西班牙河碳酸盐岩对茶园土壤状况和土壤细菌群落的影响

赵茜; 施龙清; 黄世勇; 丁鲁平; LietteVasseur; 杨广

doi:10.19303/j.issn.1008-0384.2020.09.014

西班牙河碳酸盐岩对茶园土壤状况和土壤细菌群落的影响

doi: 10.19303/j.issn.1008-0384.2020.09.014

赵茜^{1, 2, 3, 4,},
施龙清^{1, 5},
黄世勇⁶,
丁鲁平⁷,
LietteVasseur⁸,
杨广^{1, 2, 3, 4, ,}

1.
闽台作物有害生物生态防控国家重点实验室/福建农林大学应用生态研究所，福建　福州　350002
2.
福建农林大学教育部害虫生态防控国际合作联合实验室，福建　福州　350002
3.
农业农村部闽台作物有害生物综合治理重点实验室，福建福州350002
4.
害虫绿色防控福建省高等学校重点实验室，福建　福州　350002
5.
福建省农业科学院水稻研究所，福建　福州　350019
6.
武夷山市种子站，福建　武夷山　354300
7.
博莱生态农业科技有限公司，北京　100000
8.
加拿大布鲁克大学生物科学系，安大略圣凯瑟琳斯　L2S3A1

基金项目: 政府间国际科技创新合作重点专项（2016YFE0102100）；闽台作物有害生物生态防控国家重点实验室开放课题基金（SKL2018005）；福建农林大学茶产业链科技创新与服务体系建设项目（K1520007A03）

详细信息

作者简介:
赵茜（1986−），女，博士，助理研究员，研究方向：生态农业（E-mail：zhaoqian977228@126.com)

通讯作者:
杨广（1973−），男，教授，研究方向：生态农业（E-mail：yxg@fafu.edu.cn)

中图分类号: S 154
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- 被引次数: 0
出版历程
- 收稿日期: 2020-06-10
- 修回日期: 2020-07-14
- 刊出日期: 2020-09-28

Effects of Spanish River Carbonatite on Soil and Bacterial Community at Tea Plantations

ZHAO Qian^{1, 2, 3, 4
,},
SHI Longqing^{1, 5},
HUANG Shiyong⁶,
DING Luping⁷,
Liette Vasseur⁸,
YANG Guang^{1, 2, 3, 4
, ,}

1.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, Fujian　350002, China
3.
Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fuzhou, Fujian　350002, China
4.
Key Laboratory of Green Pest Control for Universities in Fujian Province, Fuzhou, Fujian　350002, China
5.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350019, China
6.
Wuyishan Seed Station, Wuyishan, Fujian　354300, China
7.
The Bolai Eco-Agriculture Technology Co., Ltd., Beijing 10000, China
8.
Department of Biological Sciences, Brock University, St. Catharines, ON　L2S3A1, Canada

摘要

摘要: 目的探讨西班牙河碳酸盐岩（Spanish river carbonatite, SRC）对茶园土壤酸化、重金属以及土壤细菌多样性和群落结构的影响，为茶园土壤改良提供科学依据。方法通过盆栽和大田试验，应用pH计、原子荧光法以及高通量测序技术分析施用SRC对茶园土壤pH、重金属含量以及细菌群落的影响。结果大田和盆栽试验均表明添加SRC可提高茶树种植土壤的pH值，盆栽试验表明低浓度的SRC的施入量就可显著降低土壤中Cd、Cu的含量，大田试验表明SRC的施用可显著降低8种重金属的含量；盆栽试验表明SRC对土壤微生物的组成有显著影响，大田试验表明施用SRC可提高土壤养分。结论施用SRC可显著改良茶园土壤，改良土壤重金属污染，增加茶园土壤的养分。
- 西班牙河碳酸盐（SRC） /
- 茶园 /
- 土壤酸化 /
- 土壤重金属 /
- 细菌多样性 /
- 细菌群落结构
Abstract: Objective Effects of Spanish river carbonatite (SRC) on acidification and metal elements in soil and structure and diversity of microbial community at tea plantations were investigated. Method Changes on pH, heavy metals, and microbial community in the soil under SRC application were monitored for evaluation. Result The field and pot culture experiments showed that SRC addition alleviated acidification and heavy mental pollution in the soil. In the pot culture, low concentration of SRC significantly reduced the contents of Cd and Cu in soil. Whereas, the field experiment showed significant reductions on all tested heavy metals. In comparison to control, SRC addition exerted a significant impact on the microbial community structure in the pot culture, and increased the nutrient contents facilitating the microbial growth in the field test. Conclusion Both field and pot experiments demonstrated the desirable rise on pH, decline on heavy metals content, and improvements on microbial diversity and community structure in the soil by the incorporation of SRC.
- Spanish river carbonatite /
- tea plantation /
- soil acidification /
- soil heavy mental /
- bacterial diversity /
- bacterial community structure

HTML全文

图 1 室内SRC处理土壤细菌群落种类分布及主成分分析

Figure 1. PCA on species distribution of microbial community in pot soils

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图 2 基于UPGMA算法的土壤细菌聚类分析

Figure 2. UPGMA-based cluster analysis on soil microbes

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图 3 茶园SRC处理土壤细菌群落种类分布及主成分分析（PCA）

Figure 3. PCA on species distribution of microbial community in soils at tea plantations

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表 1 室内SRC处理对土壤pH值及重金属含量的影响

Table 1. Effects of SRC on pH and heavy metals in pot soil

指标 Index	CK（2017）	CK（2018）	1%SRC	5%SRC	10%SRC	20%SRC
pH	6.13±0.09 b	5.93±0.09 b	6.23±0.19 ab	6.10±0.06 b	6.60±0.54 a	6.10±0.15 b
Cd/（mg·kg⁻¹）	0.29±0.02 b	0.35±0.034 ab	0.21±0.01 c	0.21±0.02 c	0.38±0.02 a	0.11±0.001 c
Cr/（mg·kg⁻¹）	40.30±1.55 b	54.20±4.84 a	51.70±1.24 a	55.40±2.66 a	50.70±0.29 a	39.90±3.89 b
Pb/（mg·kg⁻¹）	29.10±2.02 c	39.10±4.05 ab	33.00±0.75 b	45.60±1.12 a	34.70±3.07 b	25.50±1.70 c
Cu/（mg·kg⁻¹）	116.70±2.90 b	104.70±9.60 b	220.80±13.50 a	221.50±12.20 a	65.50±4.80 c	30.60±2.20 d
Ni/（mg·kg⁻¹）	18.70±0.88 b	36.80±3.77 a	32.20±1.09 a	34.40±2.03 a	32.80±3.13 a	17.60±2.50 b
Zn/（mg·kg⁻¹）	149.00±7.05 b	263.30±31.80 a	227.70±8.37 a	237.20±11.00 a	232.00±27.20 a	80.90±4.09 c
Hg/（mg·kg⁻¹）	0.05±0.01 c	0.04±0.001 c	0.09±0.01 b	0.09±0.01 b	0.04±0.00 c	0.13±0.00 a
As/（mg·kg⁻¹）	4.85±0.23 b	3.82±0.37 c	6.17±0.04 a	6.60±0.24 a	3.82±0.37 c	2.58±0.24 d
注：同行数据后不同小写字母表示处理间差异显著（P＜0.05）。表3同。 Note: Data with different lowercase letters indicate significant difference among treatments at P＜0.05. The same as Table 3.

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表 2 室内SRC处理对土壤细菌多样性的影响

Table 2. Effects of SRC on microbial diversity in pot soil

样本 Samples	Simpson	Chao1	ACE	Shannon
CK（2017）	0.994 b	1 072.0 b	1 147.4 b	8.91 b
CK（2018）	0.997 a	1 223.8 ab	1 339.8 ab	9.32 a
1%SRC	0.997 a	1 323.0 a	1 451.7 a	9.36 a
5%SRC	0.996 a	1 208.7 ab	1 333.1 ab	9.17 ab
10%SRC	0.997 a	1 264.1 a	1 404.5 a	9.28 a
20%SRC	0.997 a	1 334.3 a	1 464.7 a	9.38 a
注：同列数据后不同小写字母表示差异显著（P ＜ 0.05）。表4同。 Note: Data with different lowercase letters indicate significant difference among treatments at P＜0.05. The same as Table 4.

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表 3 SRC处理对茶园土壤pH值和重金属含量的影响

Table 3. Effects of SRC on pH and heavy metals in soils at tea plantations

指标 Index	CK 2018	CK 2019	SRC
pH	5.40±0.12 b	5.49±0.25 b	6.23±0.14 a
Cd/（mg·kg⁻¹）	0.16±0.01 a	0.17±0.01 a	0.10±0.01 b
Cr/（mg·kg⁻¹）	74.60±4.73 ab	78.30±5.31 a	63.30±2.65 b
Pb/（mg·kg⁻¹）	88.00±4.57 ab	102.10±10.41 a	82.20±2.90 b
Cu/（mg·kg⁻¹）	126.40±4.30 a	149.40±11.26 a	97.30±5.19 b
Ni/（mg·kg⁻¹）	83.20±4.16 a	84.90±10.45 a	68.18±6.13 b
Zn/（mg·kg⁻¹）	320.80±8.64 a	307.30±24.91 a	159.60±14.20 b
Hg/（mg·kg⁻¹）	0.06±0.01 a	0.06±0.01 a	0.04±0.01 b
As/（mg·kg⁻¹）	8.15±0.30 a	9.46±0.48 a	5.89±0.95 b

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表 4 SRC处理对茶园土壤细菌多样性的影响

Table 4. Effects of SRC on microbial diversity in soils at tea plantations

样本 Sample	Simpson	Chao1	Shannon
CK 2018	0.954 a	482.5 c	6.33 c
CK 2019	0.972 a	1699.7 b	7.64 b
SRC	0.997 a	3077.0 a	9.89 a

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