Abstract: The gene coding for green fluorescent protein(GFP) was successfully transferred to Fusarium oxysporum f.sp.niveum strain,FOV-135,by using the protoplast-mediated genetic transformation system.The transformants were stable after five successive subcultures.Four to six stable transformants could be obtained from one microgram of plasmid DNA.The PCR amplification confirmed that the gfp gene had been transformed to FOV-135.