6类茶叶中15种农药残留过程中产生的基质效应研究

陈璐; 李怀平; 王顾希; 唐祥凯; 冯德建; 魏雨婷

6类茶叶中15种农药残留过程中产生的基质效应研究

Study on matrix effects in the detection of 15 pesticide residues in 6 types of tea leaves

摘要

摘要:
目的对气相色谱-串联质谱法测定6类茶叶中15种农药残留过程中产生的基质效应进行分析，并就日常检测中常见批量样品同时处理时可采用的替代基质进行探讨。
方法茶叶样品经QuEChERS前处理，配制质量浓度在0.01～0.32 mg·L⁻¹的溶剂标准曲线和基质标准曲线，经GC-MS/MS测定后计算基质效应。
结果 6类茶叶中15种农药检测过程中均产生增强基质效应，其中以增强强基质效应为主，基质效应区间范围1.35%～411.58%，强、中、弱基质效应分别为73.33%、23.33%、3.33%。以红茶为替代基质测定15种农药时，主要以产生增强弱基质效应为主，整体区间范围为|0.62%|～|59.22%|，强中弱基质效应分别为1.33%、14.67%、84.00%；以混合茶叶为代表基质测定15种农药时，主要以产生抑制弱基质效应为主，整体区间范围为|0.09%|～|48.09%|，强中弱基质效应分别为0、13.33%、86.67%。
结论茶叶样品基质复杂，在农残检测时使用基质标曲分析可降低基质效应，提高检测结果的准确度。使用混合茶叶样品作为替代基质配制基质标曲对茶叶农残进行定量分析时，可有效提高批量样品的分析效率。

Abstract:
Objective Matrix effects in detecting 15 pesticide residues in six types of tea using gas chromatography-tandem mass spectrometry (GC-MS/MS) with a sample pretreatment of QuEChERS were analyzed for assay improvement.
Methods Tea samples were pretreated with QuEChERS, and solvent and matrix standard curves for the mass concentrations of 0.01-0.32 mg·L⁻¹ prepared. After a GC-MS/MS determination, the matrix effects were calculated.
Results Matrix effects were enhanced in the determination that ranged from 1.35-411.58% with the strong, medium, and weak matrix effects of 73.33%, 23.33%, and 3.33%, respectively. When black tea was used as an alternative matrix, they ranged 0.62-59.22% with the strong, medium, and weak matrix effects at 1.33%, 14.67% and 84.00%, respectively. Whereas, when a mixed tea was applied as an alternative matrix, suppressed matrix effects resulted at 0.09-48.09% with the strong, medium, and weak matrix effects at 0, 13.33% and 86.67%.
Conclusion The matrix effects in the pesticide residue detection on teas were complex. Application of a matrix standard curve reduced the matrix effects and improved the determination accuracy. By using mixed tea sample as an alternative matrix, the analysis efficiency was significantly enhanced.

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