Abstract: Effect of hormone application on tissue culture of Trichosanthes kirilowii Maxim was investigated. Stems with axillary buds were used as explantlet,and MS for the basic medium. A vailable methods for the rapid propagation to clone T. kirilowii Maxim are reviewed.In the experiment, the young adventitious buds continuously appeared for 15 days in the culture medium of MS + 1.0 mgL-1 BA. In 30 days, the average number of shoots was 6.5, and the average shoot ratio was 81.25%. The excised plantlets could produce root 15 days after inoculation on the MS + 0.4 mg NAA L-1 medium. They continued to gradually develop into normal plants. A single plant had an average of 5 roots. The average rooting percentage was 100%. This rapid propagation method for T. kirilowii Maxim appeared to offer many benefits at low cost.