 3.57 0.4376 3.26
    CG23017-3 1.9268 14.29 1.9084 13.67 1.8966 13.85 1.8612 13.85
    CG23052 0.1611 / 0.1647 / 0.1623 / 0.1580 /
    CG23052-1 3.0151 18.71 3.0198 18.34 2.9742 18.33 2.9574 18.72
    CG23052-2 2.7140 16.84 2.7191 16.51 2.7200 16.76 2.6425 16.73
    RM为抗性倍数。
    RM: Resistance multiple.
    下载: 导出CSV

    表  3  抗性突变体及其亲本菌株对温度的敏感性测定

    Table  3.   Mycelial growth of resistant mutants and parents under different temperatures

    菌株 Strain 菌落直径 Colony diameter/mm
    16 ℃ 20 ℃ 25 ℃ 28 ℃ 35 ℃
    CG23017 31.33±0.58 cd 43.33±1.53 c 59.33±2.31 c 63.67±1.53 b 20.67±1.53 a
    CG23017-1 28.67±0.58 e 41.33±1.15 cd 58.33±1.15 c 60.67±1.53 b 18.67±3.06 ab
    CG23017-2 25.33±1.15 f 37.67±1.15 e 56.67±1.53 c 60.33±2.31 b 19.33±1.53 ab
    CG23017-3 29.67±1.53 de 39.33±1.53 de 57.33±1.53 c 60.67±2.31 b 19.67±1.15 ab
    CG23052 35.67±2.08 a 48.33±1.15 a 72.67±0.58 a 74.67±1.53 a 17.67±1.53 ab
    CG23052-1 34.33±1.53 ab 45.67±1.15 b 69.67±1.15 b 71.67±1.53 a 18.33±0.58 ab
    CG23052-2 32.33±1.53 bc 46.33±0.58 ab 68.33±1.53 b 71.33±2.52 a 16.67±2.52 b
    下载: 导出CSV

    表  4  抗性突变体及其亲本菌株菌丝生长速率、产孢能力、致病力测定

    Table  4.   Mycelial growth rate, sporulation per area, and disease index of resistant mutants and parents

    菌株
    Strain
    菌丝生长速率
    Mycelium growth rate/(mm·d−1)
    单位面积产孢量
    Sporulation per area/(×103个·mm−2)
    病情指数
    Disease index
    CG23017 9.1±0.22 b 4.58±0.27 ab 47.52±2.34 a
    CG23017-1 8.67±0.22 b 4.55±0.19 ab 40.37±4.56 a
    CG23017-2 8.62±0.33 b 4.32±0.08 b 27.08±4.83 b
    CG23017-3 8.67±0.33 b 4.50±0.26 ab 42.48±10.06 a
    CG23052 10.67±0.22 a 4.77±0.25 a 41.48±8.82 a
    CG23052-1 10.24±0.22 a 4.74±0.23 a 26.63±6.32 b
    CG23052-2 10.19±0.36 a 4.61±0.2 ab 37.43±0.89 ab
    下载: 导出CSV
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  • 收稿日期:  2024-05-23
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