桑葚不同果实发育阶段的代谢组学分析

钟秋珍; 林旗华; 张泽煌

桑葚不同果实发育阶段的代谢组学分析

福建省农业科学院果树研究所，福建福州 350013

基金项目: 福建省属公益类科研院所基本科研专项（2020R1028002）

详细信息

作者简介:
钟秋珍（1973 —），女，高级农艺师，主要从事果树良种选育与栽培研究，E-mail：3249015@qq.com

通讯作者:
张泽煌（1973 —），男，研究员，主要从事果树良种选育与栽培研究，E-mail：419091816@qq.com

中图分类号: S663.9
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出版历程
- 收稿日期: 2027-07-16
- 修回日期: 2024-09-05
- 网络出版日期: 2024-11-11

Metabolomics analysis of mulberry fruits at different developmental stages

Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou,　350013, China

摘要

摘要: 目的研究桑葚果实发育过程中的代谢组分变化规律。方法以桑葚品种粤椹大十绿果期、转色期和成熟期的果实为样本材料，进行广泛靶向代谢组学分析，并基于差异富集代谢物（Differentially accumulated metabolites, DAMs）进行KEGG代谢途径富集分析。结果从粤椹大十3个时期果实样本中检测到1146种代谢组分，以P＜0.05且VIP＞1.0为标准，其中483种代谢物被鉴定为DAMs，包括51种积累水平在整个果实发育过程中均显著差异的DAMs。2种类黄酮和1种花色苷在桑葚发育过程中显著提高。基于DAMs进行KEGG分析表明，α-亚麻酸代谢和亚油酸代谢在绿果期发育到转色期过程中显著富集，抗坏血酸和藻酸盐代谢在转色期发育到成熟期过程中显著富集，而亚油酸代谢和角质/木栓和蜡生物合成在整个果实发育过程中显著富集。进一步分析发现，尽管粤椹大十桑葚果实发育过程中包含6种上调积累和1种下调积累的亚油酸代谢组分，但从整体上看亚油酸积累水平呈下降趋势。结论发现51种DAMs可能持续参与了桑葚果实整个发育过程，其中花色苷与类黄酮显著提高，亚油酸代谢途径中的7种关键代谢组分可能影响其品质风味形成。研究结果有助于更好地了解桑葚成熟过程中营养成分的动态变化规律，为揭示桑葚果实品质风味形成机制和筛选优质桑葚种质奠定了基础，同时也为桑葚采摘期的判定提供了科学依据。
- 桑葚 /
- 果实发育 /
- 代谢组学 /
- KEGG代谢途径 /
- 亚油酸
Abstract: Objectives To study the changes in metabolic components at different development stages of mulberry fruits. Methods a widely targeted metabolomics analysis was conducted on samples from the green fruit stage, color transformation stage, and mature stage of Morus alba cv. yueshen dashi. And the KEGG metabolic pathway enrichment was then analysed based on differentially accumulated metabolites (DAMs). Results A total of 1146 metabolic components were identified from three developmental stages of mulberry fruit. Using P＜0.05 and VIP＞1.0 as the criteria, 483 metabolites were screened as DAMs, including 51 DAMs with significant differences in accumulation levels throughout the whole comparison groups. Two flavonoids and one anthocyanin were significantly increased during the development of mulberries. DAMs based KEGG metabolic pathway analysis found that α-linolenic acid metabolism and linoleic acid metabolism were significantly enriched during the green fruit development to color transition period, while ascorbate and aldarate metabolism were significantly enriched during the color transition period to maturity period, while linoleic acid metabolism and cutin, suberine and wax biosynthesis were significantly enriched throughout the fruit development process. Further analysis revealed that although there were six upaccumulated and one downaccumulated linoleic acid components in the development process of mulberry fruit, the overall accumulation level of linoleic acid showed a downward trend. Conclusions This study found that 51 DAMs might involve in the entire development process of mulberry fruits, the anthocyanins and flavonoids were significant increased, and 7 key metabolic components in the linoleic acid metabolism pathway may affect the formation of its quality and flavor. These results contributed to a better understanding of the dynamic changes in nutritional components during the ripening process of mulberries, and laid the foundation for revealing the mechanism of mulberry fruit quality and flavor formation and screening high-quality mulberry germplasm. Additionally, this research provided scientific basis for determining the picking period of mulberries.
- Mulberry /
- Fruit development /
- Metabolomics /
- KEGG metabolic pathway /
- Linoleic acid

HTML全文

图 1 不同发育时期的桑葚果实表型

DS-Q、DS-Z和DS-C分别表示绿果期、转色期和成熟期。下同。

Figure 1. Phenotypes of mulberry fruits at different developmental stages

DS-Q, DS-Z and DS-C represent the green fruit stage, color transition stage and maturity stage, respectively.The same as below.

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图 2 桑葚果实样本色谱图

A和B分别表示正、负离子模式下色谱图。

Figure 2. Chromatogram of mulberry fruit samples

A and B represent chromatograms in positive and negative ion modes, respectively.

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图 3 基于代谢组学的不同发育阶段桑葚果实样本聚类

Figure 3. Cluster analysis of mulberry fruit samples at different developmental stages based on metabolomics

下载: 全尺寸图片幻灯片

图 4 桑葚果实不同发育时期的差异富集代谢物鉴定

A和B分别为差异富集代谢物的数目变化统计和韦恩图

Figure 4. Identification of DAMs at different development stages of mulberry fruit

A and B represent the statistics and Venn diagram of the DAM changes respectively.

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图 5 基于桑葚果实不同发育时期DAMs的KEGG代谢途径富集分析

A、B和C分别表示DS-Z vs DS-Q、DS-C vs DS-Z和DS-C vs DS-Q比较组。

Figure 5. Analysis of KEGG metabolic pathway enrichment based on DAMs of different development stages of mulberry fruit

A, B and C represent the comparison groups of DS-Z vs DS-Q, DS-C vs DS-Z and DS-C vs DS-Q.

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图 6 桑葚果实发育过程中亚油酸代谢途径变化

红色表示该代谢物在对应样本中上调积累，绿色表示下调积累。从左往右对应的3种样本分别为绿果期、转色期和成熟期的桑葚果实。

Figure 6. Changes in linoleic acid metabolism pathways during the development of mulberry fruits

Red color indicates upaccumulation of the metabolite in the corresponding sample, while green color indicates downaccumulation. The three corresponding samples from left to right are samples of green fruit stage, color transition stage, and mature stage of mulberry fruits.

下载: 全尺寸图片幻灯片

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