枯草芽孢杆菌B2-GFP菌株对甜椒幼苗生长和生理特性的影响

赵莉; 尚烨; 闫庚洋; 刘菊; 张菊平

枯草芽孢杆菌B2-GFP菌株对甜椒幼苗生长和生理特性的影响

河南科技大学园艺与植物保护学院，河南　洛阳，471000

基金项目: 河南省自然科学基金项目（242300421318）中国烟草总公司重大科技项目[110202101051（LS-11）]；
中国烟草总公司河南省公司重点科技项目（2023410000240022）

详细信息

作者简介:
赵莉（1996 —），女，硕士研究生，主要从事蔬菜栽培生理研究，E-mail：653597172@qq.com

通讯作者:
张菊平（1968 —），女，博士，教授，主要从事蔬菜栽培与遗传育种研究，E-mail：jupingzhang@163.com

中图分类号: S641.3
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出版历程
- 收稿日期: 2024-01-15
- 录用日期: 2024-04-12
- 修回日期: 2024-03-19
- 网络出版日期: 2024-06-26

Application of Bacillus subtilis B2-GFP to Promote Growth of Sweet Pepper Seedlings

College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, Henan　471000, China

摘要

摘要: 目的筛选能促进甜椒幼苗生长的枯草芽孢杆菌B2-GFP菌株发酵液适宜浓度，为研发菌肥作用于蔬菜提供依据。方法以甜椒硕源808为材料，设置枯草芽孢杆菌B2-GFP菌株发酵液T1（1×10⁵ CFU·mL⁻¹）、T2（1×10⁶ CFU·mL⁻¹）、T3（1×10⁷ CFU·mL⁻¹）、T4（1×10⁸ CFU·mL⁻¹）4个浓度梯度处理，每隔7 d向植株浇灌菌株发酵液，连续浇灌3次，每次每株浇灌5 mL，21 d时测定甜椒幼苗生长指标、生物量积累、叶片光合特性、叶绿素荧光参数、根系抗氧化酶活性、根系形态建成及根系活力指标。结果与对照相比，不同浓度枯草芽孢杆菌B2-GFP菌株发酵液可提高甜椒株高、叶面积、叶绿素含量、植株生物量，以1×10⁶ CFU·mL⁻¹的增幅最大。同时，该浓度显著提高了叶片蒸腾速率（T_r）、气孔导度（G_s）、最大荧光（F_m）、PSⅡ实际光化学效率（Ф_PSⅡ）和光化学荧光猝灭系数（qP），降低了胞间CO₂浓度（C_i）和叶绿素基础荧光（F_o）；提高甜椒根系过氧化物酶（POD）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）活性及根系活力。结论在甜椒移栽后浇灌1×10⁶ CFU·mL⁻¹B2-GFP菌株发酵液可促进甜椒植株生长和根系形态建成，增强叶片光合能力，提高生物量积累、根系抗氧化酶活性和根系活力。
- 枯草芽孢杆菌 /
- 甜椒 /
- 生长 /
- 生理特性 /
- 光合特性
Abstract: Objective Effects of applying Bacillus subtilis B2-GFP culture broth on the growth and physiology of sweet pepper seedlings were studied. Methods In a pot experiment, seedlings of sweet pepper Shuoyuan 808 were treated with B. subtilis B2-GFP culture broths at the concentrations of 1×10⁵CFU·mL⁻¹ (T1), 1×10⁶CFU·mL⁻¹ (T2), 1×10⁷CFU·mL⁻¹ (T3), and 1×10⁸CFU·mL⁻¹ (T4), along with non-treatment control (CK). The transplanted sweet pepper seedlings were given 5 mL 1×10⁶CFU·mL_⁻¹B2-GFP culture broth every 7 d for 3 times. Growth index, biomass accumulation, leaf photosynthesis, and chlorophyll fluorescence parameters as well as antioxidant enzyme activities, morphological formation, and root vitality of the plants were monitored at 21 d. Results Application of the B2-GFP culture broth increased the plant height and biomass as well as the leaf area and chlorophyll content over CK. The greatest effect was observed under T2, which also accentuated the leaf transpiration rate (T_r), stomatal conductance (G_s), maximum fluorescence (F_m), photosystem II potential Activity (Ф_PSⅡ), and photochemical fluorescence quenching coefficient (qP) as well as the activities of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), phenylalanine ammonia lyase (PAL), and polyphenol oxidase (PPO) in the roots but reduced the intercellular CO₂ concentration (C_i) and chlorophyll basic fluorescence (F_o). Conclusion When the transplanted sweet pepper seedlings were given 1×10⁶CFU·mL⁻¹ B2-GFP culture broth, all monitored indicators on the plant growth and root development including leaf photosynthetic parameters and biomass and root antioxidant enzymes activities and vitality were significantly improved in 3 weeks.
- Bacillus subtilis /
- sweet pepper /
- growth /
- physiology /
- photosynthesis

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图 1 枯草芽孢杆菌B2-GFP菌株发酵液对甜椒生物量的影响

不同小写字母表示在0.05水平上差异显著。下图同。

Figure 1. Effect of B2-GFP culture broth on biomass of sweet pepper seedlings

Data with different lowercase letters indicate significant differences at P＜0.05. Same for below.

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图 2 枯草芽孢杆菌B2-GFP菌株发酵液对甜椒叶片光合特性的影响

Figure 2. Effect of B2-GFP culture broth on leaf photosynthetic characteristics of sweet pepper seedlings

下载: 全尺寸图片幻灯片

图 3 枯草芽孢杆菌B2-GFP菌株发酵液对甜椒根系抗氧化酶活性的影响

Figure 3. Effect of B2-GFP culture broth on root antioxidase activity of sweet pepper seedlings

下载: 全尺寸图片幻灯片

图 4 枯草芽孢杆菌B2-GFP菌株发酵液处理甜椒的根系扫描图

Figure 4. Scanning image of sweet pepper roots treated with B2-GFP culture broth

下载: 全尺寸图片幻灯片

图 5 枯草芽孢杆菌B2-GFP菌株发酵液对甜椒根系活力的影响

Figure 5. Effect of B2-GFP culture broth on activity of sweet pepper seedling roots

下载: 全尺寸图片幻灯片

表 1 枯草芽孢杆菌B2-GFP菌株发酵液对甜椒生长指标的影响

Table 1. Effect of B2-GFP culture broth application on growth of sweet pepper seedlings

处理 Treatment	株高 Plant height/cm	茎粗 Stem diameter/mm	叶面积 Leaf area/cm²	叶绿素相对含量 SPAD
CK	11.90±0.10 d	4.05±0.20 ab	15.79±0.55 c	45.69±0.26 b
T1	13.60±0.24 b	3.94±0.10 bc	18.07±1.09 b	47.42±0.79 ab
T2	14.58±0.27 a	4.35±0.20 a	20.62±1.16 a	48.12±1.09 a
T3	12.98±0.26 c	3.53±0.20 d	18.06±1.19 b	47.67±0.82 ab
T4	12.10±0.17 d	3.60±0.25 cd	16.69±0.88 bc	47.58±1.54 ab
同列不同小写字母表示在0.05水平的差异显著性。下表同。 Data with different lowercase letters on same column indicate significant differences at P＜0.05. Same for below.

下载: 导出CSV

表 2 枯草芽孢杆菌B2-GFP菌株发酵液对甜椒叶片叶绿素荧光参数的影响

Table 2. Effect of B2-GFP culture broth on leaf chlorophyll fluorescence parameters of sweet pepper seedlings

处理 Treatment	叶绿素基础荧光F_o	最大荧光 F_m	PSⅡ最大光能转换效率F_v/F_m	PSⅡ实际光化学效率Φ_PSⅡ	光化学荧光猝灭系数qP	非光化学猝灭效率NPQ
CK	3995±140 a	19308±1632 b	0.825±0.004 abc	0.520±0.008 bc	0.67±0.006 b	1.54±0.13 a
T1	3811±95 ab	20334±1103 ab	0.827±0.006 ab	0.533±0.012 ab	0.69±0.006 a	1.57±0.25 a
T2	3576±197 b	22714±1638 a	0.831±0.001 a	0.539±0.006 a	0.70±0.012 a	1.81±0.16 a
T3	3818±163 ab	22610±496 a	0.820±0.002 c	0.525±0.004 abc	0.65±0.015 c	1.94±0.08 a
T4	3822±146 ab	21450±2207 ab	0.822±0.001 bc	0.511±0.014 c	0.64±0.010 c	1.69±0.40 a

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表 3 枯草芽孢杆菌B2-GFP菌株发酵液对甜椒根系生长特性的影响

Table 3. Effect of B2-GFP culture broth on growth of sweet pepper seedling roots

处理 Treatment	总根长 Length/cm	根总表面积 Surf area/cm²	根系总体积 Root volume/cm³	根尖数 Tips	分叉数 Forks
CK	435.24±41.12 b	55.16±5.93 b	0.642±0.092 bc	1737±175 b	2024±651 ab
T1	498.32±57.70 ab	74.20±11.64 a	0.757±0.075 ab	1401±182 b	1802±405 ab
T2	564.47±48.07 a	70.96±9.45 ab	0.881±0.110 a	2179±273 a	2607±768 a
T3	449.93±85.85 b	53.31±7.19 b	0.631±0.134 bc	1553±112 b	2039±793 ab
T4	388.92±35.59 b	53.39±9.88 b	0.543±0.097 c	1543±218 b	1405±426 b

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