Effect of arbuscular mycorrhizal fungi on the growth and secondary metabolism of Taxus chinensis (seedling stage )
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摘要:
目的 研究丛枝菌根真菌（arbuscular mycorrhizal fungi，AMF）对南方红豆杉苗期生长及次生代谢的影响，揭示南方红豆杉与AMF的共生关系，为南方红豆杉的种植和利用提供科学依据。 方法 以南方红豆杉幼苗（Taxus wallichiana var. Mairei）为材料，盆栽试验条件下，在其根部接种AMF根内根孢囊霉菌（Rhizophagus intraradices）和摩西斗管囊霉菌（Funneliformis mosseae）以及两菌种的混合菌剂，本试验接种与种植同步进行。研究AMF对南方红豆杉苗期的苗高、主根长、地径等植物生长指标、土壤理化性质以及次生代谢物紫杉醇含量的影响。 结果 （1）接种AMF能显著促进南方红豆杉苗木株高、地径、根长和一级枝数的增长，其中接种R. intraradices和F. mosseae对株高和根长的增长达到显著水平，接种R. intraradices对地径的增长效果最显著，接种F. mosseae对一级枝数的增加效果最好；（2）AMF的生长指标与土壤速效磷含量、土壤碱解氮和土壤速效钾含量等土壤理化性质呈显著相关性，其中侵染率与速效磷呈现显著负相关性（p＜0.05），而其他指标例如碱解氮、速效钾等于生长指标均呈现显著相关性（p＜0.05）；（3）接种AMF均能显著提高紫杉醇的含量，其中接种R. intraradices 效果最好。 结论 通过比较不同AMF对南方红豆杉苗期生长和次生代谢的影响，发现根内根孢囊霉菌与南方红豆杉的共生模式更能促进其生长和次生代谢产物的积累。 Abstract:Objective To investigate the impacts of AM fungi on the growth and secondary metabolism of Taxus chinensis seedlings, to discover the symbiotic relationship between Taxus chinensis and AM fungi, and to offer a scientific foundation for Taxus chinensis planting and use. Method Taxus chinensis seedlings were used as materials, and the roots were inoculated with the arbuscular mycorrhizal fungi (AMF) Rhizophagus intraradices and Funneliformis mosseae during the potting test, as well as a mixture of the two strains, and the inoculation was timed to coincide with planting in this experiment. The effects of AMF on plant growth markers as seedling height, primary root length, ground diameter, soil physicochemical qualities, and secondary metabolism paclitaxel content were investigated in Taxus chinensis seedlings. Result The results showed that: (1) AMF inoculation could significantly promote the growth of plant height, ground diameter, root length, and number of primary branches of Taxus chinensis seedlings, among which the inoculation of R. intraradices and F. mosseae reached a significant level of growth of plant height and root length, and the inoculation of R. intraradices had the most significant effect on the growth of ground diameter, and the inoculation of F. mosseae had the best influence on the increase of the number of first-order branches. (2) The growth indexes of AMF showed significant correlation with soil physicochemical properties such as soil quick-acting phosphorus content, soil alkaline dissolved nitrogen , and soil quick-acting potassium content. The infestation rate showed a significant negative correlation with the quick-acting phosphorus (p＜0.05), and other indexes such as alkaline dissolved nitrogen , quick-acting potassium , and growth indexes all showed a significant correlation.(3) Inoculation with AMF might greatly increase the content of paclitaxel, with R. intraradices having the strongest effect. Conclusion By analyzing the impacts of several AM fungi on Taxus chinensis growth and secondary metabolism during the seedling stage, it was discovered that the symbiotic pattern of Rhizophagus intraradices and Taxus chinensis could better promote growth and accumulation of secondary metabolism products. -
Key words:
- Arbuscular mycorrhizal fungi /
- Taxuschinensis /
- secondarymetabolism /
- paclitaxel content /
- interactions
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图 2 不同处理组对南方红豆杉幼苗生长指标的影响
小图中具有不同字母的处理差异显著（P＜0.05）。下同
Figure 2. Effect of AM fungi on seedling growth index of Taxus chinensis
In each panel,the treaments with different letters are significantly different (P ＜ 0.05).The same below
图 3 不同处理对南方红豆杉幼苗紫杉醇浓度的影响
Figure 3. Effects of different treatments on paclitaxel concentration in southern red bean curd seedlings
表 1 不同 AM 真菌的土壤理化性质（平均值+标准误）
Table 1. Soil pHysicochemical properties of different AM fungi Chinensis（mean+SE）
处理
Treatment酸碱度
PH含水量
Moisture
content/
(×100 %)速效钾
Rapidly available
potassium/
(μg·mL−1)碱解氮
Alkali hydrolyzale
nitrogen/
(mg·kg−1)速效磷
Rapidly available
phosphorus/
(mg·L−1)侵染率
Colonization
rate/
(×100%)CK 6.57±0.52b 0.41±0.036a 1.46±0.56c 1.58±0.76b 6.13±0.12a 0.02±0.001c GM 6.01±0.29d 0.41±0.053a 1.76±0.25b 2.85±1.16a 4.67±0.05b 0.18±0.058b GI 6.31±0.92c 0.42±0.019a 2.14±0.38a 3.11±3.16a 4.48±0.50b 0.28±0.03a MA 6.99±0.32a 0.41±0.034a 1.81±1.2b 1.93±1.91b 6.88±0.13a 0.17±0.09b 同一列数据中字母不同者表示差异显著（P ＜ 0.05)。
Date with different letters are significantly different (P ＜ 0.05) in the same column.
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表 2 土壤理化性质与生长指标相关性分析
Table 2. Correlation analysis between soil pHysicochemical properties and growth indicators
项目
Item速效钾
Rapidly
available
potassium碱解氮
Alkali
hydrolyzale
nitrogen速效磷
Rapidly
Available
phosphorus酸碱度
Pondus
hydrogenii侵染率
Colonization
rate根长
Root
length地径
Grounddiameter株高
Plant
height速效钾 Rapidly available potassium 1 碱解氮 Alkali hydrolyzale nitrogen 0.618* 1 速效磷 Rapidly Available phosphorus −0.493 −0.818** 1 酸碱度 Pondus hydrogenii −0.230 −0.559 0.814** 1 侵染率 Colonization rate 0.855** 0.789** −0.618* −0.336 1 根长 Root length 0.683* 0.812** −0.868** −0.795** 0.747** 1 地径 Ground diameter 0.709** 0.868** −0.883** −0.781** 0.821** 0.917** 1 株高 Plant height 0.534 0.782** −0.837** −0.795** 0.621* 0.845** 0.878** 1 注：*表示显著相关（P ＜ 0.05），**表示极显著相关P ＜ 0.01。
Note:* Indicate significant correlation（P ＜ 0.05），**Indicating extremely significant correlation（P ＜ 0.01）.
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