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木霉菌及其代谢产物对香蕉枯萎病菌的离体抑制作用研究

甘林, 陈福如, 杨秀娟, 阮宏椿, 杜宜新

甘林, 陈福如, 杨秀娟, 阮宏椿, 杜宜新. 木霉菌及其代谢产物对香蕉枯萎病菌的离体抑制作用研究[J]. 福建农业学报, 2010, 25(4): 462-467.
引用本文: 甘林, 陈福如, 杨秀娟, 阮宏椿, 杜宜新. 木霉菌及其代谢产物对香蕉枯萎病菌的离体抑制作用研究[J]. 福建农业学报, 2010, 25(4): 462-467.
GAN Lin, CHEN Fu-ru, YANG Xiu-juan, RUAN Hong-chun, DU Yi-xin. In vitro study on inhibition of Trichoderma sp. and their metabolites against Fusarium oxysporum f. sp. cubense[J]. Fujian Journal of Agricultural Sciences, 2010, 25(4): 462-467.
Citation: GAN Lin, CHEN Fu-ru, YANG Xiu-juan, RUAN Hong-chun, DU Yi-xin. In vitro study on inhibition of Trichoderma sp. and their metabolites against Fusarium oxysporum f. sp. cubense[J]. Fujian Journal of Agricultural Sciences, 2010, 25(4): 462-467.

木霉菌及其代谢产物对香蕉枯萎病菌的离体抑制作用研究

基金项目: 

福建省科技计划重大专项专题(2006NZ0002-3);福建省科技厅平台建设项目(2009N2005);福建省农业科学院青年基金项目(2008QB-9)

详细信息
    通讯作者:

    杨秀娟(1972- ),女,硕士,副研究员,主要从事植物真菌及病害防治研究(E-mail:yxjzb@126.con)

  • 中图分类号: S436

In vitro study on inhibition of Trichoderma sp. and their metabolites against Fusarium oxysporum f. sp. cubense

  • 摘要: 离体测定了22株木霉菌及其代谢产物对香蕉枯萎病菌4号生理小种的抑制作用,结果表明采用平板对峙培养,木霉菌对香蕉枯萎病原菌菌丝生长的抑制率为61.76%~100.00%,20%(V/V)木霉菌发酵液对病原菌菌丝生长抑制率为11.83%~52.69%,而木霉菌产生的非挥发性代谢产物和挥发性代谢产物对病原菌菌丝生长抑制率分别为3.83%~74.62%和11.17%~29.37%。用20%~50%(V/V)木霉菌T05-049发酵液处理香蕉枯萎病菌球型分生孢子9 h和镰刀型分生孢子6 h,其孢子萌发抑制率分别为86.60%~96.40%和56.29%~85.41%。
    Abstract: Inhibition effect of 22 strains of Trichoderma sp.and their metabolites against Fusarium oxysporum f.sp.cubense race four(FOC)was studied in vitro.The bioassay data obtained indicated that the inhibition rates of(a) the tested strains on the FOC mycelia growth in the plate-culture experiments ranged from 61.76% to 100%,(b) the 20%(V/V) T.sp.culture filtrates were 11.83%-52.69%,(c) the non-volatile and volatile metabolites were 3.83%-74.62% and 11.17%-29.37%,respectively,and(d) the spherical conidia spore germination from FOC treated with 20%-50%(V/V) of T.05-049 culture filtrate for 9h and the sickle-shaped conidia for 6h were 86.60%-96.40% and 56.29%-85.41%,respectively.
  • 王芊.木霉菌在生物防治上的应用及拮抗机制[J].黑龙江农业科学,2001(1):41-42.
    马红娟,杨秀娟,阮宏椿,等.拮抗细菌对香蕉枯萎病菌的离体抑菌活性研究[J].福建农业学报,2008,23(3):251-254.
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出版历程
  • 收稿日期:  2010-04-14
  • 修回日期:  2010-06-21
  • 刊出日期:  2010-08-14

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