蝴蝶兰V31品种原球茎诱导增殖培养研究

Studies on the induction and propagation for the Phalaenopsis "V31" of PLB

摘要: 以蝴蝶兰红花品系V31品种的原球茎(PLB)为材料,比较不同培养基(MS、1/2MS、改良VW、White)、不同添加物(椰子汁、香蕉汁、马铃薯汁、胰蛋白胨)和不同浓度的6-BA(0、0.5、1.0、1.5、2.0mgL-1)对蝴蝶兰PLB增殖的影响,结果表明:试验中蝴蝶兰V31品种原球茎诱导最佳培养基组合为MS+0.5mgL-16-BA+0.2mgL-1NAA+4%蔗糖+0.2%活性炭(AC)+1.0%琼脂;原球茎继代增殖培养以1/2MS+1.0mgL-16-BA+0.2mgL-1NAA+3%蔗糖+100mgL-1椰乳+0.2%活性炭(AC)+1.0%琼脂最佳.

Abstract: The effects of different basic media(MS、1/2MS、VW、White),different compounds(coconut milk,potato,banana and tryptone)and different 6-BA concentrations(0 mgL-1、0.5 mgL-1、1.0 mgL-1、1.5 mgL-1、2.0 mgL-1)were studied by adopting Phalaenopsis "V31" PLB for explant culture.The results indicated that the optimum induction combination of the Phalaenopsis "V31" PLB was MS+0.5 mgL-1 6-BA+0.2 mgL-1 NAA+4% sucrose+0.2% activated carbon+1.0% agar;the optimum propagation of the PLB was 1/2MS+1.0 mgL-1 6-BA+0.2 mgL-1 NAA+3% sucrose+0.2% activated carbon+1.0% agar.