Abstract: 【Objective】Basic helix loop helix (bHLH) proteins are an important transcription factor in eukaryotes, playing a crucial role in plant growth, development, and stress response. This study cloned the bHLH transcription factor from Dendrobium officinale, predicted the structure and function of the protein, and analyzed the expression pattern of the gene under different conditions, laying the foundation for systematically elucidating the gene function.【Methods】This study cloned the DobHLH63 gene using RT-PCR technology and analyzed it using bioinformatics methods. Detecting the spatiotemporal expression pattern of DobHLH63 gene and its response to adversity through qRT-PCR technology.【Results】The coding region of DobHLH63 gene is 966bp in length and encodes 321 amino acids, belonging to the bHLH protein family. No obvious signal peptide or transmembrane domain, possibly located in the nucleus and cytoplasm, with 29 phosphorylation sites. DobHLH63 has the closest genetic relationship with PdbHLH94 (Phoenix Dactylifera), and the WD40 transcription factor may be an important interacting protein. The results of qRT-PCR showed that the expression level of DobHLH63 gene was higher in leaves than in stems. The expression of this gene can respond to various abiotic stresses and hormone treatments, especially alkaline salt stress and salicylic acid treatment.【Conclusion】The DobHLH63 gene can regulate the response of Dendrobium officinale to abiotic stress and hormones by binding to transcription factors such as WD40.