高效液相色谱法测定欧洲鳗鲡肌肉中磺胺嘧啶和磺胺甲噁唑残留

林丽聪

高效液相色谱法测定欧洲鳗鲡肌肉中磺胺嘧啶和磺胺甲噁唑残留

林丽聪

福建省淡水水产研究所,福建福州 350002

基金项目:

福建省科技计划项目省属公益类科研院所基本科研专项(2010R1001-5)

详细信息

作者简介:
林丽聪(1982-),女,硕士,工程师,主要从事药物分析及药代动力学研究(E-mail:linlicong82@163.com)

中图分类号: S 859.84
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出版历程
- 收稿日期: 2011-07-06
- 修回日期: 2011-08-15
- 刊出日期: 2011-10-14

Simultaneous Determination of Sulfadiazine and Sulfamethoxazole Residues in Muscle of European Eels (Anguilla anguilla)by HPLC

LIN Li-cong

The Freshwater Fisheries Research Institute of Fujian Province,Fuzhou,Fujian 350002,China

摘要

摘要: 建立了欧洲鳗鲡肌肉中磺胺嘧啶和磺胺甲噁唑的高效液相色谱检测方法。均质肌肉样品采用乙腈∶氯仿(10∶1)提取,提取液经氮吹浓缩,残渣用2 mL流动相溶解,饱和正己烷脱脂净化,采用Eclipse XDB-C18 色谱柱,以乙腈∶0.05 molL-1磷酸二氢铵(20∶80)为流动相, 多波长紫外检测器检测。磺胺嘧啶和磺胺甲噁唑的检出限分别为4、8 gkg-1,在0.01~5.0 gg-1添加范围内,平均回收率均大于80%,日内与日间RSD均小于5%,说明方法满足常规残留检测要求。
- 鳗鲡 /
- 磺胺嘧啶 /
- 磺胺甲噁唑 /
- 高效液相色谱法
Abstract: A high performance liquid chromatographic method was developed for the simultaneous determination of sulfadiazine and sulfamethoxazole in European eels (Anguilla anguilla) muscle. Homogenized tissue were extracted with 10% sodium sulfate and acetonitrile- Chloroform(10∶1), the extract was concentrated by nitrogen, and redissolved into 2 mL mobile phase and defatted with n-hexane. Sulfadiazine and sulfamethoxazole were separated on Eclipse XDB-C18 Column with mobile phases containing acetonitrile and 0.05 molL-1 ammonium dihydrogen phosphate (20∶80) and finally determined by Multiple Wavelength Detector (MWD). The detection limit of sulfadiazine and sulfamethoxazole were 4 gkg-1and 8 gkg-1.In the range of 0.01~5.0 gkg-1, the mean recoveries were more than 80%. The intraday and interday precisions were less than 5%. It showed that the method meet the conventional residue testing requirements.
- Anguilla anguilla /
- sulfadiazine /
- sulfamethoxazole /
- HPLC