Abstract: The main factors of protocorm-like body (PLB) induction of Onc.Sweet Sugarr were studied by using new shoots in different growth periods of the plant.The results showed that the terminal and the lateral buds of the semi-developed new shoots were better explants than others.The suitable method for the explant surface sterilization was to apply 0.1% HgCl2 for 9 minutes.After the explant became a primary plantlet,it was time to peel the shoot-tips for PLB induction.The explant survival rate could thus be significantly improved,and the PLBs could be induced in the shortest time possible.The formula of the optimal culture medium for PLB induction was the Gailang1 medium +6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1.