摘要:
采用RT-PCR方法从番鸭肺脏器官中扩增IFN-基因片段,将扩增产物经胶回收后克隆到pMD18-T载体上,经PCR和双酶切鉴定为阳性后进行序列测定,并用Lasergene v7.1DNAStar软件对测序结果进行分析。结果表明,所扩增的IFN-基因编码完整的开放阅读框,基因长度为576bp,为国内外首次报道。并与GenBank中登录的各品种鸭、鹅IFN-基因核苷酸序列进行同源性比较分析,其同源性分别在97.0%～97.7%和95.5%～97.7%;遗传进化分析表明,鸭和鹅IFN-基因在遗传进化上呈各自独立的分支,推测IFN-基因在不同动物中有严格的种属特异性。
Abstract:
Reverse transcription-polymerase chain reaction(RT-PCR)method was used to amplified the IFN- gene fragment from lung tissue of Muscovy duck,then cloned to pMD18-T vector,the positive clones were confirmed by PCR and restriction enzyme digestion,the obtained sequence were analyzed by the Lasergene v7.1 DNAStar software.The results showed that,the Muscovy duck IFN- gene encoding a 576 bp open reading frame(ORF),which was first reported in the world.The nucleotide had higher similarity ranging from 95.5% to 97.7% among different goose species,also had higher similarity ranging from 97.0% to 97.7% among different duck species;however,the ducks and geese IFN- gene had its' own phylogentic lineage,which means,the IFN- gene had strictly specificity in different species.
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