胡瓜钝绥螨抗阿维菌素品系的筛选及抗性稳定性分析

陈霞; 张艳璇; 季洁; 林坚贞

胡瓜钝绥螨抗阿维菌素品系的筛选及抗性稳定性分析

福建省农业科学院植物保护研究所,福建福州 350013

基金项目:

福建省自然科学基金项目(2010J01108);福建省科技计划项目省属公益类科研院所基本科研专项(2009R10028-1);福建省科技创新平台建设计划(2008N2002)

详细信息

作者简介:
陈霞(1975-),女,硕士,副研究员,研究方向为生物防治研究(E-mail:chenxiahi@sohu.com)

通讯作者:
张艳璇(1957-),女,研究员,博士,主要从事农、林害螨生物防治研究与应用(E-mail:xuan7616@sina.com)

中图分类号: S 476.2
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出版历程
- 收稿日期: 2011-05-25
- 修回日期: 2011-07-27
- 刊出日期: 2011-10-14

Selection for Avermectin Resistance and Resistance Stability in Neoseiulus cucumeris

Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350013, China

摘要

摘要: 为了使以螨治螨生防技术与病虫害综合治理中的其他防治方法合理结合,通过用玻片浸渍法对不同阿维菌素产品进行了毒力测定,确定了阿维菌素类农药对胡瓜钝绥螨具有中等毒性后,用在室内繁殖的多年未接触过任何农药的胡瓜钝绥螨敏感品系为材料,在实验室内用阿维菌素原粉进行胡瓜钝绥螨抗阿维菌素品系的汰选,并对筛选出的抗性品系进行抗性衰退、抗性再增长研究。研究结果表明,胡瓜钝绥螨敏感品系经过药剂汰选18次繁育54代后,LC50值由敏感品系的2.527 0 mgL-1上升到163.293 0 mgL-1,该胡瓜钝绥螨抗性品系的抗性倍数是敏感品系的64.62倍;抗性品系的抗性发展趋势符合逻辑斯蒂模型,拟合方程为y=120.4141/;筛选出的抗性品系对阿维菌素的抗性不稳定,繁育60代后抗性衰退至原有抗性的78.46%,60代到120代衰退缓慢,抗性水平最后稳定在敏感品系的10倍以上;当抗性衰退到原有水平的1/5左右时,再用阿维菌素原粉药剂汰选8次饲养24代后,抗性迅速上升至敏感品系的43.72倍。研究结果说明胡瓜钝绥螨对阿维菌素的抗性发展趋势发展迅速,筛选出的胡瓜钝绥螨抗阿维菌素品系即使停止用药后抗药性会较长时间保持在中等抗性水平;该抗性品系在田间应用可降低生物防治与化学防治之间的矛盾,为综合防治创造有利的条件。
- 胡瓜钝绥螨 /
- 敏感品系 /
- 抗性品系 /
- 阿维菌素 /
- 毒力测定
Abstract: The predacious mite Neoseiulus cucumeris is an important natural enemy of many pest and spider mite. However, this predator is very susceptible to insecticides such as avermectin, which is commonly used in the orchard. Selected the avemectin resistant strain by using the slide-dip method and the original population were from the population, which reared in laboratory without exposed to any insecticide for a few years. The high resistance of N. cucumeris to avermectin was gained after 54 generations. The LC50 of susceptible strain was only 2.527 0 mgL-1 , however that of resistant strain was up to 163.293 0 mgL-1 .The resistant of resistant strain was 64.62-fold as large as susceptible strain. The models of resistance growth of resistant strain was as follow, y=120.4141/. A resistance recession of resistant strain after 120 generations to avemectin was studied in this paper. The results showed the resistance of resistant strain to avemectin without exposure to avemectin decreased very quickly, and the reduction rate reached 78.46% after 60 generations, and the resistance declined slowly from 60 generations to 120 generations, and the resistant was 10-fold as large as susceptible strain. Furthermore, when the resistance declined to 20% percent of the original resistant level, the resistance re-growth was also studied in this paper. The results suggested that the resistance went up quickly after using avemectin about 24 generations; and the resistant was 43.73-fold of that of susceptible strain. The results suggested that the cultivated resistant strain which applied in the field will reduce the conflict between biological control and chemical control.
- Neoseiulus cucumeris /
- susceptible strain /
- resistant strain /
- avermectin /
- toxicity determination

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参考文献(11)

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