RAPD Analysis of Genetic Diversity for Capsicum annuum L.Germplasms
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摘要: 利用RAPD分子标记技术对90份辣椒种质资源遗传多样性进行分析,从200个RAPD引物中筛选出10个引物分别对供试材料的DNA进行扩增,共扩增出70个位点,其中多态性谱带38条,多态性程度为54.3%。平均每个引物产生7条多态性谱带,每个引物可扩增出4~9条,谱带大小为100~2 000 bp。对RAPD结果进行聚类分析,在D=0.33时,可以将供试的90份辣椒种质聚类为五大类群:第一类有85个种质;第二类有1个种质,是福建沙县的中牛角;第三类仅福建永安的永安黄椒1个种质;第四类有2个种质,分别是亚蔬的小尖椒PBC1752和PBC81;第五类有1个品种,是亚蔬的小线椒PBC932-6-2。品种间的亲缘关系较近,遗传基础较为狭窄。Abstract: Genetic polymorphisms of 60 Capsicum annuum L.cultivars were analyzed by using RAPD. Ten from 200 primers were selected for amplification. Seventy DNA fragments and 38 bands showed polymorphisms that occupied 54.3% of the entire amplified bands. Each primer could amplify 4 to 9,averaging 7 bands, polymorphic bands.Most of the amplified fragments had a length ranging from 100 to 2 000 bp. The cluster analysis using DPS software showed that the tested materials could be categorized into 5 groups at D=0.33. The first group included 85 varieties; the second group had only one species, i.e., MLG horn from Shaxian, Fujian; the third group had also only the yellow pepper from Yongan, Fujian; the fourth group contained two cultivars, the small greens hot pepper PBC1752 and PBC81; and the last group, Xiaoxian pepper, PBC932-6-2. All of these speices were genetically closely related with little differentiations among them.
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Keywords:
- Capsicum annuum L. /
- RAPD /
- genetic diversity /
- germplasms
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