高效液相色谱法同时测定鱼油中维生素A、D和E

Simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography

摘要: 试验采用高效液相色谱法同时测定鱼油中维生素A、D和E,以保留时间和特征光谱图进行定性,以紫外最强吸收波长的色谱图的峰面积进行定量分析。结果表明,该方法回收率为89.6%～95.0%,相对标准偏差为3.6%～5.1%,可适用于鱼油中维生素A、D和E的测定。

Abstract: A method for the simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography was developed.The quantitative analysis on the vitamin content in fish oil was made by characterizing the retention time and spectral graph, and using the maximum absorbance wavelength for peak area.The results showed that the average recovery was 89.6%—95.0%,and variety was 3.6%—5.1%.So this method was suitable to be used in the determination on vitamin A, D and E.