食用玫瑰烈火组培快繁关键技术研究

陈剑勇

doi:10.19303/j.issn.1008-0384.2022.009.009

食用玫瑰烈火组培快繁关键技术研究

doi: 10.19303/j.issn.1008-0384.2022.009.009

陈剑勇

福建林业职业技术学院，福建　南平　353000

基金项目: 福建省南平市自然科学基金项目（N2021J010）

详细信息

作者简介:
陈剑勇（1980−），男，硕士，高级实验师，研究方向：植物生物技术（E-mail：915103491@qq.com）

中图分类号: S 685.12
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- 被引次数: 0
出版历程
- 收稿日期: 2022-05-28
- 修回日期: 2022-08-30
- 网络出版日期: 2022-10-21
- 刊出日期: 2022-09-30

Key Techniques for Tissue Culture and Rapid Propagation of Edible Rose Flaming Fire

CHEN Jianyong

Fujian Forestry Vocational & Technical College, Nanping, Fujian 353000, China

摘要

摘要: 目的研究食用玫瑰烈火的组培快繁技术，建立组培快繁技术体系，解决烈火食用玫瑰种苗工厂化生产的瓶颈问题。方法通过对比试验，筛选外植体最佳的消毒药剂及处理时间组合；通过正交试验，筛选芽苗增殖培养最佳的植物生长调节剂种类及浓度水平，筛选最佳的生根基本培养基、植物生长调节剂种类及浓度水平。结果（1）食用玫瑰烈火的外植体消毒以0.1%HgCl₂ 处理8 min，外加6%次氯酸钠溶液处理3~6 min效果最佳；（2）芽苗增殖最优培养基配方为MS+6-BA 2.0 mg·L⁻¹ +NAA 0.2 mg·L⁻¹+ TDZ 0. 06 mg·L⁻¹，增殖系数达3.47；（3）芽苗生根最佳培养基为改良1/2MS+ NAA0.2 mg·L⁻¹+IBA0.01 mg·L⁻¹，生根率达89.44%，根系健壮。结论通过外植体消毒对比试验，芽增殖、生根培养正交试验所建立起的食用玫瑰烈火组培快繁技术体系，可为烈火食用玫瑰种苗工厂化生产提供技术支撑，促进食用玫瑰在农业上的推广应用。
- 食用玫瑰 /
- 组织培养 /
- 芽诱导 /
- 增殖 /
- 生根
Abstract: Objective To systematically study the tissue culture and rapid propagation technology of edible rose flaming fire, and establish the tissue culture and rapid propagation technology system to solve the bottleneck problem of seedling industrialized production of edible rose flaming fire. Methods The best disinfectant and treatment time of explants were selected through comparative test; Through orthogonal test, the best plant growth regulators and concentration levels were selected for bud proliferation culture, and the best rooting basic medium, and plant growth regulators and their concentrations were selected. Result (1) The explants treated with 0.1% HgCl₂ for 8 min and then 6% sodium hypochlorite solution for 3–6 min was the best for tissue cluture of flaming fire; (2) The optimum medium formula for bud propagation was MS+6-BA 2.0 mg·L^-1+NAA 0.2 mg·L^-1+TDZ 0.06 mg·L^-1, and the multiplication coefficient was 3.47; (3) The improved rooting medium for bud seedlings was 1/2MS+NAA 0.2 mg·L^-1+IBA 0.01 mg·L^-1, the rooting rate of which was 89.44%, and the root system was strong. Conclusion The tissue culture and rapid propagation technology system of edible rose flaming fire established by the comparative experiment for explant disinfection, and the orthogonal experiment for bud proliferationand rooting culture, can provide technical support for the industrialized production of edible rose seedlings of flaming fire, and promote the popularization and application of edible roses in agriculture.
- Edible roses /
- tissue culture /
- bud induction /
- proliferation /
- rooting

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图 1 芽苗在B₆（A）和B₅（B）培养基上的增殖生长情况

Figure 1. Growth of buds and seedlings on B₆ (A) and B₅ (B) medium

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图 2 芽苗在C₉（A）和C₈（B）培养基上的生根情况

Figure 2. Growth of roots on C₉ (A) and C₈ (B) medium

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表 1 各处理试验方案

Table 1. Codes of various test designs、treatments and levels

外植体消毒时间 Explant disinfection			芽苗增殖培养基配方 Shoot proliferation test				生根培养基配方 Rooting formula test
代号	0.1%HgCl₂/min	6%次氯酸钠溶液/min	代号	6-BA/(mg·L⁻¹）	NAA/（mg·L⁻¹）	TDZ/（mg·L⁻¹）	代号	基本培养基	NAA/（mg·L⁻¹）	IBA/（mg·L⁻¹）
A₁	3	3	B₁	1.0	0.1	0.02	C₁	1/2 MS	0.1	0.01
A₂	3	6	B₂	1.0	0.2	0.04	C₂	1/2 MS	0.2	0.05
A₃	3	9	B₃	1.0	0.4	0.06	C₃	1/2 MS	0.3	0.10
A₄	5	3	B₄	2.0	0.1	0.04	C₄	1/4MS	0.1	0.05
A₅	5	6	B₅	2.0	0.2	0.06	C₅	1/4MS	0.2	0.10
A₆	5	9	B₆	2.0	0.4	0.02	C₆	1/4MS	0.3	0.01
A₇	8	3	B₇	3.0	0.1	0.06	C₇	改良1/2 MS	0.1	0.10
A₈	8	6	B₈	3.0	0.2	0.02	C₈	改良1/2 MS	0.2	0.01
A₉	8	9	B₉	3.0	0.4	0.04	C₉	改良1/2 MS	0.3	0.05

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表 2 外植体不同消毒处理的消毒效果

Table 2. Comparison of different disinfection treatment

消毒处理代号 Disinfection treatment code	无菌率 Sterility rate/%	存活率 Survival rate/%	消毒效果 Disinfection effect
A₁	19.44±1.85 Gg	97.37±0.58 Aa	0.19±0.02 Ff
A₂	25.56±2.96 FGfg	96.17±0.44 ABab	0.25±0.03 Ee
A₃	45.00±6.67 Ee	93.67±1.31 Bb	0.42±0.06 Dd
A₄	57.78±2.96 Dd	89.73±0.71 Cc	0.52±0.03 Cc
A₅	61.67±1.11 CDcd	84.20±1.00 DEde	0.52±0.02 Cc
A₆	67.78±1.85 Cc	83.33±1.56 Ee	0.56±0.01 Cc
A₇	78.89±1.85 Bb	85.53±2.62 Dd	0.67±0.02 ABab
A₈	87.22±0.74 Aa	80.37±0.64 FGfg	0.70±0.01 Aa
A₉	82.22±0.74 ABab	77.80±1.73 Gg	0.64±0.02 Bb
同列数据后不同大写和小写字母分别表示1%和5%显著水平。表3同。 The uppercas and lowercse letters represent 1% and 5% significant level, respectively.The same as Table 3.

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表 3 芽苗增殖试验和生根配方试验结果

Table 3. Multiple comparison of shoot proliferation test and rooting formula test

芽苗增殖 Shoot proliferation test			生根配方 Rooting formula test
处理代号 Treatment code	增殖系数 Multiplication multiple	芽苗长势 Bud and seedling growth	处理代号 Processing code	生根率 Rooting rate/%	根系状况 Root condition
B₁	1.90±0.03 Gg	芽苗少，长势较弱	C₁	47.78±5.19 Ee	根细、较长
B₂	2.03±0.11 Ff	芽苗较少，长势一般	C₂	60.56±1.85 Dd	根细、少
B₃	2.37±0.13 Ee	芽苗较少，长势良好	C₃	71.11±4.07 Cc	基部愈伤膨大，根粗短
B₄	3.07±0.13 Cc	芽苗分化多，长势健壮	C₄	61.11±2.59 Dd	根细
B₅	3.47±0.18 ABab	芽苗分化多，长势健壮	C₅	80.56±0.74 Bb	根细而长
B₆	3.55±0.16 Aa	芽苗分化多，长势较弱	C₆	72.78±1.85 Cc	基部愈伤大，根短粗
B₇	3.22±0.08 BCbc	芽苗分化多，长势一般	C₇	73.33±1.11 Cc	根细长，量少
B₈	2.72±0.11 Dd	芽苗较多，长势较弱	C₈	89.44±1.85 Aa	根健壮
B₉	2.54±0.09 DEde	芽苗较多，长势较弱	C₉	90.56±2.59 Aa	基部愈伤膨大，根粗短

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参考文献(8)

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