氮磷添加对何首乌生长和叶绿素荧光参数的影响

刘筱; 韩燕峰; 杨淑君; 姬拉拉; 王锐洁; 江学海; 王健健

doi:10.19303/j.issn.1008-0384.2019.02.004

氮磷添加对何首乌生长和叶绿素荧光参数的影响

doi: 10.19303/j.issn.1008-0384.2019.02.004

1.
贵州大学生命科学学院, 贵州贵阳 550025
2.
贵州省农业科学院水稻研究所, 贵州贵阳 550025

基金项目:

国家自然科学基金项目 31760155

贵州省自然科学基金项目 2016（1033）

贵州省农业领域科技成果转化重点项目 [2015]5021号

详细信息

作者简介:
刘筱(1994-), 女, 硕士研究生, 研究方向:植物生理生态(E-mail:1414943118@qq.com)

通讯作者:
王健健(1985-), 男, 博士, 副教授, 研究方向:植物生理生态(E-mail:wangjj33209@163.com)

中图分类号: S567.21
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- 被引次数: 0
出版历程
- 收稿日期: 2018-12-24
- 修回日期: 2019-02-02
- 刊出日期: 2019-02-28

Effects of Nitrogen and Phosphorus Additions on Growth and Chlorophyll Fluorescence Indices of Polygonum multiflorum

1.
College of Life Sciences Guizhou University, Guiyang, Guizhou 550025, China
2.
Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550025, China

摘要

摘要: 目的研究不同氮磷添加处理对何首乌植株生长和叶绿素荧光参数的影响，为促进何首乌生产提供科学施肥的理论指导。方法以何首乌幼苗为试材，设置4个处理，分别为对照（CK）、施氮（N：株施尿素2.7 g）、施磷（P：株施过磷酸钙14.4 g）、氮磷共施（N+P：株施尿素2.7 g、过磷酸钙14.4 g），测定不同氮磷添加处理对何首乌生长和叶绿素荧光参数的影响。结果氮磷添加处理可促进何首乌生长，对何首乌叶片数、单叶面积、叶柄长、株高的影响程度为：氮磷共施>施磷>施氮>对照；与对照相比，氮磷添加处理均可显著促进何首乌生物量积累，以氮磷共施处理增幅最大，并可显著提高地上生物量占比；氮磷添加处理促进了何首乌叶绿素含量增加，以及Fv/Fm、qP、ETR值升高，降低qN值，以氮磷共施处理的叶绿素含量增幅最大（较对照增加96.08%），qN值降幅最大（较对照降低29.16%）。结论相较于对照及单一养分添加，氮磷共施处理能更显著提高何首乌光合效率，促进植株生长。
- 何首乌 /
- 氮磷添加 /
- 生长 /
- 叶绿素荧光参数
Abstract: Objective Effects of N and P additions on the growth and chlorophyll fluorescence indices of Polygonum multiflorum were studied to provide a guideline for proper fertilization to promote the plant growth. Method N and P addition, i.e., (1) N application using 2.7g urea/plant, (2) P application using 14.4g superphosphate/plant, (3) N+P application using 2.7 g urea and 14.4 g superphosphate on each plant, or (4) control without added N or P, was incorporated in the potting soil to observe the resulting growth and chlorophyll fluorescence indices of the plants. Result The treatments increased the leaf count, single leaf area, petiole length, and height of the plants in the order of N+P application > P application > N application > control. The addition of N+P significantly increased the biomass accumulation, proportion of above-ground biomass, and chlorophyll content as well as the indices of Fv/Fm, qP and ETR, with a decreased qN. Compared to control, N+P treatment affected the most on chlorophyll with a 96.08% increase and on qN with a 29.16% reduction. Conclusion Addition of both N and P in the potting soil significantly promoted the growth and photosynthesis of P. multiflorum.
- Polygonum multiflorum /
- nitrogen and phosphorus addition /
- growth /
- chlorophyll fluorescence indices

HTML全文

图 1 不同氮磷添加处理的何首乌叶绿素含量

注:柱形图间无相同大、小写字母者分别表示在0.01水平上和0.05水平上具有显著性差异，图 2同。

Figure 1. Chlorophyll content of P. multiflorum seedlings under N and P applications

Note:Different uppercase letters between columns indicate extremely significant differences at 0.01 level. Different lowercase letters between columns indicate significant differences at 0.05 level.The same as fig. 2.

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图 2 不同氮磷添加处理的何首乌叶绿素荧光参数

Figure 2. Chlorophyll fluorescence indices of P. multiflorum seedlings under N and P applications

下载: 全尺寸图片幻灯片

表 1 不同氮磷添加处理何首乌幼苗的生长指标

Table 1. Growth indices of P. multiflorum seedlings under N and P applications (mean±standard error)

处理 Nutrient treatment	叶片数Number of leaves	叶面积 Single leaf area/cm²	叶柄长 Petiole length/cm	根长 Root length/cm	分枝数 Number of branches	株高 Plant height/cm
CK	36.67±4.98cB	9.16±0.58cB	2.1±0.13bA	18.10±1.47aA	7.0±1.53aA	77.7±5.37cC
N	63.33±6.17bAB	13.76±0.47bB	2.5±0.25abA	20.77±3.75aA	6.0±1.0aA	106.3±16.95bcBC
P	72.00±6.43bB	16.11±0.24bAB	3.1±0.27aA	17.10±1.15aA	6.7±0.67aA	141.7±7.26bB
N+P	133.33±21.37aA	34.57±7.85aA	3.3±0.20aA	18.53±0.67aA	6.7±1.76aA	312.8±13.42aA
注:表中同列数据后无相同大、小写字母者分别表示在0.01水平上和0.05水平上具有显著性差异。表 2同。 Note:Different uppercase and lowercase letters on a same column indicate significant differences at 0.05 level and extremely significant differences at 0.01 level. The same as table 2.

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表 2 不同氮磷添加处理何首乌幼苗生物量积累及其分配

Table 2. Biomass accumulation and allocation on P. multiflorum seedlings under N and P applications (mean±standard error)

处理 Nutrient treatment	叶生物量 Leaf biomass/g	块根生物量 Root biomass/g	须根生物量 Fine root biomass/g	茎生物量 Stem biomass/g	总生物量 Total biomass/g	叶生物量占比 Leaf biomass ratio	茎生物量占比 Stem biomass ratio	根生物量占比 Root biomass ratio
CK	1.2±0.19cB	1.1±0.09bB	0.12±0.02aA	0.9±0.14bB	2.9±0.57cC	0.2±0.1bA	0.3±0.02aA	0.4±0.05aA
N	2.3±0.03bcB	2.1±0.25aA	0.38±0.03bB	1.1±0.42bB	5.8±0.68bBC	0.4±0.05abA	0.2±0.06bB	0.4±0.01aAB
P	3.4±0.03bB	2.0±0.11aA	0.29±0.10bB	2.1±0.27bB	7.9±0.49bB	0.4±0.2abA	0.3±0.02abAB	0.3±0.0aAB
N+P	7.4±1.11aA	2.0±0.09aA	0.47±0.23bB	5.4±0.48aA	15.3±1.23aA	0.5±0.05aA	0.4±0.04aA	0.2±0.01bA

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