Effects of Light Exposure on Cell Culture and Corilagin Synthesis of Longan
-
摘要:
目的 研究不同光照条件对龙眼胚性悬浮细胞培养及柯里拉京代谢合成的影响，以期为龙眼胚性悬浮细胞大规模培养、工业化生产柯里拉京奠定理论基础。 方法 基于龙眼胚性悬浮细胞体系的建立，采用5 L新型搅拌式生物反应器放大培养龙眼胚性悬浮细胞，探讨黑暗和光照处理对龙眼胚性悬浮细胞生长、柯里拉京含量、蔗糖消耗以及培养体系中pH和DO（溶氧）的动态变化规律。 结果 龙眼胚性悬浮细胞在黑暗和光照条件下其生长动态大致均呈“S”形曲线，最大增重分别为4.74 g·L-1和3.71 g·L-1；黑暗培养到第7 d龙眼胚性悬浮细胞体内柯里拉京含量达到最大值8.72 mg·g-1，是光照条件下的2.8倍，但在细胞培养液中柯里拉京含量明显低于光照条件（P < 0.05）。光照加快了龙眼胚性悬浮细胞对蔗糖的消耗速度，但对培养体系中的pH和DO无明显影响。 结论 生物反应器大规模培养过程中，黑暗条件利于龙眼胚性悬浮细胞生长及细胞内柯里拉京含量的积累，蔗糖消耗、pH和DO是龙眼胚性悬浮细胞生长及柯里拉京合成的重要调控因子。 Abstract:Objective Effects of light exposure on embryogenic cell and corilagin formations of longan (Dimocarpus longan Lour.) in a suspension culture were investigated to provide theoretical reference for the large-scale operation of longan embryogenic cell culture and industrial production of corilagin. Method A 5 L stirring bioreactor was used in the experimentation. Dynamic changes on the cell growth, corilagin content, substrate consumption, pH, and dissolved oxygen (DO) in the medium under dark or light condition were monitored. Result The cellular growth curve showed an S shape with a peaked weight of 4.74 g·L-1 in the dark and 3.71 g·L-1 under the light. Cultured 7 days in the dark, the corilagin content in the longan cells reached a maximum of 8.72 mg·g-1, which was 2.8 times higher than that with the light exposure. But the corilagin in the medium was significantly less without than with the light exposure (P < 0.05). Light accelerated the sucrose consumption by the cells but had no significant effect on pH or DO in the medium. Conclusion For large-scale operations, the absence of light in an enclosed reactor would facilitate the embryogenic growth and corilagin accumulation. Meanwhile, process controls on sucrose, pH and DO were also important as these conditions regulated the cell growth and corilagin formation as well. -
Key words:
- Dimocarpus longan Lour. /
- cell culture /
- bioreactor /
- light exposure /
- corilagin
-
图 3 龙眼胚性悬浮细胞培养液中蔗糖消耗量的变化
注：蔗糖消耗量(g·L-1)=20-培养液中蔗糖含量。
Figure 3. Sucrose consumption in medium during longan embryonic cell culture
Note:Sucrose consumption(g·L-1)=20-sucrose content in culture medium.
表 1 生物反应器放大培养龙眼胚性悬浮细胞相关参数
Table 1. Process conditions of longan embryonic cells suspension culture in bioreactor
培养参数
Parameters参数值
Value温度Temperature 25±2℃ 初始pH Initial pH 5.8 搅拌转速Speed of stirring 120 r·min-1 接种密度Inoculum density 40 g·L-1FW 通气量Ventilation 100 cm3·min-1 初始液位Initial level 4 L 培养基Medium MS+20 g·L-1蔗糖+1.0 mg·L-1
2, 4-D+100 mg·L-1肌醇培养天数Cultured day 9 d 光照强度(白光)Intensity of white light 32 μmol·m-2·s-1 光照周期(白光)Photoperiod of white light 12 h 
下载: 导出CSV
表 2 龙眼胚性悬浮细胞培养液中柯里拉京的含量变化
Table 2. Changes on corilagin content in suspension medium for longan embryonic cell culture
培养天数
Cultured day/d柯里拉京含量Corilagin content/(mg·mL-1) 黑暗Dark 光照Light 1 ND ND 2 ND ND 3 ND (0.56±0.03)aA 4 (0.15±0.03)bB (0.84±0.05)aA 5 (0.17±0.04)bB (0.87±0.05)aA 6 (0.32±0.02)bA (0.44±0.05)aA 7 ND (0.27±0.03)aA 8 ND (0.18±0.03)aA 9 ND ND 注：ND代表UPLC未检测出细胞培养液中柯里拉京含量；不同大、小写字母分别表示差异极显著(P < 0.01)或差异显著(P < 0.05)。
Note：ND indicates that corilagin was not detected in culture medium by UPLC. Different uppercase letters represent highly significant difference at P < 0.01, and lowercase letters significant difference at P < 0.05.
下载: 导出CSV
-
[1] 郑公铭, 魏孝义, 徐良雄, 等.龙眼果皮化学成分的研究[J].中草药, 2011, 42(8):1485-1489. http://d.old.wanfangdata.com.cn/Periodical/hbnykx201503046ZHENG G M, WEI X Y, XU L X, et al.Chemical constituents from pericarp of longan fruits[J]. Chinese Traditional and Herbal Drugs, 2011, 42(8):1485-1489.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hbnykx201503046 [2] SOONG Y Y, BARLOW P J.Isolation and structure elucidation of phenolic compounds from longan (Dimocarpus longan Lour.) seed by high-performance liquid chromatography-electrospray ionization mass spectrometry[J]. Journal of Chromatography A, 2005, 1085(2):270-277. doi: 10.1016/j.chroma.2005.06.042 [3] 陈一燕, 陈崇宏.柯里拉京药理活性研究进展[J].中国现代应用药学, 2010, 27(5):390-394. http://d.old.wanfangdata.com.cn/Periodical/zgbjyy-kp201712606CHEN Y Y, CHEN C H.Advances in the Pharmacological Activity Research on Corilagin[J]. Chinese Journal of Modern Applied Pharmacy, 2010, 27(5):390-394.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgbjyy-kp201712606 [4] SHIOTA S, SHIMIZU M, SUGIYAMA J, et al.Mechanisms of action of corilagin and tellimagrandini that remarkably potentiate the activity of β-lactams against methicillin-resistant staphylococcus aureus[J]. Microbiology & Immunology, 2013, 48(1):67-73. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_PM14734860 [5] 张树峰, 明艳林, 林毅, 等.叶下珠属植物活性成分柯里拉京研究进展(综述)[J].亚热带植物科学, 2010, 39(4):79-83. doi: 10.3969/j.issn.1009-7791.2010.04.020ZHANG S F, MING Y L, LIN Y, et al.Research progress on Corilagin of Phyllanthus[J]. Subtropical Plant Science, 2010, 39(4):79-83.(in Chinese) doi: 10.3969/j.issn.1009-7791.2010.04.020 [6] JIA L, ZHOU J, ZHAO H, et al.Corilagin sensitizes epithelial ovarian cancer to chemotherapy by inhibiting Snail glycolysis pathways[J]. Oncology Reports, 2017, 38(4):2464-2470. doi: 10.3892/or.2017.5886 [7] 袁永兵, 张兰珍, 郭亚健, 等.RP-HPLC法测定叶下珠中没食子酸、柯里拉京和鞣花酸的含量[J].北京中医药大学学报, 2009, 32(1):56-58. doi: 10.3321/j.issn:1006-2157.2009.01.016YUAN Y B, ZHANG L Z, GUO Y J, et al.Quantitative determination of gallic acid, corilagin and ellagic acid inPhyllanthus Urinaria by RP-HPLC[J]. Journal of Beijing University of Traditional Chinese Medicine, 2009, 32(1):56-58.(in Chinese) doi: 10.3321/j.issn:1006-2157.2009.01.016 [8] 李克明, 张永文, 陈玉武.不同采收月份老鹳草生药中柯里拉京的含量比较[J].中成药, 2004, 26(10):819-821. doi: 10.3969/j.issn.1001-1528.2004.10.014LI K M, ZHANG Y W, CHEN Y W.Comparison of corilagin content in Erodium stephanianum from different collection month[J]. Chinese Traditional Patent Medicine, 2004, 26(10):819-821.(in Chinese) doi: 10.3969/j.issn.1001-1528.2004.10.014 [9] 胡涛, 吕春茂, 王新现, 等.植物细胞培养生物反应器研究进展[J].安徽农业科学, 2010, 38(4):1702-1703. doi: 10.3969/j.issn.0517-6611.2010.04.017HU T, LU C M, WANG X X, et al.Research Progress in Bioreactor for Plant Cell Culture[J]. Journal of Anhui Agricultural Sciences, 2010, 38(4):1702-1703.(in Chinese) doi: 10.3969/j.issn.0517-6611.2010.04.017 [10] 陈月华, 朱艳, 张翔, 等.植物细胞悬浮培养中次生代谢产物积累的研究进展[J].中国野生植物资源, 2016, 35(3):41-47. doi: 10.3969/j.issn.1006-9690.2016.03.011CHEN Y H, ZHU Y, ZHANG X, et al.Research Progress in Plant Cell Suspension Culture for Production of Secondary Metabolites[J]. Chinese Wild Plant Resources, 2016, 35(3):41-47.(in Chinese) doi: 10.3969/j.issn.1006-9690.2016.03.011 [11] 谷荣辉, 洪利亚, 龙春林.植物细胞培养生产次生代谢物的途径[J].植物生理学报, 2013, 49(9):869-881. http://d.old.wanfangdata.com.cn/Periodical/whhgxyxb200501008GU R H, HONG L Y, LONG C L.The Ways of Producing Secondary Metabolites via Plant Cell Culture[J]. Plant Physiology Journal, 2013, 49(9):869-881.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/whhgxyxb200501008 [12] 刘生财, 段俊朋, 徐小萍, 等.真菌诱导子对龙眼胚性愈伤组织中类黄酮、类胡萝卜素和单宁含量的影响[J].热带作物学报, 2018, 39(9):1778-1785. doi: 10.3969/j.issn.1000-2561.2018.09.015LIU S C, DUAN J P, XU X P, et al.Effects of Fungal Elicitors on the Content of Flavonoids, Carotenoids and Alkaloids in Embryogenic Calli of Dimocarpus longan Lour[J]. Chinese Journal of Tropical Crops, 2018, 39(9):1778-1785.(in Chinese) doi: 10.3969/j.issn.1000-2561.2018.09.015 [13] 刘永刚.雪莲细胞培养及其次生代谢产物黄酮的代谢调控[D].北京: 中国农业大学, 2005. http://cdmd.cnki.com.cn/Article/CDMD-10019-2005084186.htmLIU Y G.Saussurea involucrata Cell Culture and Secondary Metabolic Regulation of Flavonoids[D]. Beijing: China Agricultural University, 2005.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10019-2005084186.htm [14] 李雅丽, 孟婷婷, 王毛毛, 等.甘草细胞在搅拌式生物反应器中的放大培养[J].植物生理学报, 2015(3):302-306. http://cdmd.cnki.com.cn/Article/CDMD-10487-1013011080.htmLI Y L, MENG T T, WANG M M, et al.Amplifi cation Culture of Glycyrrhiza uralensis Cell in Stirring Bioreactor[J]. Plant Physiology Journal, 2015(3):302-306.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10487-1013011080.htm [15] ZORATTI L, KARPPINEN K, ESCOBAR A, et al.Light-controlled flavonoid biosynthesis in fruits[J]. Frontiers in Plant Science, 2014, 5(5):534. http://www.ncbi.nlm.nih.gov/pmc/articles/pmc4191440/ [16] 谷艾素, 张欢, 崔瑾.光调控在植物组织培养中的应用研究进展[J].西北植物学报, 2011, 31(11):2341-2346. http://d.old.wanfangdata.com.cn/Periodical/xbzwxb201111033GU A S, ZHANG H, CUI J.Research Advances of Photoregulation and Its Utilization in Plant Tissue Culture[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(11):2341-2346.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xbzwxb201111033 [17] 范美华.半夏细胞悬浮培养和次生代谢物提取的初步研究[D].武汉: 华中师范大学, 2004. http://cdmd.cnki.com.cn/Article/CDMD-10511-2005030617.htmFAN M H.Primary Study on Cell Suspension Cultureand Secondary Metabolize Production of Pinellia ternata(Thunb.)Briet[D]. Wuhan: Central China Normal Univerity, 2004.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10511-2005030617.htm [18] 王娟, 高文远, 黄滔, 等.培养条件对西洋参悬浮细胞生物量和活性成分的影响[J].中国中药杂志, 2009, 34(4):375-378. doi: 10.3321/j.issn:1001-5302.2009.04.001WANG J, GAO W Y, HUANG T, et al.Effects of culture conditions on biomass and active components of suspension cells of Panax quinquefolium[J]. China Journal of Chinese Materia Medica, 2009, 34(4):375-378.(in Chinese) doi: 10.3321/j.issn:1001-5302.2009.04.001 [19] 陈顺钦, 袁媛, 罗毓健, 等.光照对黄芩黄酮类活性成分积累及其相关基因表达的影响[J].中国中药杂志, 2010, 35(6):682-685. http://d.old.wanfangdata.com.cn/Periodical/zgzyzz201006004CHEN S Q, YUAN Y, LUO Y J, et al.Effects of light on flavonoids accumulation and related gene expression in suspension cultures of Scutellaria baicalensis[J]. China Journal of Chinese Materia Medica, 2010, 35(6):682-685.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgzyzz201006004 [20] 李春斌, 王关林, 岳玉莲, 等.培养条件对银杏悬浮培养细胞黄酮合成影响研究[J].大连理工大学学报, 2003, 43(3):287-291. doi: 10.3321/j.issn:1000-8608.2003.03.008LI C B, WANG G L, YUE Y L, et al.Research on effectof culture condition factors on synthesis of flavone glycosides in cell suspension of Ginkgo biloba L.[J]. Journal of Dalian University of Technology, 2003, 43(3):287-291.(in Chinese) doi: 10.3321/j.issn:1000-8608.2003.03.008 [21] 杨蕾.丹参中丹参酮类物质的生物合成及其调控研究进展[J]. Botanical Research, 2013, 2(3):73-78. http://cdmd.cnki.com.cn/Article/CDMD-10338-1015330147.htmYANG L.Biosynthesis of Tanshinone and Its Regulation in Salvia miltiorrhiza[J]. Botanical Research, 2013, 2(3):73-78.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10338-1015330147.htm [22] 王蒂, 陈劲枫.植物组织培养[M].第2版.中国农业出版社, 2013.WANG D, CHEN J F.Plant Tissue Culture[M]. The second edition.China Agriculture Press, 2013.(in Chinese) [23] 李琰, 冯俊涛, 王永宏, 等.培养基及培养条件对雷公藤愈伤组织生长和次生代谢产物含量的影响[J].林业科学, 2010, 46(5):64-69. http://d.old.wanfangdata.com.cn/Periodical/lykx201005011LI Y, FENG J T, WANG Y H, et al.Effects of Basic Media and Culture Conditions on Callus Growth and Secondary Metabolite Content of Tripterygium wilfordii[J]. Scientia Silvae Sinicae, 2010, 46(5): 64-69.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/lykx201005011 [24] 林小苹, 赖钟雄, 黄浅.不同光质对龙眼胚性愈伤组织生长和细胞膜保护酶活性的影响[J].福建农林大学学报(自然科学版), 2008, 37(3):253-256. http://d.old.wanfangdata.com.cn/Periodical/fjnydxxb200803007LIN X P, LAI Z X, HUANG Q.Effect of light quality on the growth, cell membrane protection enzyme activities of embryogenic calli in longan[J]. Journal of Fujian Agriculture and Forestry University(Natural Science Edition), 2008, 37(3):253-256.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/fjnydxxb200803007 [25] 董慧雪, 周燕蓉, 田奇琳, 等.不同光质对龙眼胚性愈伤组织类黄酮含量的影响[J].热带作物学报, 2014, 35(12):2374-2377. doi: 10.3969/j.issn.1000-2561.2014.12.010DONG H X, ZHOU Y R, TIAN Q L, et al.Effects of Different Light Qualities on the Contents of Flavonoids of Embryonic Callus in Dimocarpus longan Lour[J]. Chinese Journal of Tropical Crops, 2014, 35(12):2374-2377.(in Chinese) doi: 10.3969/j.issn.1000-2561.2014.12.010 [26] 李汉生, 姚德恒, 陈晓慧, 等.蓝光对龙眼细胞培养及类黄酮代谢的影响[J].热带作物学报, 2018, 39(4):694-701. doi: 10.3969/j.issn.1000-2561.2018.04.013LI H S, YAO D H, CHEN X H, et al.Effects of Blue Light on the Cultivation of Longan Cells and Flavonoid Metabolism[J]. Chinese Journal of Tropical Crops, 2018, 39(4):694-701.(in Chinese) doi: 10.3969/j.issn.1000-2561.2018.04.013 [27] 赖钟雄, 陈振光.龙眼胚性细胞悬浮培养再生植株[J].应用与环境生物学报, 2002, 8(5):485-491. doi: 10.3321/j.issn:1006-687X.2002.05.008LAI Z X, CHEN Z G.Plant Regeneration from Embryogenic Cell Suspensions VIA Somatic Embryogenesis of Longan (Dimocarpus Longan Lour.)[J]. Chinese Journal of Applied & Environmental Biology2002, 8(5):485-491.(in Chinese) doi: 10.3321/j.issn:1006-687X.2002.05.008 [28] KIM S J, HAHN E J, HEO J W, et al.Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro[J]. Scientia Horticulturae, 2004, 101(1):143-151. http://www.sciencedirect.com/science/article/pii/S0304423803001730 [29] 王莉, 史玲玲, 刘玉军.不同光质对长鞭红景天悬浮细胞生长及苯丙氨酸解氨酶活性的影响[J].林业科学, 2007, 43(6):52-56. http://d.old.wanfangdata.com.cn/Periodical/lykx200706009WANG L, SHI L L, LIU Y J.Effects of Different Light Treatments on Growth and PAL Activity of the Suspension-Cultured Cells of Rhodiola fastigiata[J]. Scientia Silvae Sinicae, 2007, 43(6):52-56.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/lykx200706009 [30] WANG F, LI G Y, CHEN S, et al.Callus Induction and Cell Suspension Culture of Eggplant(Solanum melongena L.)[J]. Agricultural Science and Technology, 2013, 14(9):1220-1223. http://en.cnki.com.cn/Article_en/CJFDTotal-HNNT201309005.htm [31] 赖钟雄, 潘良镇, 陈振光.龙眼胚性细胞系的建立与保持[J].福建农林大学学报(自然版科学), 1997(2):160-167. http://www.cnki.com.cn/Article/CJFDTOTAL-FJND702.006.htmLAI Z X, PAN L Z, CHEN Z G.Establishment and maintenance of longan embryogenic cell lines[J]. Journal of Fujian Agriculture and Forestry University(Natural Science Edition), 1997(2):160-167.(in Chinese) http://www.cnki.com.cn/Article/CJFDTOTAL-FJND702.006.htm [32] 钟春水, 赖钟雄, 赖瑞联, 等.金花茶松散型愈伤组织诱导与悬浮细胞系建立[J].热带作物学报, 2016, 37(3):476-481. doi: 10.3969/j.issn.1000-2561.2016.03.008ZHONG C S, LAI Z X, LAI R L, et al.Induction of Friable Callus and the Establishment of Suspension Cell Lines of Camellia nitidissima Chi[J]. Chinese Journal of Tropical Crops, 2016, 37(3):476-481.(in Chinese) doi: 10.3969/j.issn.1000-2561.2016.03.008 [33] 孥彦, 周晓东, 楼浙辉, 等.植物次生代谢产物及影响其积累的因素研究综述[J].江西林业科学, 2012(3):54-60. http://d.old.wanfangdata.com.cn/Periodical/jxlykj201203018NU Y, ZHOU X D, LOU Z H, et al.Review of Plant Secondary Metabolites and the Factors that Influence Its Accumulation[J]. Jiangxi Forestry Science and Technology, 2012(3):54-60.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jxlykj201203018 [34] 叶和春, 尹作鸿, 李目风, 等.不同理化因子对新疆紫草愈伤组织生长及紫草宁衍生物合成的影响[J].植物学报, 1991, 22(12):927- 931. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWXB199112007.htmYE H C, YIN Z H, LI M F, et al.Effects of Physical and Chemical Factors on Callus Growth and Shikonin Derivative Formation in the Callus Cultures of Arnebia Euchroma[J]. Journal of Integrative Plant Biology, 1991, 22(12):927- 931.(in Chinese) http://www.cnki.com.cn/Article/CJFDTOTAL-ZWXB199112007.htm [35] 王建华.人参愈伤组织无性系培养条件优化及次生代谢调控研究[D].长春: 吉林农业大学, 2006. http://cdmd.cnki.com.cn/article/cdmd-10193-2006160235.htmWANG J H.Studies on optimization of culture condition and second metabolism regulation of Panax ginseng[D]. ChangChun: Jilin Agricultural University, 2006.(in Chinese) http://cdmd.cnki.com.cn/article/cdmd-10193-2006160235.htm [36] 詹亚光, 宋继园, 王巍巍.暗培养对沙枣愈伤组织中原儿茶酸含量的影响[J].植物生理学报, 2007, 43(5):54. http://d.old.wanfangdata.com.cn/Periodical/zwslxtx200705053ZHAN Y G, SONG J Y, WANG W W.Effect of dark culture on the content of protocatechuic acid in the callus of Elaeagnus angustifolia L.[J]. Plant Physiology Journal, 2007, 43(5):54.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zwslxtx200705053 [37] PRICE J, LAXMI A, MARTIN S K S, et al.Global transcription profiling reveals multiple sugar signal transduction mechanisms in Arabidopsis[J]. Plant Cell, 2004, 16(8):2128-2150. doi: 10.1105/tpc.104.022616 [38] 陈俊伟, 谢鸣, 秦巧平.植物糖信号与激素信号之间的联系[J].植物生理学报, 2005, 41(3):279-285. http://d.old.wanfangdata.com.cn/Periodical/zwslxtx200503002CHEN J W, XIE M, QIN Q P.Connections of Sugar and Hormone Signaling in Plants[J]. Plant Physiology Journal, 2005, 41(3):279-285.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zwslxtx200503002 [39] SHIGETA J I, SATO K, MⅡ M.Effects of initial cell density, pH and dissolved oxygen on bioreactor production of carrot somatic embryos[J]. Plant Science, 1996, 115(1):109-114. doi: 10.1016/S0168-9452(96)04327-O [40] 刘家新.红豆杉细胞培养pH调控与生化反应器操作策略研究[D].天津: 天津大学, 1999. http://med.wanfangdata.com.cn/Paper/Detail?id=DegreePaper_Y283078LIU J X.Study on pH Manipulation BioreactorStrategies TaxusCell Culture[D]. Tianjin: Tianjin University, 1999.(in Chinese) http://med.wanfangdata.com.cn/Paper/Detail?id=DegreePaper_Y283078 [41] THANH N T, MURTHY H N, PAEK K Y.Optimization of ginseng cell culture in airlift bioreactors and developing the large-scale production system[J]. Industrial Crops and Products, 2014, 60:343-348. doi: 10.1016/j.indcrop.2014.06.036 [42] SMART N J, FOWLER M W.Effect of aeration on large-scale cultures of plant cells[J]. Biotechnology Letters, 1981, 3(4):171-176. doi: 10.1007-BF00239657/ [43] 许建峰, 刘传斌, 方晓丹, 等.高山红景天细胞悬浮培养中pH值对红景天甙胞外释放及细胞活性的影响[J].植物学报(英文版), 1997(11):1022-1029. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWXB199711006.htmXU J F, LIU C B, FANG X D, et al.Effects of medium pH Decrease on Salidroside Release and Cell Viability in Cell Suspension Culture of Rhodiola Sachalinensis[J]. Acta Botanica Sinica, 1997(11):1022-1029.(in Chinese) http://www.cnki.com.cn/Article/CJFDTOTAL-ZWXB199711006.htm [44] CAI Z, RIEDEL H, SAW N M M T, et al.Effects of elicitors and high hydrostatic pressure on secondary metabolism of Vitis vinifera suspension culture[J]. Process Biochemistry, 2011, 46(7):1411-1416. doi: 10.1016/j.procbio.2011.03.015 [45] 张东向, 李康, 姚娜, 等.DMSO和Tween-80对黄芩悬浮细胞系的影响[J].种子, 2007, 26(6):21-23. http://d.old.wanfangdata.com.cn/Periodical/zhongz200706006ZHANG D X, LI K, YAO N, et al.Effects of DMSO and Tween-80 on Cell Suspension Cultures of Scutellaria baicalensis Georgi[J]. Seed, 2007, 26(6):21-23.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zhongz200706006 -
点击查看大图
图(5) / 表(2)
计量
- 文章访问数: 1177
- HTML全文浏览量: 324
- PDF下载量: 54
- 被引次数: 0
