不同基因型禽I型副粘病毒致病性及全基因组序列分析

傅光华; 刘友生; 黄瑜; 程龙飞; 施少华; 陈红梅; 万春和; 林芳; 林建生

不同基因型禽I型副粘病毒致病性及全基因组序列分析

福建省农业科学院畜牧兽医研究所,福建福州 350012

基金项目:

国家自然科学基金项目(39072203);福建省自然科学基金项目(2009J01097);现代农业产业技术体系建设专项资金(CARS-43);福建省农业科学院双百行动项目(Sbmx1104);福建省种业创新专项(2011)

详细信息

作者简介:
傅光华(1977- ),男,博士,主要从事动物病毒分子生物学研究

通讯作者:
黄瑜(1965- ),男,博士,研究员,硕士生导师, 主要从事动物传染病研究(E-mail:huangyu_815@163.com)

中图分类号: S 852
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出版历程
- 收稿日期: 2011-09-18
- 修回日期: 2011-10-09
- 刊出日期: 2011-10-14

Pathogenicity and Genome Sequence Analysis of Different Genotype of Avian Paramyxovirus Type 1

Institution of Animal Husbandry and Veterinary Medicine of Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350012,China

摘要

摘要: 为了解当前流行于鸭群的禽I型副粘病毒的致病性和分子特征,测定分析了两株病毒对不同动物的致病性、病毒的遗传进化地位及其全基因组序列。结果表明,所分离的两株病毒中,M4毒株与疫苗Ulster株同源性最高,为基因I型,基因组全长15 186 nt,PX2/03毒株则与近来分离自水禽的FP1/02等高致病分离株的同源性最高,为基因VIII型,基因组全长15 192 nt。尽管两株病毒间的基因组核苷酸同源性很高,为92.3%,但二者致病性差异明显,毒株M4感染SPF鸡和鸭均未引起明显发病症状,而PX2/03毒株对1日龄雏鸡、6周龄SPF鸡及不同品种的雏鸭都表现出高致病性。另外,动物感染试验表明,尽管PX2/03毒株对3种鸭的致死性差异不明显,番鸭较半番鸭及樱桃谷鸭对PX2/03毒株更易感。
- 基因型 /
- 鸭源 /
- 禽I型副粘病毒 /
- 基因组序列分析
Abstract: To keep track of the pathogenicity and genomic features of avian paramyxovirus type 1 prevailing in ducks, virulence to different animals and genome sequences of two isolates as well as its genetic diversity and evolution were determined. Phylogenetic analysis showed that M4 shared a high identity with vaccine strain Ulster and belonged to genotype I, while PX2/03 isolate clustered with those virulent waterfowl isolates, such as FP1/02, which were belong to genotype VIII. Genome sequencing results showed that the genome size of M4 is 15186 bp, which is six bp shorter than that of PX2/03, and despite the fact that the two isolates shared a high identity of 92.3% with each other, they distinct significantly in pathogenicity. M4 strain was almost nonpathogenic while PX2/03 was highly pathogenic to chicken and different species of ducks. Additional, animal experiment results indicated that Muscovy duck was more susceptible to PX2/03 isolate than Mule duck or cherry valley duck does despite the similar fatality of these animals after infected with strain PX2/03.
- genotype /
- duck origin /
- avian paramyxovirus type 1 /
- genome sequence analysis

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参考文献(6)

SEAL B S, WISE M G, PEDERSEN J C, et al. Genomic sequences of low-virulence avian paramyxovirus-1 (Newcastle disease virus) isolates obtained from livebird markets in North America not related to commonly utilized commercial vaccine strains[J]. Vet Microbiol, 2005, 106(1-2):7-16.

QIN Z M, TAN L T, XU H Y, et al. Pathotypical characterization and molecular epidemiology of Newcastle disease virus isolates from different hosts in China from 1996 to 2005[J].J Clin Microbiol, 2008, 46(2):601-611.

PATTI J, MILLER A, EDUARDO LUCIO DECANINI B, et al. Newcastle disease: Evolution of genotypes and the related diagnostic challenges[J].Infection, Genetics and Evolution, 2010, 10:26-35.

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不同基因型禽I型副粘病毒致病性及全基因组序列分析

作者简介: 傅光华(1977- ),男,博士,主要从事动物病毒分子生物学研究

通讯作者: 黄瑜(1965- ),男,博士,研究员,硕士生导师, 主要从事动物传染病研究(E-mail:huangyu_815@163.com)

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出版历程

Pathogenicity and Genome Sequence Analysis of Different Genotype of Avian Paramyxovirus Type 1

计量

出版历程

目录

作者简介:
傅光华(1977- ),男,博士,主要从事动物病毒分子生物学研究

通讯作者:
黄瑜(1965- ),男,博士,研究员,硕士生导师, 主要从事动物传染病研究(E-mail:huangyu_815@163.com)