• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

酵母硒对产蛋高峰后期蛋鸡生化指标、抗氧化功能及脂质代谢的影响

Effects of yeast selenium on serum biochemical indexes, antioxidant function and lipid metabolism of laying hens in late laying peak period

    摘要
  • 摘要:
    目的 探讨酵母硒（selenium yeast, SY）对产蛋高峰后期蛋鸡血清生化指标、抗氧化功能及脂代谢的影响，并为SY在蛋鸡生产中的科学应用提供参考依据。
    方法 选取50周龄海兰褐蛋鸡448羽，随机分为4个处理组（每组7重复，每重复16羽），包括基础日粮组（硒0.092 mg·kg−1）与试验组（分别补充0.15、0.30、0.45 mg·kg−1 SY），构成梯度硒添加体系，实测硒含量为0.221、0.368和0.505 mg·kg−1。预试期10 d，正试期56 d，试验第56天采集血液及肝脏组织样品，检测抗生化指标、氧化能力、脂质代谢等相关指标。
    结果 与对照组相比，1）饲粮中添加0.30 mg·kg−1 SY显著提高血清碱性磷酸酶（alkaline phosphatase, ALP）活性，显著降低尿酸（uric acid, UA）含量（P＜0.05）。2）饲粮中添加不同水平SY均显著提高了血清中总抗氧化力（total antioxidant capacity, T-AOC）和核因子E2相关因子2（nuclear factor erythroid 2-related factor 2, Nrf2）的活性（P＜0.05）；3个试验组中，0.30和0.45 mg·kg−1 SY添加组血清还原型谷胱甘肽酶（reduced glutathione, GSH-Px）的活性均显著提升（P＜0.05），添加0.30 mg·kg−1 SY血清谷胱甘肽巯基-转移酶（glutathione S-transferase, GST）的活性也显著提高（P＜0.05）。添加0.30和0.45 mg·kg−1 SY显著增强了肝脏组织中GST和Nrf2的活性（P＜0.05）；0.45 mg·kg−1 组显著增强了GSH-Px和T-AOC的活性（P＜0.05）。3）SY添加组均能显著增强肝脏组织谷胱甘肽过氧化物酶2（glutathione peroxidase 2, GPX2）和谷胱甘肽过氧化物酶4（glutathione peroxidase 4, GPX4）的基因表达水平（P＜0.05）；当SY补充量达到0.30~0.45 mg·kg−1时，可进一步诱导谷胱甘肽过氧化物酶1（glutathione peroxidase 1, GPX1）mRNA的显著高表达（P＜0.05）；但饲粮中添加不同水平的SY对谷胱甘肽过氧化物酶3（glutathione peroxidase 3, GPX3）、超氧化物歧化酶1（superoxide dismutase 1, SOD1）和超氧化物歧化酶2（superoxide dismutase 2, SOD2）mRNA相对表达量均差异不显著（P＞0.05）。4）3个剂量组血清和肝脏脂质代谢相关指标胆固醇（total cholesterol, T-CHO）、甘油三脂（triglycerides, TG）、高密度脂蛋白胆固醇（cholesterol in high-density lipoproteins, HDL-C）、低密度脂蛋白胆固醇（cholesterol in low-density lipoproteins, LDL-C）含量差异均不显著（P＞0.05），仅0.45 mg·kg−1组肝脏HDL-C显著高于0.15、0.30 mg·kg−1组（P＜0.05）。
    结论 饲粮中添加0.30~0.45 mg·kg−1酵母硒可显著增强产蛋高峰后期蛋鸡的抗氧化防御能力，改善血清生化指标并上调部分抗氧化相关基因表达，从而有助于维持机体代谢稳态。

     

    Abstract:
    Objective Effects of supplementing feed with selenium-containing yeast (SY) on the serum biochemical indices, antioxidative function, and lipid metabolism of hens in late stage of peak egg production were studied.
    Method Four hundred forty eight 50-week-old Hy-Line Brown egg-laying hens were randomly divided into 4 groups, each comprising 7 replicates with 16 birds per replicate. Aside from a control group of the birds fed on a basal diet containing 0.092mg·kg−1 selenium, three treatment groups were given forage with added SY at 0.15, 0.30, and 0.45 mg·kg−1 for the gradient selenium supplementation in diet of 0.221, 0.368, and 0.505 mg·kg−1, respectively. After a 10d pre-test period, a trial lasting 56 d was executed. At the end of the trial, blood and liver tissue samples were collected from the hens for measurements of antioxidant activity, serum biochemistry, and lipid metabolism.
    Results Compared with control, (1) the addition of 0.30 mg·kg−1 SY significantly increased the serum alkaline phosphatase (ALP) and reduced the uric acid (P＜0.05) of the hens; (2) all SY supplementations significantly rose the total antioxidant capacity (T-AOC) and activity of nuclear factor erythroid 2-related factor 2 (Nrf2) in serum (P＜0.05), while the 0.30 mg·kg−1 and 0.45 mg·kg−1 additions resulted in significant increases on the serum reduced glutathione (GSH-Px) activity (P＜0.05), the added 0.30 mg·kg−1 SY yielded significantly higher activity of glutathione S-transferase (GST) in serum (P＜0.05), the 0.30 mg·kg−1 and 0.45 mg·kg−1 additions provided significantly enhanced activities of GST and Nrf2 in the liver (P＜0.05), and 0.45 mg·kg−1 SY gave significant rises on GSH-Px and T-AOC activities (P＜0.05); (3) the supplementations significantly upregulated the gene expressions of glutathione peroxidase 2 (GPX2) and GPX4 in the liver (P＜0.05), and when the SY addition reached 0.30–0.45 mg·kg−1, the mRNA expression of GPX1 was significantly upregulated (P＜0.05) but not those of GPX3, superoxide dismutase 1 (SOD1), and superoxide dismutase 2 (SOD2) (P＞0.05); (4) no significant differences in the serum and liver lipid metabolism indices, including the total cholesterol (T-CHO), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C), for all treatment groups were found (P＞0.05).
    Conclusion Dietary supplementation with 0.30-0.45mg·kg−1 SY significantly increased the antioxidative defense capacity, serum biochemical indices, and expressions of certain antioxidant-related genes, thereby helping maintain the metabolic homeostasis of the hens in late peak egg-laying period.

     

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